[1]刘金安,刘相文,耿巧玉,等.金樱子不同提取方法提取物体外抑制病毒活性及机制研究[J].西部中医药,2020,33(01):9.[doi:10.12174/j.issn.1004-6852.2020.01.03]
　LIU Jinan,LIU Xiangwen,GENG Qiaoyu,et al.Study on the Mechanism and In-vitro Virus-inhibitory Activity of the Extracts from Jinyingzi by Different Extraction Methods[J].Western Journal of Traditional Chinese Medicine,2020,33(01):9.[doi:10.12174/j.issn.1004-6852.2020.01.03]
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金樱子不同提取方法提取物体外抑制病毒活性及机制研究
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
33
期数:
2020年01期
页码:
9
栏目:
出版日期:
2020-01-15

文章信息/Info

Title:
Study on the Mechanism and In-vitro Virus-inhibitory Activity of the Extracts from Jinyingzi by Different Extraction Methods
文章编号:
1004-6852(2020)01-0009-04
作者:
刘金安1，2，刘相文1，耿巧玉1，范路路1，李思齐1，田景振1△
1 山东中医药大学药学院，山东 济南 250355； 2 青岛市中医医院
Author(s):
LIU Jinan1，2, LIU Xiangwen1, GENG Qiaoyu1, FAN Lulu1, LI Siqi1, TIAN Jingzhen1△
1 College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China;2 Qingdao City Hospital of Traditional Chinese Medicine
关键词:
金樱子; 甲醇提取液; 丙醇提取液; 抗病毒; 呼吸道合胞病毒; I型单纯性疱疹病毒; 人喉癌上皮细胞
Keywords:
Jinyingzi;  methanol extract;  propyl alcohol extract;  antiviral;  RSV;  HSV-1;  Hep-2
分类号:
R281.5
DOI:
10.12174/j.issn.1004-6852.2020.01.03
文献标志码:
A
摘要:
目的：考察金樱子不同提取物对呼吸道合胞病毒（respiratory syncytial virus，RSV）、I型单纯性疱疹病毒（herpes simplex virus type 1，HSV-1）的预防、直接杀灭和穿入人喉癌上皮细胞（human larynx carcinoma epithelial cell，Hep-2）感染的阻断作用。方法：运用体外抗病毒实验CPE法结合CCK-8染色法，以EC50、TC50及TI值作为指标，探讨金樱子甲醇、丙醇提取液对RSV、HSV-1及其穿入Hep-2感染的体外抑制活性及作用机制。结果：金樱子甲醇冷浸提取物对RSV和HSV-1的TI值分别为15.958、7.814；金樱子甲醇超声提取物对RSV和HSV-1的TI值分别为10.455、15.221；丙酮冷浸提取物对RSV的TI值为7.191；丙酮超声提取物对RSV的TI值为6.648。金樱子甲醇冷浸提取物在阻断HSV-1穿入后感染中TI值为5.650；金樱子甲醇超声提取物在阻断HSV-1穿入后感染中TI值为3.185。结论：金樱子提取物在体外能够抑制RSV、HSV-1对Hep-2细胞的感染，且金樱子的甲醇提取物作用机制复杂，有待进一步探究。
Abstract:
Objective: To investigate the preventive effects of different extracts from Jinyingzi (rosae laevigatae fructus) on RSV and HSV-1, the direction kill and the blocking effects of the extracts on Hep-2 infection after viral penetration Methods: In-vitro anti-viral experiment CPE method combined with CCK-8 staining method were adopted to explore in-vitro inhibitory activity and the mechanism of methanol extract and propyl alcohol extract of Jinyingzi on RSV, HSV-1 and Hep-2 infection after viral penetration by taking EC50, TC50 and TI values as the indexes.Results: TI values of Jinyingzi methanol cold leaching solution on RSV and HSV-1 were 15.958 and 7.814; TI values of Jinyingzi methanol ultrasonic extract on RSV and HSV-1 were 10.455 and 15.221 respectively; TI value of propyl cold alcohol extract on RSV was 7.191; TI value of propyl ultrasonic extract on RSV was 6.648. TI value of Jinyingzi methanol cold leaching solution on HSV-1 infection after blocking viral penetration was 5.650; TI value of Jinyingzi methanol ultrasonic extract on HSV-1 infection after blocking viral penetration was 3.185. Conclusion: The extracts from Jinyingzi could inhibit the infection of RSV, HSV-1 and Hep-2 cells, and the mechanism of Jinyingzi methanol extract is complicated, which needs to be further explored.

备注/Memo

备注/Memo:
收稿日期：2018-12-27*基金项目：国家科技重大专项课题(2017ZX09301030)。作者简介：刘金安(1992—)，女，硕士学位。研究方向：中药学。△通讯作者：田景振(1957—)，男，博士研究生导师，教授。研究方向：中药学。
更新日期/Last Update: 2020-01-15