[1]刘高宏,魏立莉,刘惠玲.近红外光谱法快速测定白芍中3种成分的含量?[J].西部中医药,2021,34(11):75-79.[doi:10.12174/j.issn.2096-9600.2021.11.15]
　LIU Gaohong,WEI Lili,LIU Huiling.Rapid Determination of Three Components in Baishao by Near-infrared Spectroscopy[J].Western Journal of Traditional Chinese Medicine,2021,34(11):75-79.[doi:10.12174/j.issn.2096-9600.2021.11.15]
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近红外光谱法快速测定白芍中3种成分的含量?
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
34
期数:
2021年11期
页码:
75-79
栏目:
质量分析
出版日期:
2021-11-15

文章信息/Info

Title:
Rapid Determination of Three Components in Baishao by Near-infrared Spectroscopy
作者:
刘高宏, 魏立莉, 刘惠玲
甘肃省中医院，甘肃 兰州 730050
Author(s):
LIU Gaohong, WEI Lili, LIU Huiling
Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China
关键词:
白芍; 近红外光谱法; 没食子酸; 芍药苷; 芍药内酯苷
Keywords:
near-infrared spectroscopy; gallic acid; paeoniflorin; albiflorin
分类号:
R284.1
DOI:
10.12174/j.issn.2096-9600.2021.11.15
文献标志码:
A
摘要:
目的建立白芍生品中没食子酸、芍药苷及芍药内酯苷含量的快速测定近红外光谱定量模型。 方法以HPLC检测值为参照，采用傅里叶变换近红外漫反射光谱技术采集白芍生品的近红外光谱，结合偏最小二乘法（PLS）分别建立没食子酸、芍药苷及芍药内酯苷含量的快速测定方法。 结果白芍中没食子酸、芍药苷及芍药内酯苷的校正模型相关系数（R）、校正均方差（RMSEC）、内部验证均方差（RMSEP）分别为0.99132、0.0055、0.00896；0.99869、0.0266、0.10900；0.97052、0.0349、0.04210。通过外部验证，白芍的预测值与真实值相关系数分别为0.9404、0.9412和0.9446。 结论该方法准确、快速、简便，可实现大批量样品的快速分析。
Abstract:
ObjectiveTo establish a quantitative model for the rapid determination of gallic acid, paeoniflorin and albiflorin in raw Baishao (Radix Paeoniae Alba) by near-infrared spectroscopy. MethodsBy taking the detection value of HPLC as the reference, Fourier transform near infrared diffuse reflectance spectroscopy was adopted to collect near-infrared spectroscopy of raw Baishao, the method for rapid determination of the contents of gallic acid, paeoniflorin and albiflorin was established by partial least squares (PLS). ResultsCorrection model correlation coefficient (R), corrected mean square error deviation (RMSEC) and mean square error deviation of internal validation (RMSEP) of gallic acid, paeoniflorin and albiflorin in Baishao were 0.99132, 0.0055 and 0.00896; 0.99869, 0.0266 and 0.10900; 0.97052, 0.0349 and 0.04210 respectively. Correlation coefficients between predicted value and real value of Baishao reached 0.9404, 0.9412 and 0.9446 by external verification. ConclusionThe method, accurate, rapid and simple, could realize the rapid analysis of the samples in large scales.

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备注/Memo

备注/Memo:
刘高宏（1974—），男，副主任中药师。研究方向：中药材鉴别及炮制研究。2019年全国中医药特色技术传承人才培训项目（T20194828003）。
更新日期/Last Update: 2022-08-09