[1]仇晓霞,史晓敏.基于AKT信号通路探讨紫草素对宫颈癌细胞凋亡及Cyclin-D1蛋白表达的影响[J].西部中医药,2021,34(04):1-4.[doi:10.12174/j.issn.2096-9600.2021.04.03]
　QIU Xiaoxia,SHI Xiaomin.Influence of Shikonin on the Expressions of Cyclin-D1 Protein and the Apoptosis of Cervical Cancer Cells Based on AKT Signaling Pathway[J].Western Journal of Traditional Chinese Medicine,2021,34(04):1-4.[doi:10.12174/j.issn.2096-9600.2021.04.03]
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基于AKT信号通路探讨紫草素对宫颈癌细胞凋亡及Cyclin-D1蛋白表达的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
34
期数:
2021年04期
页码:
1-4
栏目:
出版日期:
2021-04-15

文章信息/Info

Title:
Influence of Shikonin on the Expressions of Cyclin-D1 Protein and the Apoptosis of Cervical Cancer Cells Based on AKT Signaling Pathway
作者:
仇晓霞, 史晓敏
西北民族大学直属附属医院，甘肃 兰州 730000
Author(s):
QIU Xiaoxia, SHI Xiaomin
Affiliated Hospital of Northwest University for Nationalities, Lanzhou 730000, China
关键词:
紫草素; AKT信号通路; 宫颈癌细胞; Cyclin-D1蛋白
Keywords:
shikonin; AKT signaling pathway; cervical cancer cells; Cyclin-D1 protein
分类号:
R711.74
DOI:
10.12174/j.issn.2096-9600.2021.04.03
摘要:
目的研究紫草素激活AKT信号通路对宫颈癌细胞凋亡及Cyclin-D1蛋白表达的影响。 方法将宫颈癌海拉（Hela）细胞接种于DEME培养基中，根据分组，加入不同浓度的紫草素细胞培养液进行培养。分别测定培养24、48、72 h后空白组、低浓度组、中浓度组及高浓度组细胞凋亡率。另采用实时荧光定量PCR技术及Western Blotting法分别检测培养24 h后各组AKT、p-AKT及细胞周期蛋白D1信使核糖核酸（cyclin-Dlmessenger RNA，Cyclin-D1mRNA）蛋白表达量。 结果低浓度组、中浓度组、高浓度组细胞凋亡率均随着时间推移而升高，其中高浓度组最显著。细胞AKT、p-AKT、Cyclin-D1 mRNA表达量及蛋白量随着浓度的增高而减少，高浓度组明显低于其他各组（P＜0.05），中浓度组明显低于低浓度及空白组（P＜0.05），低浓度组低于空白组（P＜0.05）。 结论紫草素对宫颈癌细胞具有明显抑制作用，作用机制与其抑制AKT信号通路及Cyclin-D1表达量有关。
Abstract:
ObjectiveTo study the effects of shikonin activating AKT signaling pathway on the expressions of Cyclin-D1 protein and the apoptosis of cervical cancer cells. MethodsAfter inoculating in DEME medium, Hela cells of cervical cells were cultured by adding different concentrations of shikonin cell culture solution according to the groups. To detect cellular apoptosis rates of the blank group, low, moderate and high concentra-tions groups after culturing for 24, 48 and 72 hours respectively. Real-time PCR and Western blotting were adopted to measure the expressions of AKT, p-AKT and Cyclin-D1mRNA proteins in different groups after culturing for 24 hours respectively. ResultsThe apoptosis rate of low, moderate and high concentrations groups increased as time went by, among them, high concentrations group was the most significant. The expressions of AKT, p-AKT, Cyclin-D1 mRNA and the protein reduced as the concentrations increased, high concentration group was notably lower than other groups (P<0.05), moderate concentration group was lower than low concentration group and the blank group (P<0.05), low concentration group was lower than the blank group (P<0.05). ConclusionShikonin owns notable inhibitory effects on cervical cancer cells, and its mechanism might be related to that it could inhibit AKT signaling pathway and the expressions of Cyclin-D1 protein.

相似文献/References:

[1]赵阳.紫草素对荷肝癌大鼠免疫功能的影响[J].西部中医药,2018,31(09):26.
　ZHAO Yang.Effects of Shikonin on Immunologic Function of the Rats Suffering Liver Cancer[J].Western Journal of Traditional Chinese Medicine,2018,31(04):26.
[2]刘丛丽,董金菊,周双玉.紫草素对宫颈癌细胞株Caski侵袭迁移及Wnt/β-catenin信号通路的影响[J].西部中医药,2021,34(09):27.[doi:10.12174/j.issn.2096-9600.2021.09.08]
　LIU Congli,DONG Jinju,ZHOU Shuangyu.Effects of Shikonin on the Invasion and Migration of Cervical Cancer Cell Line Caski and Wnt/β-catenin Signaling Pathway[J].Western Journal of Traditional Chinese Medicine,2021,34(04):27.[doi:10.12174/j.issn.2096-9600.2021.09.08]

备注/Memo

备注/Memo:
仇晓霞（1972—），女，副主任医师。研究方向：妇科肿瘤的微创治疗。
更新日期/Last Update: 2021-04-15