[1]李霞,朱涛,张鹿鸣,等.麦粒灸对骨髓抑制小鼠血清G-CSF及骨髓组织JAK2/STAT3蛋白磷酸化水平的影响[J].西部中医药,2026,39(09):10-13.[doi:10.12174/j.issn.2096-9600.2026.09.03]
　LI Xia,ZHU TAO,ZHANG Luming,et al.Effects of Grain-sized Moxibustion on Serum G-CSF Levels and JAK2/STAT3 Protein Phosphorylation in Bone Marrow of Mice with Myelosuppression[J].Western Journal of Traditional Chinese Medicine,2026,39(09):10-13.[doi:10.12174/j.issn.2096-9600.2026.09.03]
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麦粒灸对骨髓抑制小鼠血清G-CSF及骨髓组织JAK2/STAT3蛋白磷酸化水平的影响()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
39
期数:
2026年09期
页码:
10-13
栏目:
基础研究
出版日期:
2026-09-07

文章信息/Info

Title:
Effects of Grain-sized Moxibustion on Serum G-CSF Levels and JAK2/STAT3 Protein Phosphorylation in Bone Marrow of Mice with Myelosuppression
作者:
李霞, 朱涛, 张鹿鸣, 蔡云飞, 程艳婷
山西中医药大学，山西 晋中 030600
Author(s):
LI Xia, ZHU TAO, ZHANG Luming, CAI Yunfei, CHENG Yanting
Shanxi University of Traditional Chinese Medicine, Jinzhong 030600, China
关键词:
麦粒灸; 骨髓抑制; 细胞集落刺激因子; 磷酸化Janus酪氨酸激酶2; 磷酸化信号转导与转录激活因子3
Keywords:
grain-sized moxibustion; myelosuppression; granulocyte colony-stimulating factor; P-JAK2; P-STAT3
分类号:
R273
DOI:
10.12174/j.issn.2096-9600.2026.09.03
文献标志码:
A
摘要:
目的探讨麦粒灸对骨髓抑制小鼠血清细胞集落刺激因子（granulocyte colony-stimulating factor，G-CSF）及骨髓组织JAK2/STAT3蛋白磷酸化水平的影响。 方法将45只雄性ICR小鼠随机分为空白组、模型组、麦粒灸组，每组15只。采用腹腔注射环磷酰胺制备骨髓抑制模型。麦粒灸组在“大椎”“三阴交”“足三里”穴给予麦粒灸治疗，每日1次，共7天。在光学显微镜下用血球计数器检测白细胞数和骨髓有核细胞数，酶联免疫吸附试验检测血清粒细胞集落刺激因子（granulocyte colony-stimulating factor，G-CSF）水平，免疫印迹法（Western blot，WB）检测骨髓组织JAK2、P-JAK2、STAT3、P-STAT3蛋白表达水平。 结果3组小鼠造模前白细胞数比较差异无统计学意义（P＞0.05）；造模3天后，模型组和麦粒灸组小鼠白细胞数均低于空白组（P＜0.05）；模型组小鼠造模5天后白细胞数达到最低，造模7天后有所增加，且与空白组比较差异有统计学意义（P＜0.05）；麦粒灸组小鼠白细胞数造模5天后有所增加，其增长时间早于模型组，且与模型组比较差异有统计学意义（P＜0.05）。与空白组比较，模型组小鼠骨髓单核细胞数、血清G-CSF含量、骨髓组织P-JAK2/JAK2和P-STAT3/STAT3水平均降低（P＜0.05）。与模型组比较，麦粒灸组小鼠骨髓单核细胞数、血清G-CSF含量、骨髓组织P-JAK2/JAK2和P-STAT3/STAT3水平均升高（P＜0.05）。 结论麦粒灸可减缓骨髓抑制小鼠外周血细胞损伤和骨髓有核细胞损伤，其缓解骨髓抑制的机制可能与上调血清G-CSF含量、提高骨髓组织P-JAK2/JAK2，P-STAT3/STAT3水平，刺激骨髓组织JAK2/STAT3蛋白的磷酸化有关。
Abstract:
ObjectiveTo investigate the effects of grain-sized moxibustion on serum granulocyte colony-stimulating factor (G-CSF) and JAK2/STAT3 phosphorylation levels in bone marrow of myelosuppressed mice. MethodsForty-five male ICR mice were randomly divided into a blank group, a model group, and a grain-sized moxibustion group, with 15 mice in each group. The myelosuppression model was established by intraperitoneal injection of cyclophosphamide. Mice in the grain-sized moxibustion group received grain-sized moxibustion treatment at the "Dazhui" (GV14), "Sanyinjiao" (SP6), and "Zusanli" (ST36) acupoints once daily for seven consecutive days. White blood cell (WBC) count and bone marrow nucleated cell counts were measured using a hemocytometer under a light microscope. Serum levels of granulocyte colony-stimulating factor (G-CSF) were determined by enzyme-linked immunosorbent assay (ELISA). The protein expression levels of JAK2, p-JAK2, STAT3, and p-STAT3 in bone marrow tissue were detected by Western blot (WB) analysis. ResultsBefore modeling, there was no statistically significant difference in WBC count among the three groups (P>0.05). At three days after modeling, WBC count in both the model group and the grain-sized moxibustion group was significantly lower than that in the blank group (P<0.05). In the model group, WBC count reached their lowest level at five days after modeling and then increased at seven days after modeling, with a statistically significant difference compared with the blank group (P<0.05). In the grain-sized moxibustion group, WBC count began to increase at five days after modeling, which was earlier than that in the model group, and the difference was statistically significant compared with the model group (P<0.05). Compared with the blank group, the model group showed significant decreases in bone marrow mononuclear cell count, serum G-CSF level, and the ratios of P-JAK2/JAK2 and p-STAT3/STAT3 in bone marrow tissue (P<0.05). Compared with the model group, the grain-sized moxibustion group exhibited significant increases in bone marrow mononuclear cell count, serum G-CSF level, and the ratios of P-JAK2/JAK2 and P-STAT3/STAT3 in bone marrow tissue (P<0.05). Conclusiongrain-sized moxibustion alleviated peripheral blood cell injury and bone marrow nucleated cell injury in myelosup-pressed mice. The underlying mechanism of its ameliorative effect on myelosuppression may be associated with upregulating serum G-CSF levels, elevating the ratios of P-JAK2/JAK2 and P-STAT3/STAT3 in bone marrow tissue, and stimulating the phosphorylation of JAK2/STAT3 proteins in the bone marrow.

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备注/Memo

备注/Memo:
山西省自然科学基金（201901D111336）。李霞（1994—），女，硕士学位。研究方向：腧穴处方规范化的基础研究。
更新日期/Last Update: 2026-09-15