[1]张英俊,常柏,谭力子.抵当汤早期干预对2型糖尿病大鼠血管内皮细胞黏附连接的影响[J].西部中医药,2021,34(09):18-22.[doi:10.12174/j.issn.2096-9600.2021.09.06]
　ZHANG Yingjun,CHANG Bo,TAN Lizi.Influence of Early Intervention of Didang Decoction on the Adhesion Junction of Vascular Endothelial Cells in T2DM Rats[J].Western Journal of Traditional Chinese Medicine,2021,34(09):18-22.[doi:10.12174/j.issn.2096-9600.2021.09.06]
点击复制

抵当汤早期干预对2型糖尿病大鼠血管内皮细胞黏附连接的影响
分享到：

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
34
期数:
2021年09期
页码:
18-22
栏目:
出版日期:
2021-09-15

文章信息/Info

Title:
Influence of Early Intervention of Didang Decoction on the Adhesion Junction of Vascular Endothelial Cells in T2DM Rats
作者:
张英俊1, 常柏2, 谭力子1
1.天津中医药大学，天津 301617
2.天津医科大学朱宪彝纪念医院
Author(s):
ZHANG Yingjun1, CHANG Bo2, TAN Lizi1
1.Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
2.Tianjin Medical University Chu Hisen-I Memorial Hospital
关键词:
2型糖尿病; 大血管病变; 黏附连接; 抵当汤; 大鼠
Keywords:
T2DM; macrovascular disease; adhesion junction; decoction; rats
分类号:
R587
DOI:
10.12174/j.issn.2096-9600.2021.09.06
摘要:
目的探讨抵当汤早期干预对2型糖尿病大鼠血管内皮细胞（vascular endothelial cells，VEC）黏附连接作用的影响。 方法采用投喂高脂饲料并尾静脉注射链脲佐菌素（streptozotocin，STZ）的方法建立2型糖尿病大鼠模型，将大鼠随机分为7组（正常对照组、糖尿病模型组、二甲双胍组、辛伐他汀组及抵当汤早、中、晚期干预组），采用Western-Blot法检测各组大鼠VEC钙黏蛋白（VE-Cad）表达；实时荧光定量PCR方法检测Ras相关蛋白1（RAS-related protein 1，Rap1）、膜相关鸟苷酸激酶1（MAGI1）、酪氨酸蛋白激酶2（Tie2）的表达水平；HE染色后观察各组大鼠胸主动脉结构。 结果与正常对照组比较，糖尿病模型组VE-Cad、Rap1、MAGI1、Tie2表达下降（P＜0.05）；与糖尿病模型组比较，抵当汤早、中期干预组VE-Cad、Rap1、MAGI1、Tie2表达上升（P＜0.05）；与抵当汤晚期干预组比较，早期干预组VE-Cad、Rap1、MAGI1、Tie2表达上升（P＜0.05）。 结论抵当汤早期干预可增强VEC间黏附连接，增强血管稳定性，改善血管通透性，延缓糖尿病大血管病变的发展。
Abstract:
ObjectiveTo explore the effects of early intervention of Didang decoction on the adhesion junction of VEC in T2DM rats. MethodsRat models with T2DM were established by feeding high fat feed and injecting STZ into caudal vein, randomized into seven groups (normal control group, DM model group, metformin group, simvastatin group, early, middle and late intervention groups of Didang decoction, Western blot method was adopted to detect the expressions of VE-Cad, real time fluorescence quantitative PCR to measure the expressions of Rap1, MAGI1 and Tie2; to observe thoracic aortic structure after HE staining. ResultsCompared with normal control group, the expressions of VE-Cad, Rap1, MAGI1 and Tie2 reduced in DM model group (P<0.05); compared with DM model group, the expressions of VE-Cad, Rap1, MAGI1 and Tie2 increased in early and middle intervention groups of Didang decoction (P<0.05); compared with late intervention group of Didang decoction, the expressions of VE-Cad, Rap1, MAGI1 and Tie2 rosed in early intervention group of Didang decoction (P<0.05). ConclusionEarly intervention of Didang decoction could enhance the adhesion junction of VEC, strengthen vascular stability, improve vascular permeability and delay the development of diabetic macroangiopathy.

相似文献/References:

[1]祁瑞芳.同型半胱氨酸、超敏 C- 反应蛋白在 2 型糖尿病合并脑梗死预测中的作用[J].西部中医药,2014,27(08):152.
[2]邱勇玉,李应东,赵信科,等.高血压病中医证型与 2 型糖尿病及其相关危险因素分析[J].西部中医药,2014,27(09):75.
[3]李东峰,指导：王志刚.王志刚主任医师治疗2型糖尿病“三级”防治思想辨治心得[J].西部中医药,2015,28(01):29.
[4]周雨聪,指导：刘召.红灵五黄汤治疗2型糖尿病90例[J].西部中医药,2015,28(04):99.
[5]杨国军.黄芪注射液对2型糖尿病患者血管功能及微循环状态的影响[J].西部中医药,2015,28(05):109.
[6]康学东,张瀚文.病证结合探讨2型糖尿病胰岛素抵抗[J].西部中医药,2015,28(11):112.
[7]张喜芬,赵保礼,杨立波,等.通络明目胶囊治疗糖尿病视网膜病变的随机双盲对照研究[J].西部中医药,2013,26(11):88.
　ZHANG Xifen,ZHAO Baoli,YANG Libo,et al.A Randomized, Double-blind and Placebo-controlled Study of TongLuo MingMu Capsule in Treating Diabetic Retinopathy[J].Western Journal of Traditional Chinese Medicine,2013,26(09):88.
[8]戴梅.全面干预护理对2型糖尿病患者血糖和糖化血红蛋白水平的影响[J].西部中医药,2013,26(07):109.
　DAI Mei.Intervention of Comprehensive Nursing on Blood Glucose and Glycosylated Hemoglobin of Patients with Diabetes Mellitus Type Two[J].Western Journal of Traditional Chinese Medicine,2013,26(09):109.
[9]张家林,裴瑞霞.银花痛风颗粒治疗2型糖尿病合并高尿酸血症疗效观察[J].西部中医药,2014,27(01):106.
　ZHANG Jialin,PEI Ruixia.Clinical Observation on YinHua TongFeng Granules in Treating Type 2 Diabetes Mellitus Complicated with Hyperuricemia[J].Western Journal of Traditional Chinese Medicine,2014,27(09):106.
[10]祁瑞芳.同型半胱氨酸、超敏C-反应蛋白在2型糖尿病合并脑梗死预测中的作用[J].西部中医药,2014,27(04):136.
　QI Ruifang.Diagnostic Value of Homocysteine and High Sensitivity C-Reactive Protein in Predicting Type 2 Diabetes Mellitus Complicated with Cerebral Infarction[J].Western Journal of Traditional Chinese Medicine,2014,27(09):136.

备注/Memo

备注/Memo:
张英俊（1993—），女，硕士研究生。研究方向：中医内科学。国家自然科学基金（81473622）。
更新日期/Last Update: 2021-09-15