[1]高野,李凤岩,闫睿,等.甘草次酸对大肠癌HT-29细胞化疗增敏作用机制研究[J].西部中医药,2024,37(08):11-14.[doi:10.12174/j.issn.2096-9600.2024.08.03]
　GAO Ye,LI Fengyan,YAN Rui,et al.Chemotherapy Enhancement Effects of Glycyrrhetinic Acid on Human Colorectal Cancer HT-29 Cells[J].Western Journal of Traditional Chinese Medicine,2024,37(08):11-14.[doi:10.12174/j.issn.2096-9600.2024.08.03]
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甘草次酸对大肠癌HT-29细胞化疗增敏作用机制研究
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
37
期数:
2024年08期
页码:
11-14
栏目:
基础研究
出版日期:
2024-08-15

文章信息/Info

Title:
Chemotherapy Enhancement Effects of Glycyrrhetinic Acid on Human Colorectal Cancer HT-29 Cells
作者:
高野1, 李凤岩1, 闫睿1, 白剑2, 苗兰英2
1.朝阳市第二医院，辽宁 朝阳 122000
2.辽宁中医药大学实验动物中心，辽宁 沈阳 110847
Author(s):
GAO Ye1, LI Fengyan1, YAN Rui1, BAI Jian2, MIAO Lanying2
1.The Second Hospital of Chaoyang, Chaoyang 122000, China
2.Laboratory Animal Center, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China
关键词:
大肠癌; HT-29细胞; 核转录因子B; 凋亡; 甘草次酸
Keywords:
colorectal cancer; HT-29 cells; NF-B; apoptosis; glycyrrhetinic acid
分类号:
R285.5
DOI:
10.12174/j.issn.2096-9600.2024.08.03
文献标志码:
A
摘要:
目的研究甘草次酸增强奥沙利铂对大肠癌HT-29细胞化疗敏感性的作用机制。 方法将大肠癌HT-29细胞分为对照组、甘草次酸组（甘草次酸浓度为100 μmol/L）、联合组（100 μmol/L甘草次酸+25 mg/L奥沙利铂）和阳性对照组（奥沙利铂25 mg/L）。采用四氮唑蓝法检测各组HT-29细胞增殖率；通过Annexin V/PI双标流式细胞术检测各组HT-29细胞凋亡比例；通过蛋白质印迹法检测各组HT-29细胞中核转录因子κB（nuclear factor kappa B，NF-κB）、B淋巴细胞瘤2（B-cell lymphoma 2，Bcl-2）、激活型胱天蛋白酶3（caspase-3，CASP-3）蛋白表达。 结果与对照组相比，联合组和阳性对照组HT-29细胞增殖率降低（P＜0.05），联合组和阳性对照组凋亡细胞比例均升高（P＜0.05）；甘草次酸组、联合组和阳性对照组NF-κB、Bcl-2蛋白相对表达量均降低（P＜0.05），而激活型CASP-3蛋白表达升高（P＜0.05）。 结论甘草次酸可能通过调控NF-κB信号通路增强奥沙利铂对大肠癌HT-29细胞的化疗敏感性。
Abstract:
ObjectiveTo investigate the mechanism of glycyrrhetinic acid enhancing the chemosentivity of oxaliplatin to human colorectal cancer HT-29 cells. MethodsColorectal cancer HT-29 cells were allocated to the control group, glycyrrhetinic acid group (the concentrations of glycyrrhetinic acid: 100 μmol/L), the combination group (100 μmol/L of glycyrrhetinic acid+25 mg/L oxaliplatin) and positive control group (25 mg/L oxaliplatin). Methylthiazolyl tetrazolium(MTT) was adopted to detect the proliferation rate of HT29 cells in different groups; Annexin V/PI double labeling flow cytometry was applied to measure the apoptosis ratio of HT-29 cells in different groups; Western blotting method was used to detect the expressions of NF-κB, Bcl-2 and CASP-3 in HT-29 cells in all the groups. ResultsCompared with the control group, the proliferation rate of HT-29 cells was reduced in the combination group and positive control group (P<0.05), cellular apoptosis rate was raised in the combina-tion group and positive control group (P<0.05); the relative expressions of NF-κB and Bcl-2 proteins were lowered in glycyrrhetinic acid group, the combination group and positive control group (P<0.05), while the expressions of activated CASP-3 protein were increased (P<0.05). ConclusionGlycyrrhetinic acid could enhance the chemosentivity of oxaliplatin to human colorectal cancer HT-29 cells possibly through regulating NF-κB signaling pathway.

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备注/Memo

备注/Memo:
高野（1981—），男，副主任医师。研究方向：胃肠外科肿瘤的治疗。甘肃省科技计划项目（18JR2FA009）；中医药传承与创新“百千万”人才工程（岐黄工程）岐黄学者项目（国中医药人教〔2018〕12号）；国家中医药管理局全国中医学术流派传承工作室建设项目（LP28061）；甘肃中医药大学研究生创新基金项目（CY2020-03）。 辽宁省自然科学基金（2015020714）。
更新日期/Last Update: 2024-08-15