[1]杨丽萍,童英,马克坚,等.天麻蛋白与巴利森苷C联合应用对氧糖剥夺诱导HT22细胞损伤的协同保护作用[J].西部中医药,2026,39(09):5-9.[doi:10.12174/j.issn.2096-9600.2026.09.02]
　YANG Liping,TONG Ying,MA Kejian,et al.Synergistic Neuroprotective Effects of Gastrodin Protein and Parishin C Co-Administration on HT22 Cell Injury Induced by Oxygen-Glucose Deprivation[J].Western Journal of Traditional Chinese Medicine,2026,39(09):5-9.[doi:10.12174/j.issn.2096-9600.2026.09.02]
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天麻蛋白与巴利森苷C联合应用对氧糖剥夺诱导HT22细胞损伤的协同保护作用()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
39
期数:
2026年09期
页码:
5-9
栏目:
基础研究
出版日期:
2026-09-07

文章信息/Info

Title:
Synergistic Neuroprotective Effects of Gastrodin Protein and Parishin C Co-Administration on HT22 Cell Injury Induced by Oxygen-Glucose Deprivation
作者:
杨丽萍1, 童英1, 马克坚2, 宋娜丽2, 陈普1, 段小花1
1.云南中医药大学民族医药学院/云南省傣医药与彝医药重点实验室，云南 昆明 650500
2.云南省中医中药研究院，云南 昆明 650223
Author(s):
YANG Liping1, TONG Ying1, MA Kejian2, SONG Nali2, CHEN Pu1, DUAN Xiaohua1
1.College of Ethnic Medicine, Yunnan University of Chinese Medicine/Yunnan Key Laboratory of Dai and Yi Medicines, Kunming 650500, China
2.Yunnan Institute of Traditional Chinese Medicine and Materia Medica, Kunming 650223, China
关键词:
天麻蛋白; 巴利森苷C; HT22细胞; 缺糖缺氧/复糖复氧
Keywords:
gastrodin protein; parishin C; HT22 cell; oxygen-glucose deprivation/reperfusion
分类号:
R285.5
DOI:
10.12174/j.issn.2096-9600.2026.09.02
文献标志码:
A
摘要:
目的探究天麻蛋白和巴利森苷C（parishin C，PC）联合应用对缺糖缺氧/复糖复氧（oxygen-glucose deprivation/reperfusion，OGD/R）诱导的HT22细胞损伤的保护作用，筛选两者合用的最佳配比并明确其相互作用类型。 方法将固定浓度（15.6 μg·mL-1）的天麻蛋白分别与不同浓度（2.5、5、10、20 μmol·L-1）PC联合处理HT22细胞24 h，随后进行OGD/R处理；采用MTT法检测细胞存活率，筛选出有效且药物浓度相对较低的组合用于后续实验。检测细胞LDH漏出率；检测超氧化物歧化酶（superoxide dismutase，SOD）和谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-PX）活性以及丙二醛（malondialdehyde，MDA）含量与活性氧（reactive oxygen species，ROS）水平；流式细胞术检测细胞凋亡率，初步分析联合用药的作用效果，并采用周-特氏联合指数法和等辐射分析法明确两者的相互作用。 结果天麻蛋白与PC联合处理的细胞存活率高于单独用药组，其中有效且浓度相对较低的最佳配比为天麻蛋白15.6 μg·mL-1联合PC 10 μmol·L-1。该联合用药可降低LDH漏出率，提高SOD和GSH-PX活性，降低MDA含量及ROS水平，降低细胞凋亡率，且各项作用效果均优于单独用药组，而且天麻蛋白与PC的联合作用类型为协同作用。 结论天麻蛋白与PC联合应用对OGD/R诱导的HT22细胞损伤具有协同保护作用，其效果优于单独用药。
Abstract:
ObjectiveTo investigate the protective effects of combined application of gastrodin protein and parishin C (PC) on HT22 cell injury induced by oxygen-glucose deprivation/reperfusion (OGD/R), to screen for the optimal ratio of the two agents in combination, and to clarify the type of their interaction. MethodsHT22 cells were treated with a fixed concentration (15.6 μg·mL-1) of gastrodin protein combined with different concentrations (2.5, 5, 10, 20 μg·mL-1) of parishin C (PC) for 24 h, followed by OGD/R treatment. Cell viability was assessed by MTT assay to screen for effective combinations with relatively lower drug concentrations for subsequent experiments. To measure the lactate dehydrogenase (LDH) leakage rate. To determine the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), as well as the levels of malondialdehyde (MDA) and reactive oxygen species (ROS). Flow cytometry was employed to detect the apoptosis rate. The combined effects were preliminarily analyzed, and the type of interaction between the two agents was further clarified using the Chou-Talalay combination index method and isobolographic analysis. ResultsThe combination of gastrodin protein and PC resulted in higher cell viability compared with either agent alone. Among the effective combinations, the optimal ratio with relatively lower concentrations was identified as gastrodin protein 15.6 μg·mL-1 combined with PC 10 μg·mL-1. This combined treatment significantly decreased LDH leakage, elevated SOD and GSH-PX activities, reduced MDA and ROS levels, and lowered the apoptosis rate, all of which were superior to those observed in the monotherapy groups. Furthermore, the interaction between gastrodin protein and PC was determined to be synergistic. ConclusionThe combined application of gastrodin protein and PC exerts synergistic protective effects against OGD/R-induced HT22 cell injury, with superior efficacy to that of either agent alone.

备注/Memo

备注/Memo:
国家自然科学基金（81960733）；云南省应用基础研究面上项目（2019FB120）。杨丽萍（1985—），女，博士学位，讲师。研究方向：中药药理研究。
更新日期/Last Update: 2026-09-15