[1]谭本仁,黄义纯,陈华龙,等.高效液相色谱法测定复方黄蜀葵花喷雾剂中金丝桃苷含量[J].西部中医药,2022,35(07):40-42.[doi:10.12174/j.issn.2096-9600.2022.07.10]
 TAN Benren,HUANG Yichun,CHEN Hualong,et al.Contents of Hyperin in Compound Huangshu Kuihua Spray Determined by HPLC[J].Western Journal of Traditional Chinese Medicine,2022,35(07):40-42.[doi:10.12174/j.issn.2096-9600.2022.07.10]
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高效液相色谱法测定复方黄蜀葵花喷雾剂中金丝桃苷含量
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
35
期数:
2022年07期
页码:
40-42
栏目:
药理研究
出版日期:
2022-07-15

文章信息/Info

Title:
Contents of Hyperin in Compound Huangshu Kuihua Spray Determined by HPLC
作者:
谭本仁1 黄义纯2 陈华龙2 郑锦坤1 徐晓梅1
1.汕头大学医学院附属粤北人民医院,广东 韶关 512026
2.韶关市食品药品检验所
Author(s):
TAN Benren1 HUANG Yichun2 CHEN Hualong2 ZHENG Jinkun1 XU Xiaomei1
1.Yuebei People's Hospital Affiliated to Shantou University Medical College, Shaoguan 512026, China
2.Shaoguan City Institute for Food and Drug Control
关键词:
复方黄蜀葵花喷雾剂高效液相色谱法含量测定金丝桃苷
Keywords:
compound sprayHPLCcontent determinationhyperin
分类号:
R284.1
DOI:
10.12174/j.issn.2096-9600.2022.07.10
文献标志码:
A
摘要:
目的建立复方黄蜀葵花喷雾剂中金丝桃苷含量的测定方法。 方法采用高效液相色谱法(high performance liquid chromatography,HPLC),色谱柱为KromasiL 100-5-C18(5 μm,250 mm×4.6 mm),以乙腈和2%磷酸盐缓冲溶液为流动相,梯度洗脱,检测波长为401 nm;柱温30℃;流速1 mL/min。 结果金丝桃苷在10.26~205.17 μg/mL范围内呈良好的线性关系;平均回收率为96.9%,RSD=0.9%(n=6)。 结论该方法简捷、快速、可靠,可用于复方黄蜀葵花喷雾剂的质量控制。
Abstract:
ObjectiveTo establish the content determination methods for hyperin in compound Huangshu Kuihua spray. MethodsHPLC was used, chromatographic column: KromasiL 100-5-C18(5 μm, 250 mm×4.6 mm), acetonitrile and 2% phosphate buffer solution were chosen as the mobile phase, gradient elution, detection wavelength was 401nm; column temperature 30 ℃; flow rate was 1mL·min-1. ResultsHyperin showed good linear relationship between 10.26 and 205.17 μg/mL; average recovery rate was 96.9%, RSD=0.9% (n=6). ConclusionThe method, simple, rapid and reliable, could be used for quality control of compound Huangshu Kuihua spray.

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备注/Memo

备注/Memo:
谭本仁(1973—),男,主任药师。研究方向:医院药学。广东省中医药局科研项目(20212228);韶关市科技计划项目(200812114531498);韶关市卫生健康科研项目(Y20233)。
更新日期/Last Update: 2022-07-15