[1]张如,魏江存,马家宝,等.大黄的炮制对生大黄、熟大黄5种游离蒽醌含量的影响测定[J].西部中医药,2021,34(12):25-31.[doi:10.12174/j.issn.2096-9600.2021.12.07]
 ZHANG Ru,WEI Jiangcun,MA Jiabao,et al.Processing of Dahuang and Determination of Five Free Anthraquinones in Crude and Processed Dahuang[J].Western Journal of Traditional Chinese Medicine,2021,34(12):25-31.[doi:10.12174/j.issn.2096-9600.2021.12.07]
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大黄的炮制对生大黄、熟大黄5种游离蒽醌含量的影响测定
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
34
期数:
2021年12期
页码:
25-31
栏目:
质量分析
出版日期:
2021-12-15

文章信息/Info

Title:
Processing of Dahuang and Determination of Five Free Anthraquinones in Crude and Processed Dahuang
作者:
张如1 魏江存2 马家宝3 杨正腾3 王成龙2
1.广西壮族自治区妇幼保健院,广西 南宁 530003
2.广西国际壮医医院
3.广西中医药大学第一附属医院
Author(s):
ZHANG Ru1 WEI Jiangcun2 MA Jiabao3 YANG Zhengteng3 WANG Chenglong2
1.Maternity and Child Health Care Hospital of Guangxi Zhuang Autonomous Region, Nanning 530003, China
2.Guangxi International Zhuang Medicine Hospital
3.The First Affiliated Hospital of Guangxi University of Chinese Medicine
关键词:
大黄游离蒽醌炮制高效液相色谱法
Keywords:
free anthraquinonesprocessingHPLC
分类号:
R283
DOI:
10.12174/j.issn.2096-9600.2021.12.07
文献标志码:
A
摘要:
目的采用高效液相色谱法(high performance liquid chromatography,HPLC)测定生、熟大黄中的5种游离蒽醌的含量及差异,探讨中药炮制前后物质基础的变化规律。 方法使用色谱柱为Thermo BDS HYPERSII C18柱(4.6 mm×250 mm,5 μm),以甲醇-0.1%磷酸(85∶15)水溶液为流动相,检测波长为256 nm,流速1.0 mL/min,柱温30℃,进样量10 μL。 结果芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚分别在0.0207~0.414(R2=0.9999)、0.1721~3.442(R2=0.9996)、0.0203~0.406(R2=0.9999)、0.148~2.96(R2=0.9998)、0.164~3.28(R2=0.9993)的线性范围内呈良好线性关系;生大黄经炮制后,芦荟大黄素含量下降约8.26%,大黄酸下降约9.21%;而大黄素含量增加约16.44%,大黄酚增加约16.12%,大黄素甲醚增加约20.01%,说明炮制工艺对大黄各成分影响不同。 结论大黄炮制后,其5种游离蒽醌的含量受到不同程度的影响,该方法可作为大黄及其不同炮制品的质量控制方法。
Abstract:
ObjectiveTo determine the contents and differences of five free anthraquinones in crude and processed Dahuang (Chinese rhubarb) using HPLC, and discuss the rules of material basis before and after the processing. MethodsThermo BDS HYPERSII C18 Column (4.6mm×250mm, 5μm) was used as the chroma-tographic column, methanol-0.1% phosphoric acid (85:15) aqueous solution as the mobile phase, detection wavelength was 256nm, flow rate 1.0mL/min, column temperature 30℃, sample size 10μL. ResultsAloe emodin, rhein, emodin, chrysophanol, emodin methyl ether showed better linear relationship in the range between 0.0207 and 0.414 (R2=0.9999), 0.1721 and 3.442 (R2=0.9996), 0.0203 and 0.406 (R2=0.9999), 0.148 and 2.96 (R2=0.9998), 0.164 and 3.28 (R2=0.9993) respectively; after processing, the contents of aloe emodin decreased by about 8.26%, the contents of rhein decreased by about 9.21%; while the contents of emodin increased by around 16.44%, the contents of chrysophanol by about 16.12% and emodin methyl ether by about 20.01%, which demo-nstrated that the processing showed different influences on all the ingredients of Dahuang. ConclusionAfter processing, the contents of five free anthraquinones are affected to varying degrees, and the method could be used for quality control of Dahuang and its processed products.

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备注/Memo

备注/Memo:
张如(1984—),女,副主任药师。研究方向:中药饮片、中成药处方调配核对工作以及中药质量标准研究。国家自然科学基金(81360524);2019年度广西高校中青年教师科研基础能力提升项目(2019KY0341);广西中医药大学青年基金项目(2019QN036);广西自然科学基金(2014GXNSFAA118208);国家中医药管理局科研三级实验室中(壮)药化学与质量分析实验室[国学基金中医药发2009(21)号)]。
更新日期/Last Update: 2021-12-15