[1]赵立涵,曾玮,周丽芳.白芍内酯苷对糖尿病大鼠肾脏损伤的作用机制研究[J].西部中医药,2025,38(12):11-16.[doi:10.12174/j.issn.2096-9600.2025.12.03]
　ZHAO Lihan,ZENG Wei,ZHOU Lifang.Study of the Mechanism of Action of Albiflorin in Alleviating Kidney Injury of Diabetic Rats[J].Western Journal of Traditional Chinese Medicine,2025,38(12):11-16.[doi:10.12174/j.issn.2096-9600.2025.12.03]
点击复制

白芍内酯苷对糖尿病大鼠肾脏损伤的作用机制研究()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
38
期数:
2025年12期
页码:
11-16
栏目:
基础研究
出版日期:
2025-12-15

文章信息/Info

Title:
Study of the Mechanism of Action of Albiflorin in Alleviating Kidney Injury of Diabetic Rats
作者:
赵立涵, 曾玮, 周丽芳
首都医科大学附属北京潞河医院,北京 101100
Author(s):
ZHAO Lihan, ZENG Wei, ZHOU Lifang
Beijing Luhe Hospital, Capital Medical University, Beijing 101100, China
关键词:
糖尿病肾病; 白芍内酯苷; 鞘氨醇激酶1-1磷酸鞘氨醇; 核因子B
Keywords:
diabetic nephropathy; albiflorin; SphK1/S1P; NF-B
分类号:
R255.4
DOI:
10.12174/j.issn.2096-9600.2025.12.03
文献标志码:
A
摘要:
目的探讨白芍内酯苷缓解糖尿病大鼠肾脏损伤的作用机制。 方法将10只雄性db/m小鼠（野生型对照）设为正常对照组，40只雄性C57BL/KsJ db/db小鼠（糖尿病模型）分为模型对照组，二甲双胍组，白芍内酯苷低、高剂量组，每组10只。除正常对照组和模型对照组每日灌胃生理盐水外，其余各组分别给予相应药物，持续干预12周。实验结束后，测定各组小鼠血糖、血清胰岛素、尿酸、肌酐、甘油三酯和总胆固醇水平，以及血清与肾脏组织中炎性细胞因子水平。采用蛋白印迹法检测肾组织中鞘氨醇激酶1（sphingosine kinase 1，SphK1）/1-磷酸鞘氨醇（sphingosine-1 1-phosphate，S1P）/核因子κB（nuclear factor kappa-light-chain-enhancer of activated B cells，NF-κB）信号通路的蛋白表达水平。 结果与正常对照组相比，模型对照组小鼠血糖、血脂、血清胰岛素及肾脏炎性因子水平均显著升高，肾组织中SphK1/S1P/NF-κB通路蛋白表达激活（P＜0.01）。与模型对照组相比，二甲双胍组和白芍内酯苷低、高剂量组均能显著改善上述指标，其中白芍内酯苷高剂量组效果最优（P＜0.01）。 结论白芍内酯苷对糖尿病大鼠肾脏损伤具有保护作用，其机制可能与调控SphK1/S1P/NF-κB信号通路有关。
Abstract:
ObjectiveTo explore the mechanism of action of albiflorin in alleviating kidney injury of diabetic rats. MethodsTen male db/m mice (wild-type control) were established as normal control group, forty male C57BL/KsJ db/db mice (diabetic models) were divided into model control group, metformin group, low and high doses groups of albiflorin with ten in each. Normal control group and model control group were drenched with physiological saline every day, other groups were administered with the corresponding medicines respectively, intervention for 12 weeks. By the end of the experiment, to detect the levels of blood glucose, serum insulin, and uric acid, serum creatinine, triglyceride and total cholesterol, inflammatory cytokines in serum and renal tissue in different groups. Western blotting was used to measure the expressions of SphK1/S1P/NF-κB signaling pathway protein in renal tissue. ResultsCompared with the normal control group, the levels of blood glucose, blood lipids, serum insulin, renal inflammatory factors in the model control group were significantly increased, and the protein expression of the SphK1/S1P/NF-κB pathway in renal tissues was activated (P<0.01). Compared with the model control group, the metformin group and the low and high dose groups of albiflorin could improve the above indicators, among which the high dose group of albiflorin showed the optimal effect (P<0.01). ConclusionAlbiflorin has protective effects on kidney damage in diabetic mice, and its mechanism might be related to the regulation of SphK1/S1P/NF-κB signaling.

