[1]刘曼曼,冯珍凤,胡春平,等.滋膵降糖方介导Fetuin B-AMPK/ACC通路对高脂诱导肥胖小鼠肝脏胰岛素抵抗的调控机制研究[J].西部中医药,2024,37(02):14-19.[doi:10.12174/j.issn.2096-9600.2024.02.04]
　LIU Manman,FENG Zhenfeng,HU Chunping,et al.Study on Regulatory Mechanism of Fetuin B-AMPK/ACC Pathway Mediated by Zicui Jiangtang Prescription on Insulin Resistance in Liver of High-fat-induced Obese Mice[J].Western Journal of Traditional Chinese Medicine,2024,37(02):14-19.[doi:10.12174/j.issn.2096-9600.2024.02.04]
点击复制

滋膵降糖方介导Fetuin B-AMPK/ACC通路对高脂诱导肥胖小鼠肝脏胰岛素抵抗的调控机制研究
分享到：

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
37
期数:
2024年02期
页码:
14-19
栏目:
基础研究
出版日期:
2024-02-15

文章信息/Info

Title:
Study on Regulatory Mechanism of Fetuin B-AMPK/ACC Pathway Mediated by Zicui Jiangtang Prescription on Insulin Resistance in Liver of High-fat-induced Obese Mice
作者:
刘曼曼1, 冯珍凤1, 胡春平1, 陈见纺1, 沈怡华1, 高俊凤2, 严军1
1.上海市嘉定区中医医院，上海 201899
2.上海中医药大学研究生院，上海 201203
Author(s):
LIU Manman1, FENG Zhenfeng1, HU Chunping1, CHEN Jianfang1, SHEN Yihua1, GAO Junfeng2, YAN Jun1
1.Shanghai City Jiading District Hospital of Traditional Chinese Medicine, Shanghai 201899, China
2.Graduate School, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
关键词:
滋膵降糖方; Fetuin B; AMPK/ACC; 胰岛素抵抗
Keywords:
prescription; Fetuin B; AMPK/ACC; insulin resistance
分类号:
R587.1
DOI:
10.12174/j.issn.2096-9600.2024.02.04
文献标志码:
A
摘要:
目的探讨滋膵降糖方通过Fetuin B-AMPK/ACC通路改善肥胖C57BL/6J小鼠胰岛素抵抗的机制。 方法高脂饮食喂养C57BL/6J小鼠，构建胰岛素抵抗模型，随机分为模型组、滋膵降糖方组、二甲双胍组、联合组（滋膵降糖方+二甲双胍），正常饮食小鼠为空白对照组。观察各组小鼠体质量、肝脏质量、葡萄糖耐量、胰岛素抵抗指数，肝脏Fetuin B、AMPK、ACC mRNA及肝脏Fetuin B、AMPKα1、ACC、P-AMPKαT183/T172、P-ACCS79蛋白表达。 结果滋膵降糖方可改善小鼠葡萄糖耐量，减小曲线下面积，降低胰岛素抵抗指数，下调肝脏质量和肝体比，降低肝脏Fetuin B、ACC mRNA和Fetuin B蛋白表达，上调AMPK mRNA和P-AMPKαT183/T172/AMPKα1、P-ACCs79/ACC蛋白水平。 结论滋膵降糖方可能通过Fetuin B-AMPK/ACC信号通路减轻肥胖小鼠肝脏胰岛素抵抗。
Abstract:
ObjectiveTo explore the mechanism of improving insulin resistance in obese C57BL/6J mice via Fetuin B-AMPK/ACC pathway medicated by Zicui Jiangtang prescription. MethodsThe models with insulin resistance (IR) were established by feeding C57BL/6J mice high-fat diet, and they were randomly allocated to the model group, Zicui Jiangtang prescription group, metformin group, the combination group (Zicui Jiangtang prescription+metformin), the mice fed normal diet were chosen as blank control group. To observe the mice's body mass, liver mass, glucose tolerance, insulin resistance index (IRI), the expressions of liver Fetuin B, AMPK, ACC mRNA, liver Fetuin B, AMPKα1, ACC, P-AMPKαT183/T172 and P-ACCS79 protein. ResultsZicui Jiangtang prescrip-tion could improve the mice′ glucose tolerance, reduce area under the curve, lower IRI, liver mass and hepatocrit, cut down the expressions of liver Fetuin B, ACC mRNA and Fetuin B protein, up regulate the levels of AMPK mRNA, P-AMPKαT183/T172 and P-ACCS79/ACC protein. ConclusionZicui Jiangtang prescription could alleviate IR of liver in obese mice possibly via Fetuin B-AMPK/ACC signaling pathway.

相似文献/References:

[1]刘曼曼,沈怡华,冯珍凤,等.滋月萃降糖方对2型糖尿病合并非酒精性脂肪性肝病患者血清胎球蛋白B水平及糖脂代谢的影响[J].西部中医药,2023,36(03):1.[doi:10.12174/j.issn.2096-9600.2023.03.01]
　LIU Manman,SHEN Yihua,FENG Zhenfeng,et al.Influence of Nourishing-pancreas Lowering-glucose Prescription on the Levels of Serum Fetuin B, Sugar-fat Metabolism in Patients with T2DM and NAFLD[J].Western Journal of Traditional Chinese Medicine,2023,36(02):1.[doi:10.12174/j.issn.2096-9600.2023.03.01]

备注/Memo

备注/Memo:
刘曼曼（1987—），女，硕士学位，主治医师。研究方向：内分泌代谢性疾病的中医药防治。上海市嘉定区科委项目（JDKW-2019-W17）；上海市“十四五”中医特色专科项目（ZYTSZK1-10）；上海市嘉定区卫健委项目（2023-KY-ZYY-03）。
更新日期/Last Update: 2024-02-15