糖耐康对TGF-β1诱导的HK-2细胞VEGF mRNA表达的影响*-《西部中医药》

文章信息/Info

Title:: Effects of TangNaiKang on VEGF mRNA Expressions of Human Renal Tubular Epithelial Cells HK-2 Induced by TGF-β1

作者:: 朱春晖1，康开彪2，杨丽霞2，李晓东2，刘铜华3△，吴丽丽3，孙文3; 1 甘肃省人民医院，甘肃兰州 730000； 2 甘肃省中医药研究院； 3 北京中医药大学

Author(s):: ZHU Chunhui1, KANG Kaibiao2, YANG Lixia2, LI Xiaodong2, LIU Tonghua3△, WU Lili3, SUN Wen3; 1 Gansu Provincial Hospital, Lanzhou 730000, China; 2 Gansu Provincial Academy of Chinese Medicine; 3 Beijing University of Chinese Medicine

Keywords:: TangNaiKang; transforming growth factor (TGF-β1); human renal tubular epithelial cells (HK-2); vascular endothelial growth factor (VEGF) mRNA

摘要:: 目的：通过观察糖耐康药物血清干预转化生长因子-β1(TGF-β1)诱导的人肾小管上皮细胞(HK-2)、血管内皮生长因子(VEGF)mRNA的表达，进一步探讨其在防治肾间质纤维化方面的作用。方法：将HK-2细胞用含10%胎牛血清的DMEM/F12(1∶1)培养基培养；实验分为6组：空白对照组、单纯TGF-β1诱导组(TGF-β110 ng/mL)、空白血清对照组(TGF-β110 ng/mL+10%空白血清)、干预1组(TGF-β110 ng/mL +5%糖耐康药物血清)、干预2组(TGF-β110 ng/mL+10%糖耐康药物血清)、干预3组(TGF-β110 ng/mL +20%糖耐康药物血清)。药物干预24小时后，荧光定量PCR检测VEGF mRNA的表达。结果：HK-2细胞经TGF-β1诱导后，VEGF mRNA的表达显著上升，与空白对照组相比有统计学意义(P＜0.05)，经糖耐康药物血清干预后，VEGF mRNA的表达逐步下降，与单纯TGF-β1诱导组相比有统计学意义(P＜0.05)。结论：糖耐康能够抑制TGF-β1诱导的人肾小管上皮细胞VEGF mRNA的表达，在一定程度上具有抑制肾小管上皮细胞纤维化的作用。

Abstract:: Objective: To explore the mechanism of TangNaiKang in preventing and treating renal interstitial fibrosis through observing the intervention of TangNaiKang on vascular endothelial growth factor(VEGF) mRNA expressions of human renal tubular epithelial cells(HK-2) induced by transforming growth factor (TGF-β1). Method: HK-2 cells were cultured in the media of DMEM/F12 (1:1) containing 10% fetal bovine serum; the cells were divided into six groups: blank control group, TGF-β1 group(TGF-β110 ng/mL), blank serum control group(TGF-β1 10 ng/mL+10% blank serum), intervention group one(TGF-β110 ng/mL+5% medicated serum of TangNaiKang), intervention group two(TGF-β1 10 ng/mL+10% medicated serum of TangNaiKang) and intervention group three (TGF-β110 ng/mL+20% medicated serum of TangNaiKang). After intervened for 24 hours,VEGF mRNA expressions were detected with fluorescence quantitative PCR. Result: VEGF mRNA expressions were raised significantly when HK-2 cells were induced by TGF-β1, it had statistical meaning compared with blank control group(P＜0.05), VEGF mRNA expressions were decreased gradually after intervened with medicated serum of TangNaiKang, it had statistical meaning compared with TGF-β1 group(P＜0.05). Conclusion: TangNaiKang could inhibit VEGF mRNA expressions of HK-2 induced by TGF-β1 and the fibrosis of renal tubular epithelial cells to a certain extent.