[1]蒋欣琪,卢涛,于国华,等.基于网络药理学探讨知柏地黄丸治疗高血压的作用机制[J].西部中医药,2025,38(05):38-45.[doi:10.12174/j.issn.2096-9600.2025.05.09]
　JIANG Xinqi,LU Tao,YU Guohua,et al.The Mechanism of Treatment of Hypertension with Zhibai Dihuang Pills Based on Network Pharmacology[J].Western Journal of Traditional Chinese Medicine,2025,38(05):38-45.[doi:10.12174/j.issn.2096-9600.2025.05.09]
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基于网络药理学探讨知柏地黄丸治疗高血压的作用机制()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
38
期数:
2025年05期
页码:
38-45
栏目:
二次研究
出版日期:
2025-05-15

文章信息/Info

Title:
The Mechanism of Treatment of Hypertension with Zhibai Dihuang Pills Based on Network Pharmacology
作者:
蒋欣琪1, 卢涛1, 于国华1, 董易杭1, 张金东1, 毛伯1, 文雅倩2, 张晓晴1,3
1.北京中医药大学，北京 100029
2.广州中医药大学，广东 广州 510006
3.清华大学天津高端装备研究院，天津 300308
Author(s):
JIANG Xinqi1, LU Tao1, YU Guohua1, DONG Yihang1, ZHANG Jindong1, MAO Boyan1, WEN Yaqian2, ZHANG Xiaoqing1,3
1.Beijing University of Chinese Medicine, Beijing 100029, China
2.Guangzhou University of Chinese Medicine, Guangzhou 510006, China
3.Tianjin Research Institute for Advanced Equipment, Tsinghua University, Tianjin 300308, China
关键词:
高血压; 知柏地黄丸; 网络药理学; 潜在靶点; 功能富集; 通路富集
Keywords:
hypertension; pills; network pharmacology; potential targets; functional enrichment; pathway enrichment
分类号:
R256.21
DOI:
10.12174/j.issn.2096-9600.2025.05.09
文献标志码:
A
摘要:
目的通过网络药理学方法探讨知柏地黄丸治疗高血压的潜在靶点及信号通路。 方法通过TCMSP、Swiss Target Prediction、DrugBank数据库，对中药知柏地黄丸中8味药物的主要有效成分进行检索，并预测其靶点。结合OMIM、GeneCards、DisGeNET、TTD数据库搜索高血压的相关靶点，将二者取交集。运用STRING数据库得到知柏地黄丸作用于高血压的PPI网络，然后使用Cytoscape对PPI网络进行拓扑分析得到关键靶点。最后基于R语言、Metascape软件及DAVID数据库进行基因本体论（gene ontology，GO）功能富集和京都基因与基因组百科全书（kyoto encyclopedia of genes and genomes，KEGG）通路富集。 结果共得到IL-6、VEGFA、PTGS2、CASP3、JUN等30个知柏地黄丸与高血压的关键相互作用靶点。GO功能富集分析显示，知柏地黄丸治疗高血压与腺苷酸环化酶激活肾上腺素能受体信号通路、葡萄糖稳态、类固醇代谢过程等相关。KEGG通路富集发现知柏地黄丸治疗高血压关联神经活性配体-受体相互作用、含血清素的神经突触、卵巢类固醇生成，缺氧诱导因子1（hypoxia-inducible factor-1，HIF-1）信号通路等多条通路。 结论知柏地黄丸降压的潜在生物学作用机制是通过调控神经活性配体-受体相互作用、含血清素的神经突触，HIF-1信号通路等发挥治疗作用，反映了知柏地黄丸多成分、多靶点，多环节协调治疗高血压的特点。
Abstract:
ObjectiveTo discuss the potential targets and signaling pathways of Zhibai Dihuang pills in the treatment of hypertension through network pharmacology. MethodsThe main active ingredients of eight herbs contained in Chinese patent drug Zhibai Dihuang pills were searched from TCMSP, Swiss Target Prediction and DrugBank, and the prediction of the targets were conducted. Meanwhile, hypertension-related targets were retrieved from OMIM, GeneCards, DisGeNET and TTD databases, to obtain the intersection from both. STRING database was applied to gain PPI network after treating hypertension with the medicine, the topology analysis of PPI network was carried out using Cytoscape so as to gain the key targets. Consequently, GO and KEGG were performed based on R language, Metascape software and DAVID databases. ResultsThere were 30 key interaction targets between Zhibai Dihuang pills and hypertension, covering IL-6, VEGFA, and PTGS2, CASP3 and JUN. GO functional enrichment analysis displayed that the treatment of hypertension with Zhibai Dihuang pills was related to adenylate cyclase - activating adrenergic receptor signaling pathway, glucose homeostasis and steroid metabolism. KEGG pathway enrichment indicated that Zhibai Dihuang pills in the treatment of hypertension was associated with many pathways such as neuroactive ligand-receptor interaction, serotonin-containing synapses, ovarian steroid generation and HIF-1 signaling pathway. ConclusionThe potential biological mechanism of Zhibai Dihuang pills in lowering blood pressure could exert therapeutic effects by regulating neuroactive ligand-receptor interaction, serotonin-containing synapses, ovarian steroid generation and HIF-1 signaling pathway, reflecting the characteristics of multi-component, multi-target and multi-link coordinated treatment of hypertension by Zhibai Dihuang Pills.

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备注/Memo

备注/Memo:
蒋欣琪（1998—），男，硕士学位。研究方向：中医药现代化和大数据分析。Email：13255185895@163.com。国家重点研发计划项目（2017YFC1703306）。
更新日期/Last Update: 2025-05-15