藏药秀才中4种黄酮类化学成分的HPLC同时测定方法研究-《西部中医药》

文章信息/Info

Title:: Simultaneous Determination of Four Flavonoids in Tibetan Medicine "Xiucai" by HPLC

Author(s):: YANG Lijing¹, MA Mingfang¹, MI Ma², CHEN Jing²; 1.Qinghai Provincial Institute for Food Inspection and Testing, Xining 810000, China
2.Tibet University of Tibetan Medicine, Lhasa 850000, China

Keywords:: the Tibetan medicine; Chinese Juniper; Fragrant Cedar; HPLC; chemical components; content determination

摘要:: 目的建立同时测定藏药秀才中4种黄酮类化学成分含量的高效液相色谱法，为该药材的质量标准建立提供依据。方法采用高效液相色谱法，色谱柱Ultimate C₁₈（250 mm×4.6 mm，5 mL）；流动相为乙腈-4%醋酸水溶液，梯度洗脱；检测波长260 nm；流速0.8 ?L·min^-1；柱温30 ℃。结果4种指标成分分离度良好，hypolaetin-O-β-xylopyranoside、山柰酚、柏黄酮、穗花杉双黄酮的平均回收率分别为100.95%、99.19%、99.92%、101.10%，RSD分别为1.406%、1.641%、1.099%、1.72%，含量测定结果为每100 g分别含0.2729、0.2501、0.2049、0.08971 g。结论山柰酚、穗花杉双黄酮可能为藏药秀才作为五味甘露药浴治疗风湿性关节炎的活性成分。该方法快捷、简便、准确，能多成分综合评价该药材。

Abstract:: ObjectiveTo develop a high-performance liquid chromatography (HPLC) method for the simultaneous quantification of four flavonoids in Tibetan medicine Xiucai, thereby providing a scientific foundation for the establishment of its quality control standards. MethodsHPLC analysis was performed on an Ultimate C₁₈ column (250 mm × 4.6 mm, 5 mL) with gradient elution using acetonitrile–4% aqueous acetic acid as the mobile phase. The detection wavelength was set at 260 nm, the flow rate was 0.8 mL·min??, and the column temperature was maintained at 30 ℃. ResultsThe four target analytes showed good chromatographic separation. The average recoveries of hypolaetin-O-β-xylopyranoside, kaempferol, hinokiflavone, and amentoflavone were 100.95%, 99.19%, 99.92%, and 101.10%, with RSD values of 1.406%, 1.641%, 1.099%, and 1.72%, respectively. The content determination results showed that the contents of the four compounds per 100 g of the crude drug were 0.2729 g, 0.2501 g, 0.2049 g, and 0.08971 g, respectively. ConclusionKaempferol and amentoflavone may be the active components of the Tibetan medicine Xiucai in the treatment of rheumatoid arthritis as a constituent of the Wuwei Ganlu (Five-Flavor Nectar) medicinal bath. The developed method is rapid, simple, and accurate, and enables comprehensive multi-component evaluation of this medicinal material.