[1]李锦,华海应,高华强,等.补肾健脾化瘀中药对难治/复发再生障碍性贫血患者骨髓CD3+T淋巴细胞内p-mTOR、p-S6、IFN-γ表达的影响[J].西部中医药,2022,35(09):4-9.[doi:10.12174/j.issn.2096-9600.2022.09.02]
　LI Jin,HUA Haiying,GAO Huaqiang,et al.Effects of Medicine of Tonifying-kidney Invigorating-spleen Eliminating-stasis on the Expressions of p-mTOR, p-S6 and IFN-γ in CD3+ T Lymphocytes in Patients with Refractory/Recurrent Aplastic Anemia[J].Western Journal of Traditional Chinese Medicine,2022,35(09):4-9.[doi:10.12174/j.issn.2096-9600.2022.09.02]
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补肾健脾化瘀中药对难治/复发再生障碍性贫血患者骨髓CD3+T淋巴细胞内p-mTOR、p-S6、IFN-γ表达的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
35
期数:
2022年09期
页码:
4-9
栏目:
临床论著
出版日期:
2022-09-15

文章信息/Info

Title:
Effects of Medicine of Tonifying-kidney Invigorating-spleen Eliminating-stasis on the Expressions of p-mTOR, p-S6 and IFN-γ in CD3+ T Lymphocytes in Patients with Refractory/Recurrent Aplastic Anemia
作者:
李锦1, 华海应1, 高华强1, 朱文艳1, 周晔1, 王志清1, 张艳1, 何广胜2
1.江南大学附属医院血液科，江苏 无锡 214041
2.南京医科大学第一附属医院血液科
Author(s):
LI Jin1, HUA Haiying1, GAO Huaqiang1, ZHU Wenyan1, ZHOU Ye1, WANG Zhiqing1, ZHANG Yan1, HE Guangsheng2
1.Hematology Department, Affiliated Hospital of Jiangnan University, Wuxi 214041, China
2.Hematology Department, First Affiliated Hospital of Nanjing Medical University
关键词:
再生障碍性贫血; 难治/复发; 磷酸化哺乳动物雷帕霉素靶蛋白; 磷酸化核糖体蛋白S6; 干扰素; 补肾健脾化瘀中药
Keywords:
aplastic anemia; refractory/recurrent; p-mTOR; p-S6; IFN-medicine of tonifying-kidney invigorating-spleen eliminating-stasis
分类号:
R255.7
DOI:
10.12174/j.issn.2096-9600.2022.09.02
摘要:
目的探讨补肾健脾化瘀中药对难治/复发再生障碍性贫血（aplastic anemia，AA）患者骨髓CD3+T淋巴细胞内磷酸化哺乳动物雷帕霉素靶蛋白（phosphorylated mammalian target of rapamycin，p-mTOR）、磷酸化核糖体蛋白S6(phosphorylated ribosomal protein S6，p-S6)及干扰素γ（interferon-γ，IFN-γ）的影响，解析其治疗难治/复发AA的机制。 方法采集初发AA 13例、难治/复发AA 15例及对照组14例研究者的骨髓液，分别加入补肾健脾化瘀中药、雷帕霉素进行孵育，运用流式细胞术检测药物处理前后各组CD3+T淋巴细胞胞质内p-mTOR、p-S6与IFN-γ表达水平。 结果药物处理前，复发/难治AA患者CD3+T淋巴细胞p-mTOR、p-S6、IFN-γ表达均高于初发AA与对照组（P＜0.01）；初发AA患者与对照组比较p-mTOR、p-S6表达差异无统计学意义（P＞0.05）；初发AA患者IFN-γ表达高于对照组（P＜0.01）。药物处理后，复发/难治AA组中，补肾健脾化瘀中药及雷帕霉素均能降低CD3+T淋巴细胞p-mTOR、p-S6与IFN-γ表达（P＜0.01），雷帕霉素与补肾健脾化瘀中药组比较各指标水平差异无统计学意义（P＞0.05）；初发AA与对照组中，雷帕霉素、补肾健脾化瘀中药不能明显降低CD3+T淋巴细胞p-mTOR、p-S6表达（P＞0.05）；初发AA组中，雷帕霉素、补肾健脾化瘀中药能降低CD3+T淋巴细胞IFN-γ表达（P＜0.01）；对照组中，雷帕霉素、补肾健脾化瘀中药不能降低CD3+T淋巴细胞IFN-γ表达（P＞0.05）。 结论补肾健脾化瘀中药通过抑制难治/复发AA患者CD3+T淋巴细胞内p-mTOR、p-S6、IFN-γ表达，进而抑制T淋巴细胞的异常激活，改善骨髓造血功能。
Abstract:
ObjectiveTo analyze the mechanism of medicine of tonifying-kidney invigorating-spleen eliminating-stasis in the treatment of refractory/recurrent aplastic anemia (AA) by discussing the effects of the medicine on the expressions of p-mTOR, p-S6 and IFN-γ in CD3+T lymphocytes in marrow of refractory/recurrent AA patients. MethodsAfter collecting the marrow fluid from 13 cases of incipient AA, 15 cases of refractory/recurrent AA and 14 cases in the control group, medicine of tonifying-kidney invigorating-spleen eliminating-stasis and rapamycin were respectively added to perform the incubation. Flow cytometry was used to detect the expressions of p-mTOR, p-S6 and IFN-γ in CD3+T lymphocytes in different groups before and after processing with medicine. ResultsBefore medication, the expressions of p-mTOR, p-S6 and IFN-γ in CD3+T lymphocytes in refractory/recurrent AA were higher than these of incipient AA and the control group (P<0.01); the difference had no statistical meaning in the expressions of p-mTOR and p-S6 between the patients with incipient AA and the control group (P>0.05); the expressions of IFN-γ in patients with incipient AA were higher than these of the control group (P<0.01). After medicating, in group of refractory/recurrent AA, medicine of tonifying-kidney invigorating-spleen eliminating-stasis and rapamycin could reduce the expressions of p-mTOR, p-S6 and IFN-γ in CD3+T lymphocytes (P<0.01); while in the group of incipient AA and the control group, rapamycin and herbs could not obviously lower the expressions of p-mTOR and p-S6 in CD3+T lymphocytes (P>0.05); in the group of incipient AA, both of the medicine could reduce the expression of IFN-γ in CD3+T lymphocytes (P<0.01); in the control group, both of the medicine could not obviously lower the expression of IFN-γ in CD3+T lymphocytes (P>0.05). ConclusionMedicine of tonifying-kidney invigorating-spleen eliminating-stasis could inhibit the abnormal activation of T lymphocyte and improve bone marrow hematopoietic function through restraining the expressions of p-mTOR, p-S6 and IFN-γ in CD3+T lymphocytes in patients suffering refractory/recurrent AA.

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备注/Memo

备注/Memo:
李锦（1988—），女，硕士学位。研究方向：骨髓衰竭性疾病的基础与临床。国家中医药管理局项目（201407001-4）；无锡市医院管理中心重点研究资助项目（YGZXZ1512）。
更新日期/Last Update: 2022-10-08