[1]陈灵,周汉明△.五味子乙素介导Traf6/NF-κB信号通路抑制心肌细胞肥大实验研究[J].西部中医药,2020,33(01):33.[doi:10.12174/j.issn.1004-6852.2020.01.09]
 CHEN Ling,ZHOU Hanming.Experimental Research on Inhibitory Effects of Schisandrin B on Cardiomyocyte Hypertrophy through Mediating Traf6/NF-κB Signal Channel[J].Western Journal of Traditional Chinese Medicine,2020,33(01):33.[doi:10.12174/j.issn.1004-6852.2020.01.09]
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五味子乙素介导Traf6/NF-κB信号通路抑制心肌细胞肥大实验研究
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
33
期数:
2020年01期
页码:
33
栏目:
出版日期:
2020-01-15

文章信息/Info

Title:
Experimental Research on Inhibitory Effects of Schisandrin B on Cardiomyocyte Hypertrophy through Mediating Traf6/NF-κB Signal Channel
文章编号:
1004-6852(2020)01-0033-04
作者:
陈灵周汉明△
武汉大学附属同仁医院/武汉市第三医院药学部,湖北 武汉 430060
Author(s):
CHEN Ling, ZHOU Hanming△
Department of Pharmacy, Tongren Hospital of Wuhan University(Wuhan Third Hospital), Wuhan 430060, China
关键词:
五味子乙素心肌细胞肥大Traf6/NF-κB信号通路白细胞介素6肿瘤坏死因子α实验研究
Keywords:
schisandrin B cardiomyocyte hypertrophy Traf6/NF-κB signal channel IL-6 TNF-αexperimental study
分类号:
R542
DOI:
10.12174/j.issn.1004-6852.2020.01.09
文献标志码:
A
摘要:
目的:研究五味子乙素抑制脂多糖(lipopolysaccharide,LPS)诱导心肌细胞肥大的作用及其机制。方法:原代培养心肌细胞,用LPS(1 mg/L)诱导心肌细胞肥大,考察IκBα抑制剂BAY11-7082和不同剂量五味子乙素对心肌细胞肥大的影响。采用计算机图像分析系统观察细胞大小;考马斯亮蓝法分析细胞总蛋白量;ELISA试剂盒检测白细胞介素6(interleukin-6,IL-6)和肿瘤坏死因子α(tumor necrosis factor α,TNF-α)表达量;免疫印迹技术检测心房钠尿肽(atrial natriuretic peptide,ANP)和Traf6/NF-κB信号通路蛋白表达水平。结果:与LPS诱导组相比,五味子乙素和BAY11-7082均可以抑制LPS诱导的心肌细胞肥大,体现在蛋白含量降低,细胞体积减小,ANP蛋白表达降低(P<0.05);五味子乙素还能够降低炎症反应,表现在细胞外液中炎症因子IL-6和TNF-α水平降低(P<0.05),Traf6、p65蛋白表达量降低(P<0.05)及IκBα蛋白水平升高(P<0.05),且呈浓度依赖性。此外,高剂量五味子乙素与BAY11-7082对心肌细胞肥大的保护作用相近。结论:五味子乙素能抑制LPS诱导原代心肌细胞肥大,对心肌细胞的保护作用主要通过抑制Traf6/NF-κB信号通路的活化来实现。
Abstract:
Objective: To investigate the effects and mechanism of schisandrin B on cardiomyocyte hypertrophy induced by lipopolysaccharide (LPS). Methods: Cardiomyocyte hypertrophy was induced by LPS (1 mg/L) after culturing primary cardiomyocyte, to investigate the influence of IκBα inhibitor BAY11-7082 and different dosages of schisandrin B on cardiomyocyte hypertrophy. Computer image analysis system was used to observe the size of the cells; coomassie blue staining to analyze total protein contents of the cells; ELISA kit to detect the expressions of IL-6 and TNF-α; immunoblot assay to measure the expressions of atrial natriuretic peptide (ANP) and Traf6/NF-κB signal channel protein. Results: Compared with LPS-induced group, schisandrin B and BAY11-7082 could inhibit LPS-induced cardiomyocyte hypertrophy, it reflected in protein contents reduction and cellular volume decrease, and ANP protein expressions reduction (P<0.05); schisandrin B could relieve inflammatory reaction, and it perfomed in reducing the levels of IL-6 and TNF-α in extracellular fluid (P<0.05); lowering the expressions of Traf6 and p65 protein (P<0.05) and lifting the levels of IκBα protein (P<0.05), in the concentration dependent maner. Besides,high dose of schisandrin B showed similar protective effects in cardiomyocyte hypertrophy to BAY11-7082. Conclusion: Schisandrin B could inhibit LPS-induced primary cardiomyocyte hypertrophy, its protection for cardiomyocyte is realized mainly through inhibiting Traf6/NF-κB signal channel activation.

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备注/Memo

备注/Memo:
收稿日期:2018-10-12*基金项目:武汉市卫生健康科研基金(WZ19Z014)。作者简介:陈灵(1984—),女,硕士学位,主管药师。研究方向:中药药理学。△通讯作者:周汉明(1976—),男,硕士学位,副主任药师。研究方向:中药药理学。
更新日期/Last Update: 2020-01-15