[1]韩世龙,杨玉华,牛承成.HPLC法测定苁黄补肾片中松果菊苷的含量*[J].西部中医药,2014,27(03):40-42.
　HAN Shilong,YANG Yuhua,NIU Chengcheng.Determination of Echinacoside in CongHuang BuShen Tablets by HPLC[J].Western Journal of Traditional Chinese Medicine,2014,27(03):40-42.
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HPLC法测定苁黄补肾片中松果菊苷的含量*
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
27
期数:
2014年03期
页码:
40-42
栏目:
出版日期:
2014-03-15

文章信息/Info

Title:
Determination of Echinacoside in CongHuang BuShen Tablets by HPLC
文章编号:
1004-6852(2014)03-0040-03
作者:
韩世龙，杨玉华，牛承成
兰州佛慈制药股份有限公司，甘肃 兰州 730064
Author(s):
HAN Shilong, YANG Yuhua, NIU Chengcheng
Lanzhou Foci Pharmaceutical Co., LTD, Lanzhou 730064, China
关键词:
苁黄补肾片; 松果菊苷; HPLC; 含量测定
Keywords:
CongHuang BuShen tablets;  echinacoside;  HPLC;  content determination
分类号:
R284.1
文献标志码:
A
摘要:
目的：建立苁黄补肾片中松果菊苷的含量测定方法。方法:采用高效液相色谱法，色谱柱：TSK-GEL DDS-C18，(4.6 mm×250 mm，5 μm)，流动相：甲醇-0.1%甲酸溶液(25∶75)，流速：1.0 mL/min，柱温：40℃，进样量：10 μL，检测波长：330 nm。结果：松果菊苷在0.075 2～7.52 μg范围内呈良好的线性关系，r=0.999 978，平均回收率为96.95％，RSD=1.59％。结论：该方法测定苁黄补肾片中松果菊苷的含量简便可行、灵敏、准确、重现性好，可用于苁黄补肾片的质量控制。
Abstract:
Objective: To establish the determination method of the contents of echinacoside in CongHuang BuShen tablets by HPLC. Methods: HPLC was adopted, chromatographic column: TSK-GEL DDS-C18 (4.6 mm×250 mm, 5 μm), mobile phase: methanol-0.1% methanoic acid (25:75), flow rate: 1.0mL/min, column temperature:40℃, sample size: 10 μL, detection wavelength: 330 nm. Results: Echinacoside showed better linear relationship in the range between 0.0752 and 7.52 μg, r=0.999 978, average recovery rate was 96.95%, RSD=1.59%. Conclusion: The method is simple, feasible, sensitive, accurate and reproducible, it could be used to determinate the contents of echinacoside from CongHuang BuShen tablets and quality control of CongHuang BuShen tablets.

备注/Memo

备注/Memo:
收稿日期：2013-07-11*基金项目：2010年甘肃省第二批科技计划(科技重大专项)（编号1002FKDA041）作者简介：韩世龙(1974—)，男，工程师。研究方向：新药制剂研发。
更新日期/Last Update: 2014-03-15