[1]许瑞,毕艳平,邹国良,等.梓醇对2型糖尿病大鼠心肌纤维化及心肌细胞形态学的影响[J].西部中医药,2024,37(01):1-4.[doi:10.12174/j.issn.2096-9600.2024.01.01]
　XU Rui,BI Yanping,ZOU Guoliang,et al.Effects of Catalpol on Myocardial Fibrosis and Myocardial Cells Morphology in Rats with Type 2 Diabetes Mellitus[J].Western Journal of Traditional Chinese Medicine,2024,37(01):1-4.[doi:10.12174/j.issn.2096-9600.2024.01.01]
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梓醇对2型糖尿病大鼠心肌纤维化及心肌细胞形态学的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
37
期数:
2024年01期
页码:
1-4
栏目:
基础研究
出版日期:
2024-01-15

文章信息/Info

Title:
Effects of Catalpol on Myocardial Fibrosis and Myocardial Cells Morphology in Rats with Type 2 Diabetes Mellitus
作者:
许瑞1,2, 毕艳平2, 邹国良3, 王研2, 史建平2, 张艳1
1.辽宁中医药大学，辽宁 沈阳 110032
2.吉林中西医结合医院，吉林 吉林 132012
3.黑龙江中医药大学附属第一医院，黑龙江 哈尔滨 150040
Author(s):
XU Rui1,2, BI Yanping2, ZOU Guoliang3, WANG Yan2, SHI Jianping2, ZHANG Yan1
1.Liaoning University of Traditional Chinese Medicine, Liaoning 110032, China
2.Jilin Integrated Traditional Chinese and Western Medicine Hospital, Jilin 132012, China
3.First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, China
关键词:
2型糖尿病; 心肌纤维化; 心肌细胞形态学; 梓醇
Keywords:
type 2 diabetes mellitus; myocardial fibrosis; myocardial cells morphology; catalpol
分类号:
R285.5
DOI:
10.12174/j.issn.2096-9600.2024.01.01
文献标志码:
A
摘要:
目的探讨梓醇对2型糖尿病（type 2 diabetes mellitus，T2DM）大鼠心肌纤维化及形态学的影响。 方法采用随机数字表法将90只SPF级雄性Wistar大鼠随机分为正常组10只和实验组80只。实验组大鼠高脂高糖饲料喂养8周后，腹腔注射链脲佐菌素（streptozotocin，STZ）溶液15 mg/kg制备T2DM模型。将成模大鼠根据空腹血糖（fasting plasma glucose，FPG）、体质量随机分为模型组、二甲双胍组与梓醇低、中、高剂量组。二甲双胍组灌胃二甲双胍混悬液90 mg/kg，梓醇低、中、高剂量组分别灌胃梓醇2.5、5、10 mg/kg，正常组和模型组灌胃等体积生理盐水，每日1次，均灌胃12周。末次给药后，测定各组大鼠FPG；免疫组化法检测心肌组织转化生长因子β1（transforming growth factor-β1，TGF-β1）表达；HE染色观察心肌组织结构情况。 结果与模型组比较，梓醇高、中、低剂量组大鼠FPG及心肌TGF-β1降低（P＜0.05）；梓醇高剂量组与正常组比较，心肌纤维、胞质、胶原纤维、细胞核均无显著差异。模型组与梓醇低剂量组心肌纤维排列紊乱或不规整，部分溶解、断裂，甚至呈小片状分布，胞质染色不均匀，中度或轻度肿胀；部分细胞核变形，固缩，甚至碎裂消失；心肌间隙显著增宽，可见大量或少量炎性细胞或成纤维细胞。二甲双胍组与梓醇中剂量组心肌纤维排列略规整，心肌细胞核极少部分变形；心肌间隙略增宽，炎性细胞较少。 结论梓醇可降低T2DM大鼠FPG与心肌纤维化水平，可能为保护T2DM大鼠心肌细胞的有效手段。
Abstract:
ObjectiveTo study the effects of catalpol on myocardial fibrosis and morphology in the rats with type 2 diabetes mellitus (T2DM). MethodsSPF grade healthy male Wistar rats were randomized into 10 ones in the normal group and 80 ones in the experiment group using random number table method.The rats in the experimental group accepted peritoneal injection of STZ solution,15 mg/kg, to prepare T2DM models after feeding with high-fat and high-sugar fodder for eight weeks. Rat models were divided into the model group，melbine (DMBG) group, low, moderate and high dose groups of catalpol according to FPG and body mass. DMBG group was drenched with DMBG suspension, 90 mg/kg, low, moderate and high dose groups of catalpol accepted intragastric administration of catalopl at 2.5, 5 and 10 mg/kg respectively, the normal group and the model group accepted equivalent volume of physiological saline by gavage, once each day, drenched for 12 weeks. Since the last dose, to detect FPG, immunohistochemical method was applied to detect the expressions of TGF-β1 in myocardial tissue; HE staining to observe the morphology of myocardial cells. ResultsCompared with the model group, the levels of FPG and myocardial TGF-β1 reduced in high, moderate and low dose groups of catalpol (P<0.05); there was no significant difference in myocardial fiber, cytoplasm, collagen fibers and nucleus when high dose group was compared with the normal group. The myocardial fibers were disordered or irregular, partly dissolved, broken, even distributed in small flakes, the cytoplasm was stained unevenly, with moderate or slight swelling.Some nuclei were deformed, showing pyknosis, even disintegrated in the model group and low dose group.The myocardial gap widened significantly, large or small numbers of inflammatory cells or fibroblasts could be seen.the arrangement of myocardial fibers was slightly regular, and the nucleus of myocardial cells was rarely deformed in DMBG group and moderate dose group; myocardial gap is slightly widened, with fewer inflammatory cells. ConclusionCatalpol could reduce the levels of FPG and myocardial fibrosis in T2DM rats, and it may be an effective way of protecting myocardial cells in T2DM rats.

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备注/Memo

备注/Memo:
许瑞（1988—），男，在读博士研究生，主治医师。研究方向：心血管疾病的中西医结合治疗。国家自然科学基金面上项目（82174241）；吉林省中医药管理局项目（2017276）；吉林省吉林市科技发展计划项目（201737111）。
更新日期/Last Update: 2024-01-15