[1]周罡,郭耀宇,袁雪婷,等.国家自然科学基金资助国内中医药领域动物实验研究现状分析[J].西部中医药,2020,33(01):79.[doi:10.12174/j.issn.1004-6852.2020.01.21]
　ZHOU Gang,GUO Yaoyu,YUAN Xueting,et al.Status Analysis of Animal Experiments in TCM Field Funded by National Natural Science Foundation of China[J].Western Journal of Traditional Chinese Medicine,2020,33(01):79.[doi:10.12174/j.issn.1004-6852.2020.01.21]
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国家自然科学基金资助国内中医药领域动物实验研究现状分析
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
33
期数:
2020年01期
页码:
79
栏目:
出版日期:
2020-01-15

文章信息/Info

Title:
Status Analysis of Animal Experiments in TCM Field Funded by National Natural Science Foundation of China
文章编号:
1004-6852(2020)01-0079-06
作者:
周罡1，郭耀宇2，3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' target="_blank" rel="external">3' 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1 甘肃省人民医院干部病房心内科，甘肃 兰州 730000； 2 兰州大学循证医学中心/兰州大学基础医学院； 3 兰州大学第一临床医学院； 4 兰州大学护理学院； 5 兰州大学公共卫生学院
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rel="external">3' target="_blank" rel="external">?3' target="_blank" rel="external">3' target="_blank" rel="external">?3' target="_blank" rel="external">3' target="_blank" rel="external">?3, XU Jiake2,3' target="_blank" rel="external">?3' target="_blank" rel="external">3' target="_blank" rel="external">?3' target="_blank" rel="external">3' target="_blank" rel="external">?3' target="_blank" rel="external">3' target="_blank" rel="external">?3' target="_blank" rel="external">3' target="_blank" rel="external">?3' target="_blank" rel="external">3' target="_blank" rel="external">?3' target="_blank" rel="external">3' target="_blank" rel="external">?3' target="_blank" rel="external">3' target="_blank" rel="external">?3' target="_blank" rel="external">3' target="_blank" rel="external">?3' target="_blank" rel="external">3' target="_blank" rel="external">?3' target="_blank" rel="external">3' target="_blank" rel="external">?3' target="_blank" rel="external">3' target="_blank" rel="external">?3' 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target="_blank" rel="external">?3' target="_blank" rel="external">3' target="_blank" rel="external">?3' target="_blank" rel="external">3' target="_blank" rel="external">?3' target="_blank" rel="external">3' target="_blank" rel="external">?3' target="_blank" rel="external">3' target="_blank" rel="external">?3' target="_blank" rel="external">3' target="_blank" rel="external">?3, MA Bin2△
1 Department of Cardiology of the Cadre Ward, Gansu Provincial Hospital, Lanzhou 730000, China;2 Evidence Based Medicine Center/School of Basic Medical Sciences, Lanzhou University; 3 The First Clinical Medical College, Lanzhou University; 4 The Nurs
关键词:
国家自然科学基金; 中医药; 动物实验; 方法质量; 报告质量
Keywords:
national natural science foundation of China;  TCM;  animal experiments;  methodological quality;  report quality
分类号:
R285.5
DOI:
10.12174/j.issn.1004-6852.2020.01.21
文献标志码:
A
摘要:
目的：全面收集由国家自然科学基金(national natural science foundation of China，NSFC)资助的发表在国内期刊的中医药领域内的动物实验，采用文献计量学方法，对其研究现状及存在的问题进行回顾分析，以期为今后相关研究的开展和实施提供参考依据。方法：计算机检索中国科学引文数据库(CSCD)，纳入所有NSFC资助的中医药研究领域内原始干预类动物实验，检索时间为建库至2017年3月。由2位研究者按纳入与排除标准筛选文献、提取资料，对纳入研究的特征进行统计学分析。结果：最终纳入1072个研究，1)方法质量：99.5%(1067/1072)的研究设立了对照组，93.0%(997/1072)的研究为随机对照实验，仅7.1%(71/997)的研究报告了具体随机方法，无任何研究报告实施隐蔽分组，仅0.4%(4/1072)的研究实施了盲法。2)报告质量：多数研究报告了详细的实验步骤和过程(99.8%，1070/1072)、动物选择的纳入标准(98.8%，1059/1072)和动物的数量(94.4%，1012/1072)，但少有研究明确提出主、次要测量指标(0.2%，2/1072)、报告样本量计算依据(0.3%，3/1072)以及报告研究的伦理审查情况(0.5%，5/1072)。结论：由NSFC资助的发表在国内期刊的中医药领域内的动物实验其研究人员在设计、实施阶段对一些影响其方法质量的重要因素重视度不够，报告存在不充分、质量低下等问题，最终导致其质量低下。因此，今后研究有必要：1)采取针对性措施推广与普及SYRCLE动物实验风险评估工具，有效指导动物实验的开展，提高动物实验的设计和实施。2)按照动物实验研究报告指南(ARRIVE指南)充分报告，以促进实验全过程的透明化，最终促进提高报告质量。
Abstract:
Objective: To provide the reference for the development and implementation of future related study by comprehensively collecting animal experiments in TCM field subsidized by national natural science foundation of China(NSFC) pulblished on domestic journals, retrospectively analyzing the current state and the existing problems using literaure metrology. Methods: Original intervention type animal experiments in TCM field subsidized by NSFC were searched from CSCD using computer, and included from the date of building the database to March, 2017. The papers were screened and the data was abstracted by two investigators according to the inclusion and exclusion criteria, and the characteristics of the included studies were statistically analyzed. Results: Consequently, 1072 researches were included, 1) methodological quality: 99.5% (1067/1072) of the researches have established the control group, 93.0%(997/1072) of the experiments were RCTs, only 7.1% (71/997) have reported specific randomized methods, none has reported allocation concealments and only 0.4% (4/1072) implemented blind method. 2) report quality: most of the studies have reported detailed steps and procedure (99.8%, 1070/1072), the inclusion criteria of selecting animals (98.8%, 1059/1072) and the number of the animals (94.4%, 1012/1072), but few studies raised main and secondary outcomes obviously (0.2%, 2/1072), sample size calculation basis(0.3%, 3/1072) and ethical investigation(0.5%, 5/1072). Conclusion: The researchers pay little attention to the important influencing factors on methodological quality at the phases of design and implementation, the report existed the problems of being inadequate, low quality and others in animal experiments in TCM field published on domestic journals subsidized by NSFC, finally these would cause low quality report. Hence, in furture study, it is necessary: 1) to take the targeted measures to spread and popularize SYRCLE's risk of bias tool for animal experiments, effectively guide the development of animal experiments, improve the design and the implementation of animal experiments. 2) to report adequately in light of ARRIVE guidelines, promote the hyalinization of the whole process of animal experiments, consequently, to promote the improvement of report quality.

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备注/Memo

备注/Memo:
收稿日期：2018-12-26*基金项目：国家自然科学基金项目(81303147)。作者简介：周罡(1980—)，男，硕士学位，主治医师。研究方向：心血管疾病机制及诊疗。△通讯作者：马彬(1979—)，女，硕士研究生导师，博士学位，副教授。研究方向：系统评价方法学、医学研究报告规范。
更新日期/Last Update: 2020-01-15