[1]马琴国,刘效栓,罗燕燕,等.甘肃铨水掌叶大黄的HPLC谱效研究[J].西部中医药,2021,34(08):45-50.[doi:10.12174/j.issn.2096-9600.2021.08.10]
　MA Qinguo,LIU Xiaoshuan,LUO Yanyan,et al.HPLC Spectrum Effects of Zhangye Dahuang in Quanshui of Gansu[J].Western Journal of Traditional Chinese Medicine,2021,34(08):45-50.[doi:10.12174/j.issn.2096-9600.2021.08.10]
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甘肃铨水掌叶大黄的HPLC谱效研究
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
34
期数:
2021年08期
页码:
45-50
栏目:
质量分析
出版日期:
2021-08-15

文章信息/Info

Title:
HPLC Spectrum Effects of Zhangye Dahuang in Quanshui of Gansu
作者:
马琴国1,2, 刘效栓1, 罗燕燕1, 张中华1, 王引权2,3,4, 刘东玲3
1.甘肃省中医院，甘肃 兰州 730050
2.甘肃省中药质量与标准研究重点实验室培育基地
3.甘肃中医药大学
4.西北中藏药协同创新中心
Author(s):
MA Qinguo1,2, LIU Xiaoshuan1, LUO Yanyan1, ZHANG Zhonghua1, WANG Yinquan2,3,4, LIU Dongling3
1.Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China
2.Key Laboratory of Quality and Standard Research for Traditional Chinese Medicines of Gansu Province
3.Gansu University of Chinese Medicine
4.Northwest Herbal
关键词:
甘肃礼县铨水掌叶大黄; 乙醇提取物石油醚萃取物; HPLC图谱; 泻下成分谱效图; 相似度分析
Keywords:
of County; ethyl petroleum ether extract by ethanol extraction; HPLC spectrum; the spectrum of the purgative components; similarity analysis
分类号:
R285
DOI:
10.12174/j.issn.2096-9600.2021.08.10
摘要:
目的对甘肃礼县铨水掌叶大黄药材的乙醇提取乙石油醚萃取物中泻下成分进行研究，建立泻下成分高效液相色谱法（high performance liquid chromatography，HPLC）谱效图。 方法收集甘肃礼县10个乡的铨水掌叶大黄，以泻下成分大黄酸8-o-β-D葡萄糖苷、番泻苷-A，大黄酚8-o-葡萄糖苷、番泻苷-B、大黄素8-o-β-D吡喃葡萄糖为参照物，构建甘肃礼县铨水掌叶大黄泻下成分的HPLC谱效图，并进行相似度分析，建立掌叶大黄的对照谱效图。 结果建立了甘肃礼县掌叶大黄泻下成分HPLC指纹谱效图，指认了甘肃礼县铨水掌叶大黄醇提物中五种泻下成分的峰位。 结论建立的谱效图稳定性、重复性良好， 可作为甘肃礼县掌叶大黄活泻下特征参考图谱。
Abstract:
ObjectiveTo study the purgative components in ethyl petroleum ether extract by ethanol extraction from Zhangye Dahuang (Rheum palmatum L.) in Quanshui of Gansu province, and establish the HPLC spectrum of the purgative components. MethodsAfter collecting Zhangye Dahuang from ten townships of Lixian country, Gansu province, the purgative components Rhein 8-o-β-D glucoside, sennoside-a, chrysophanol 8-o-glucoside, sennoside-b, rhubarb 8-o- β- D glucopyranose were chosen as the objects of reference, HPLC spectrums of the purgative components of Zhangye Dahuang in Quanshui of Gansu province were constructed to perform the similarity analysis and establish the control spectrum of Dahuang. ResultsThe HPLC fingerprint of purgative components of Dahuang in Lixian County, Gansu Province were established to identify the peak positions of five purgative components in the alcohol extract of Dahuang in Quanshui County, Gansu Province. ConclusionThe established spectrum is stable and reproducible, and it could be used as a reference map for the purgative characteristics of Zhangye Dahuang in Lixian County, Gansu Province.

备注/Memo

备注/Memo:
马琴国（1976—），女，硕士学位，中药师。研究方向：中药质量控制。甘肃省中药质量与标准研究重点实验室培育基地资助项目（ZYZL16-008）。
更新日期/Last Update: 2021-08-15