[1]任慧,崔小敏,胡静,等.一测多评法对金铃子散中来源于延胡索的5个生物碱成分含量的同时测定[J].西部中医药,2022,35(11):73-77.[doi:10.12174/j.issn.2096-9600.2022.11.13]
　REN Hui,CUI Xiaomin,HU Jing,et al.Simultaneous Determination of Five Alkaloids from Corydalis Rhizoma in Jinlingzi Powder by QAMS[J].Western Journal of Traditional Chinese Medicine,2022,35(11):73-77.[doi:10.12174/j.issn.2096-9600.2022.11.13]
点击复制

一测多评法对金铃子散中来源于延胡索的5个生物碱成分含量的同时测定
分享到：

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
35
期数:
2022年11期
页码:
73-77
栏目:
出版日期:
2022-11-15

文章信息/Info

Title:
Simultaneous Determination of Five Alkaloids from Corydalis Rhizoma in Jinlingzi Powder by QAMS
作者:
任慧, 崔小敏, 胡静, 刘小妹, 陈志永
陕西省中医药研究院，陕西 西安 710003
Author(s):
REN Hui, CUI Xiaomin, HU Jing, LIU Xiaomei, CHEN Zhiyong
Shaanxi Academy of Traditional Chinese Medicine, Xi'an 710003, China
关键词:
金铃子散; 一测多评法; 高效液相色谱法; 生物碱
Keywords:
powder; QAMS; HPLC; alkaloid
分类号:
R284.1
DOI:
10.12174/j.issn.2096-9600.2022.11.13
文献标志码:
A
摘要:
目的建立同时测定金铃子散中延胡索乙素、黄连碱、紫堇碱、巴马汀和去氢紫堇碱含量的一测多评法（quantitative analysis of multi-components with single marker，QAMS），验证该方法在金铃子散质量控制应用中的科学性和可行性。 方法采用Agilent 5 TC-C18色谱柱（250 mm×4.6 mm，5 μm），以乙腈-0.1%磷酸水溶液为流动相，梯度洗脱，柱温30 ℃，流速1.0 mL/min，检测波长280 nm；以延胡索乙素为内参物，建立该成分与其他4种成分的相对校正因子，以相对校正因子计算各成分含量，同时借助余弦相似度评价一测多评法计算结果和外标法实测值的差异。 结果5种成分在各自范围内线性关系良好，平均加样回收率在96.79%～102.41%之间，RSD在0.45%～2.67%之间；建立的各相对校正因子耐用性良好，一测多评法和外标法含量测定结果无显著差异。 结论利用相对校正因子对金铃子散中5种生物碱含量测定可行，所建立的一测多评法可用于金铃子散的质量评价。
Abstract:
ObjectiveTo establish a method of QAMS for simultaneous determination of tetrahydropalmatine, coptisine, corydaline, palmatine, dehydrocorydaline contained in Jinlingzi powder, so as to verify the scientificity and feasibility of the method applied in the quality control of Jinlingzi powder. MethodsAgilent 5 TC-C18 chromatographic column (250 mm×4.6 mm, 5 μm) was adopted, acetonitrile-0.1% phosphoric acid aqueous solutions as the mobile phase, gradient elution, column temperature 30 ℃, flow rate 1.0 mL/min, detection wavelength 280 nm; by taking tetrahydropalmatine as the internal reference, relative correction factor between the component and other four components were established, and the contents of different components were calculated by relative correction factor, in the meantime, to assess the difference beween the calculated results of QAMS and the measured value of external reference method by using cosine similarity. ResultsFive components presented good linearity in their own ranges, average recovery rates were between 96.79% and 102.41%, RSD was in the range from 0.45% to 2.67%; The established relative correction factors have good durability, no significant difference has been found in the results of QAMS and the ones of external reference method. ConclusionIt is feasible to determine the contents of five alkaloids in Jinlingzi powder, QAMS easblished could be applied to quality evaluation of Jinlingzi powder.

相似文献/References:

[1]刘鸿雁,吴晶,刘丽娜,等.一测多评法同时测定苍柏祛痛胶囊中盐酸小檗碱和盐酸黄柏碱的含量[J].西部中医药,2015,28(11):23.
[2]张生杰,王丽,田志梅,等.基于一测多评法测定白芍中6种成分含量[J].西部中医药,2022,35(03):44.[doi:10.12174/j.issn.2096-9600.2022.03.10]
　ZHANG Shengjie,WANG Li,TIAN Zhimei,et al.Content Determination of Six Components in Baishao by Quantitative Analysis of Multi-components by Single-marker[J].Western Journal of Traditional Chinese Medicine,2022,35(11):44.[doi:10.12174/j.issn.2096-9600.2022.03.10]
[3]李梦华,田丽颖.基于网络药理学探讨金铃子散改善痛经的作用机制[J].西部中医药,2022,35(10):42.[doi:10.12174/j.issn.2096-9600.2022.10.09]
　LI Menghua,TIAN Liying.The Effects of Jinlingzi Powder in Improving Dysmenorrhea on the Basis of Network Pharmacology[J].Western Journal of Traditional Chinese Medicine,2022,35(11):42.[doi:10.12174/j.issn.2096-9600.2022.10.09]

备注/Memo

备注/Memo:
任慧（1989—），女，博士学位，副研究员。研究方向：中药质量控制与活性成分。国家自然科学基金面上项目（81973419）；陕西省重点研发计划一般项目（2018SF-302，2019SF-302）；西安市科技计划项目［2019115613YX011SF044（13），201805103YX11SF37（17）］；陕西省中医药管理局中医药科研课题（JCMS007）。
更新日期/Last Update: 2022-11-15