相似文献/References:

[1]林晓芬,黄燕璇.益肾汤联合厄贝沙坦及胰激肽原酶治疗早期糖尿病肾病的疗效观察[J].西部中医药,2013,26(07):68.
　LIN Xiaofen,HUANG Yanxuan.Clinical Observation on YiShenTang Combined with Irbesartan and Pancreatic Kininogenase in the Treatment for Early Diabetic Nephropathy[J].Western Journal of Traditional Chinese Medicine,2013,26(12):68.
[2]王学琦,李丰华,张荣华,等.温肾汤治疗早期糖尿病肾病80例[J].西部中医药,2013,26(09):62.
　WANG Xueqi,LI Fenghua,ZHANG Ronghua,et al.WenShenTang for 80 Cases of Early Diabetic Nephropathy[J].Western Journal of Traditional Chinese Medicine,2013,26(12):62.
[3]任芳,徐厚谦△.黄芪注射液治疗糖尿病肾病有效性及安全性的系统评价[J].西部中医药,2014,27(01):77.
　REN Fang,XU Houqian.Systematic Review of the Effectiveness and Safety of HuangQi Injection in Treating Diabetic Nephropathy[J].Western Journal of Traditional Chinese Medicine,2014,27(12):77.
[4]丁夏楠,许筠△,苏建平,等.益肾逐瘀方治疗糖尿病肾病蛋白尿37例[J].西部中医药,2014,27(04):96.
　DING Xianan,XU Jun,SU Jianping,et al.Treatment for Proteinuria of 37 Patients with Diabetic Nephropathy by Kidney-invigorating Stagnation-dispelling Prescription[J].Western Journal of Traditional Chinese Medicine,2014,27(12):96.
[5]肖远莉.循证护理的优效性及影响因素分析———来自终末期糖尿病肾病患者血液透析的证据[J].西部中医药,2014,27(08):127.
[6]刘涛.缬沙坦联合复方丹参滴丸对早期2型糖尿病肾病患者尿微量白蛋白的影响[J].西部中医药,2015,28(05):106.
[7]连琯,史晓伟.当归补血汤合金水宝治疗早期糖尿病肾病16例临床观察[J].西部中医药,2013,26(06):68.
　LIAN Guan,SHI Xiaowei.Clinical Observation on DangGui BuXueTang and JinShuiBao in Treating 16 Cases of Early Diabetic Nephropathy[J].Western Journal of Traditional Chinese Medicine,2013,26(12):68.
[8]任芳,徐厚谦△.黄芪注射液治疗糖尿病肾病随机对照试验报告质量评价[J].西部中医药,2013,26(12):1.
　REN Fang,XU Houqian.Quality Evaluation of the Report for Randomized Controlled Trials of HuangQi Injection in Treating Diabetic Nephropathy[J].Western Journal of Traditional Chinese Medicine,2013,26(12):1.
[9]朱苗蕊,指导：金智生.糖脂平治疗早期糖尿病肾病60例疗效观察*[J].西部中医药,2013,26(03):74.
　ZHU Miaorui,Director: JIN Zhisheng.Clinical Observation on TangZhiPing in Treating 60 Cases of Early Diabetic Nephropathy[J].Western Journal of Traditional Chinese Medicine,2013,26(12):74.
[10]马天寿.血塞通联合西药治疗糖尿病肾病80例[J].西部中医药,2013,26(08):96.
　MA Tianshou.XueSaiTong Injection and Western Medicine in Treating 80 Cases of Diabetic Nephropathy[J].Western Journal of Traditional Chinese Medicine,2013,26(12):96.

备注/Memo

备注/Memo:
国家自然科学基金面上项目（8187150833）。赵立涵（1980—），男，副主任医师。研究方向：重症医学及全科医学。Email：13716132707@163.com。
更新日期/Last Update: 2025-12-15