[1]李美和,党慧敏,吴晓玲,等.白藜芦醇对人胎盘滋养细胞氧化应激损伤的影响及机制研究[J].西部中医药,2022,35(02):1-7.[doi:10.12174/j.issn.2096-9600.2022.02.01]
 LI Meihe,DANG Huimin,WU Xiaoling,et al.Effect of Resveratrol on Oxidative Stress Injury of Human Placental Trophoblast and Its Mechanism[J].Western Journal of Traditional Chinese Medicine,2022,35(02):1-7.[doi:10.12174/j.issn.2096-9600.2022.02.01]
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白藜芦醇对人胎盘滋养细胞氧化应激损伤的影响及机制研究
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
35
期数:
2022年02期
页码:
1-7
栏目:
药理研究
出版日期:
2022-02-15

文章信息/Info

Title:
Effect of Resveratrol on Oxidative Stress Injury of Human Placental Trophoblast and Its Mechanism
作者:
李美和 党慧敏 吴晓玲 刘艳巧 刘润侠 安鹏
西安交通大学第二附属医院(西北医院)
Author(s):
LI Meihe DANG Huimin WU Xiaoling LIU Yanqiao LIU Runxia AN Peng
The Second Affiliated Hospital of Xi′an Jiaotong University (Xibei Hospital), Xi′an 710000, China
关键词:
氧化应激白藜芦醇HTR-8/SVneo细胞凋亡
Keywords:
oxidative stressresveratrolHTR-8/SVneocellular apoptosis
分类号:
R285
DOI:
10.12174/j.issn.2096-9600.2022.02.01
文献标志码:
A
摘要:
目的探讨白藜芦醇(resveratrol,RES)对人胎盘滋养细胞HTR-8/SVneo的抗氧化应激损伤作用。 方法应用过氧化氢(hydrogen peroxide,H2O2)诱导建立人胎盘滋养细胞HTR-8/SVneo氧化应激模型,此模型加入不同浓度RES(10、50、100、200 μmol/L),作用8 h。观察各组细胞形态学改变,采用CCK-8测定细胞存活率,流式细胞仪检测细胞内活性氧簇(reactive oxygen species,ROS)水平及细胞凋亡率;应用分光光度法测定各组细胞匀浆中超氧化物歧化酶(superoxide dismutase,SOD)水平;应用酶联免疫吸附测定(ELISA)法检测细胞中半胱氨酸蛋白酶3(cysteinyl aspartate specific proteinase 3,caspase-3)及B细胞淋巴瘤/白血病2(B cell lymphoma/leukemia-2,Bcl-2)水平。 结果与空白对照组比较,模型组细胞出现皱缩、间隙增宽,有些呈片状坏死脱落;细胞存活率、细胞SOD水平明显下降(P<0.01);细胞凋亡率,细胞ROS、Caspase-3、Bcl-2水平均明显升高(P<0.01)。给予不同浓度RES(10、50、100、200 μmol/L)作用于人胎盘滋养细胞氧化应激模型8 h后,与模型组比较,RES 50 μmol/L组可明显改善氧化应激造成的细胞形态学改变,增加细胞存活率、细胞SOD水平(P<0.01);降低细胞凋亡率及细胞ROS、caspase-3、Bcl-2水平(P<0.01)。 结论RES对H2O2诱导产生氧化应激损伤的人胎盘滋养细胞HTR-8/SVneo具有保护作用,其作用机制是通过抗氧化及抗凋亡的途径。
Abstract:
ObjectiveTo explore the effects of RES against oxidative stress injury in human placental trophoblast HTR-8/SVneo. MethodsH2O2 was applied to induce and establish oxidative stress models of human placental trophoblast HTR-8/SVneo, different concentrations of RES (10,50,100 and 200 μmol/L)were added to the models for eight hours. To observe cell morphological changes in different groups, to detect cellular survival rate using CCK-8; to measure the levels of ROS and cellular apoptosis rate using flow cytometry, to detect the levels of SOD in cell homogenates using spectrophotometry; to detect the levels of caspase-3 and Bcl-2 in the cells using ELISA method. ResultsCompared with blank control group, the cells shrank, the gap widened, and some were necrotic and exfoliated in the model group; cellular survival rate and the levels of cellular SOD lowered notably (P<0.01); cellular apoptosis rate, the levels of cellular ROS, caspase-3 and Bcl-2 increased notably (P<0.01). After different concentrations of RES (10, 50, 100 and 200 μmol/L) acting on oxidative stress models of human placental trophoblast HTR-8/SVneo for eight hours, compared with the model group, RES50 μmol/L group could notably improve cell morphological changes induced by oxidative stress, increase cellular survival rate and the levels of cellular SOD (P<0.01); decreased cellular apoptosis rate, the levels of cellular ROS, caspase-3 and Bcl-2 (P<0.01). ConclusionRES could protect H2O2-induced oxidative stress damages in human placental trophoblast HTR-8/SVneo cells, and its mechanism might be through antioxidant and anti-apoptotic pathways.

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备注/Memo

备注/Memo:
李美和(1992—),女,博士学位。研究方向:复发性流产、排卵障碍、不孕症的诊治。国家自然科学基金青年科学基金(81703797)。
更新日期/Last Update: 2022-06-22