[1]李文娟,周婷,张杰,等.CB1R/BDNF调控异相睡眠剥夺诱发术后疼痛敏化的机制研究[J].西部中医药,2025,38(06):5-12.[doi:10.12174/j.issn.2096-9600.2025.06.02]
　LI Wenjuan,ZHOU Ting,ZHANG Jie,et al.Study on the Regulatory Mechanism of CB1R/BDNF in Pain Sensitization after Paradoxical Sleep Deprivation[J].Western Journal of Traditional Chinese Medicine,2025,38(06):5-12.[doi:10.12174/j.issn.2096-9600.2025.06.02]
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CB1R/BDNF调控异相睡眠剥夺诱发术后疼痛敏化的机制研究()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
38
期数:
2025年06期
页码:
5-12
栏目:
基础研究
出版日期:
2025-06-15

文章信息/Info

Title:
Study on the Regulatory Mechanism of CB1R/BDNF in Pain Sensitization after Paradoxical Sleep Deprivation
作者:
李文娟1, 周婷2, 张杰1, 薛建军1
1.甘肃省中医院，甘肃 兰州 730050
2.荆门市人民医院，湖北 荆门 448000
Author(s):
LI Wenjuan1, ZHOU Ting2, ZHANG Jie1, XUE Jianjun1
关键词:
异相睡眠剥夺; 术后疼痛; 延髓头端腹内侧部; 大麻素1型受体; 脑源性神经营养因子
Keywords:
paradoxical sleep deprivation; postoperative pain; rostral ventromedial medulla; cannabinoid type-l receptor; brain-derived neurotrophic factor
分类号:
R749.7
DOI:
10.12174/j.issn.2096-9600.2025.06.02
文献标志码:
A
摘要:
目的评估大鼠延髓头端腹内侧部（rostral ventromedial medulla，RVM）内大麻素1型受体（cannabinoid type-l receptor，CB1R）是否参与了术前异相睡眠剥夺（paradoxical sleep deprivation，PSD）诱导的切口疼痛敏化的调节，以及脑源性神经营养因子（brain-derived neurotrophic factor，BDNF）和CB1R在调节该疼痛中的相互作用。 方法将96只雄性SPF级SD大鼠采用随机数字表法分为空白组（C组）、切口组（I组）、PSD组（P组）、PSD-切口组（P-I组）、PSD-切口+CB1R拮抗剂组（P-I+SR组）、PSD-切口+CB1R激动剂组（P-I+WIN组），每组16只，建立睡眠剥夺足底切口模型，P-I+SR组和P-I+WIN组大鼠于足底切开后第1天，向大鼠RVM内注射CB1R抑制剂或CB1R激动剂。使用免疫荧光染色、免疫印迹法（Western Blotting，WB）、qPCR等分子生物学方法检测RVM中BDNF和CB1R的蛋白和mRNA表达水平。 结果与I组相比，P-I组大鼠疼痛敏感性和术后疼痛持续时间增加（P＜0.05）。与P-I组相比，P-I+WIN组大鼠术后1、4、7天CPS降低、PWT增加（P＜0.05），P-I+SR组大鼠术后1、4、7天CPS增加、PWT降低（P＜0.05）。 结论RVM内CB1R的激活降低了术前PSD诱导的切口疼痛敏化，CB1R-BDNF的相互作用可能在下游的促进调节中发挥作用。
Abstract:
ObjectiveTo evaluate whether Cannabinoid type-l receptor (CB1R) of RVM is involved in the regulation of incision pain sensitization induced by paradoxical sleep deprivation (PSD), and the interaction of BDNF and CB1R in the regulation of pain. MethodsAll 96 male SPF-grade SD rats were divided into the blank group (C group), incision group (I group), and PSD group (P group), PSD-incision group (P-I group), PSD-incision+CB1R antagonist group (P-I+SR group), and PSD-incision+CB1R agonist group (P-I+WIN group), 16 rats in each group, sleep deprivation models with plantar incision were established, P-I+SR group, and P-I+WIN group accepted the injection of CB1R inhibitors or CB1R agonists into RVM of the rats on the first day after plantar incision. Immunofluorescence staining, WB, qPCR and other molecular biological methods were used to detect the expressions of BDNF and CB1R protein and mRNA in RVM. ResultsCompared with I group, pain sensitization and the duration of postoperative pain were prolonged (P<0.05). Compared with P-I group, the decrease of CPS and the increase of PWT were found on the first, forth and seventh day after the operation in P-I+WIN group (P<0.05), while the increase of CPS and the decrease of PWT were seen on the first, forth and seventh day after the operation in P-I+SR group (P<0.05). ConclusionActivation of CB1R in RVM reduces incision pain sensitization induced by preoperative PSD, and the interaction between CB1R and BDNF may play a role in promoting downstream modulation.

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备注/Memo

备注/Memo:
李文娟（1984—），女，副主任护师。研究方向：围术期护理与术后疼痛。昆山市级科技专项项目（KJJKJZXSF2020020）。 甘肃省自然科学基金（21JR7RA586）。
更新日期/Last Update: 2025-06-15