[1]顾健沛.HPLC法同时测定锁阳中没食子酸和儿茶素的含量[J].西部中医药,2020,33(05):27-29.[doi:10.12174/j.issn.1004-6852.2020.05.07]
 GU Jianpei.Simultaneous Determination of Gallic Acid and Catechin in Suoyang by HPLC Method[J].Western Journal of Traditional Chinese Medicine,2020,33(05):27-29.[doi:10.12174/j.issn.1004-6852.2020.05.07]
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HPLC法同时测定锁阳中没食子酸和儿茶素的含量
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
33
期数:
2020年05期
页码:
27-29
栏目:
出版日期:
2020-05-15

文章信息/Info

Title:
Simultaneous Determination of Gallic Acid and Catechin in Suoyang by HPLC Method
文章编号:
1004-6852(2020)05-0027-03
作者:
顾健沛
兰州现代职业学院,甘肃 兰州 730030
Author(s):
GU Jianpei
Lanzhou Modern Vocational College, Lanzhou 730030, China
关键词:
锁阳没食子酸儿茶素HPLC
Keywords:
Suoyang gallic acid catechin HPLC
分类号:
R283
DOI:
10.12174/j.issn.1004-6852.2020.05.07
文献标志码:
A
摘要:
目的:建立锁阳中没食子酸和儿茶素含量HPLC测定方法。方法:色谱柱为Waters C18 (4.6 mm×250 mm,5 μm),以甲醇和0.1%磷酸为流动相,流速:1 mL/min,进行梯度洗脱。结果:锁阳饮片中没食子酸含量为0.01%~0.18%,儿茶素含量为0.10%~0.81%。结论:本研究建立的同时测定锁阳中没食子酸和儿茶素成分含量方法实用、简便,可为制订锁阳质量控制标准提供依据。
Abstract:
Objective: To establish a method of detecting the contents of gallic acid and catechin in Suoyang (cynomorii herba) by HPLC. Methods: Chromatographic column was waters C18(4.6mm×250 mm, 5μm), methanol-0.1% phosphoric acid as mobile phase, flow velocity:1 mL/min, to perform gradient elution. Results: The contents of gallic acid contained in Suoyang decoction pieces were 0.01%-0.18%, and the contents of catechin 0.10%-0.81%. Conclusion: The method of detecting the contents of gallic acid and catechin in Suoyang established in the study, practical and simple, could provide the reference for quality control of Suoyang.

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备注/Memo

备注/Memo:
收稿日期:2019-06-27作者简介:顾健沛(1970—),女,副教授。研究方向:药品质量分析研究。
更新日期/Last Update: 2020-05-15