[1]王文秀,张普,刘倩,等.改良线栓法与传统线栓法制备MCAO大鼠模型20天存活率的比较[J].西部中医药,2020,33(04):42-44.[doi:10.12174/j.issn.1004-6852.2020.04.13]
　WANG Wenxiu,ZHANG Pu,LIU Qian,et al.An Evaluation of 20-day Survival Rates of MCAO Rat Model Prepared by Modified and Traditional Suture-occluded Methods[J].Western Journal of Traditional Chinese Medicine,2020,33(04):42-44.[doi:10.12174/j.issn.1004-6852.2020.04.13]
点击复制

改良线栓法与传统线栓法制备MCAO大鼠模型20天存活率的比较
分享到：

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
33
期数:
2020年04期
页码:
42-44
栏目:
出版日期:
2020-04-15

文章信息/Info

Title:
An Evaluation of 20-day Survival Rates of MCAO Rat Model Prepared by Modified and Traditional Suture-occluded Methods
文章编号:
1004-6852(2020)04-0042-03
作者:
王文秀，张普，刘倩，丁凡帆，康晓文，巩婷，蒲永乐，邱连利
1 甘肃中医药大学，甘肃 兰州 730000； 2 甘肃省中医院
Author(s):
WANG Wenxiu 1, ZHANG Pu 2, LIU Qian 1, DING Fanfan 1, KANG Xiaowen 1,GONG Ting 2, PU Yongle 2, QIU Lianli 2△
1 Gansu University of Chinese Medicine, Lanzhou 730000, China; 2 Gansu Provincial Hospital of Traditional Chinese Medicine
关键词:
缺血再灌注模型; 改良线栓法; 传统线栓法; 制模成功率; 存活率
Keywords:
ischemia reperfusion model;  modified sture-occluded method;  traditional sture-occluded method; modeling success rate;  survival rate
分类号:
R318.5
DOI:
10.12174/j.issn.1004-6852.2020.04.13
文献标志码:
A
摘要:
目的：通过对比传统线栓法与改良线栓法法的存活率，为MCAO大鼠模型制备提供一种更稳定的造模方法。方法:选取50只雄性SD大鼠，分为实验组25只，对照组25只，实验组予改良线栓法，对照组予传统线栓法，造模成功后分别予生理盐水灌胃，对比造模成功率、神经功能缺损评分、20天存活率。结果：模型制备成功率实验组为96.0%（24/25），对照组为92.0%（23/25），2组比较差异无统计学意义(P＞0.05)。2组神经缺损功能评分各分比率接近，差异无统计学意义(P＞0.05)。20天存活率实验组为66.67%(16/24)，对照组为31.9%(9/23)，2组比较差异有统计学意义(P＜0.05)。结论：改良线栓法制备MCAO大鼠模型存活率高于传统线栓法制备MCAO大鼠模型存活率。
Abstract:
Objective: To establish the stabilized preparation MCAO rat model via comparing the survival rates of the rats prepared by traditional and modified middle cerebral artery occlusion (MCAO). Methods: Fifty male SD rats were randomized into the experiment group and the control group, 25 rats each group, the experiment group was given modified sture-occluded method, and the control group traditional sture-occluded method, they were drenched with physiological saline respectively after successfully modeling, to compare the success rate of modeling, neurological severity score (NSS) and 20-day survival rate. Results: Model preparation success rate of the experiment group was 96.0% (24/25), higher than 92.0% (23/25) of the control group, and the difference had no statistical meaning (P＞0.05). Different score ratios of NSS in both groups approached, and the difference showed no statistical meaning (P＞0.05). 20-day survival rate of the experiment group was 66.67%(16/24), higher than 31.9%(9/23) of the control group, and the difference had statistical meaning (P＜0.05). Conclusion: The survival rate of modified MCAO rat is higher than that of traditional sture-occluded method.

备注/Memo

备注/Memo:
收稿日期：2019-04-01*基金项目：甘肃省自然科学基金计划项目(1508RJZA042)；国家中医药管理局国家中医临床研究基地业务建设科研专项课题（JDZX2015019）。作者简介：王文秀(1995—)，女，在读硕士研究生。研究方向：临床疾病的针灸。△通讯作者：邱连利(1963—)男，硕士研究生导师，主任医师。研究方向：针炙临床及教学。
更新日期/Last Update: 2020-04-15