[1]梁曦,薛建军△.甘草喷雾剂对大鼠全麻气管插管后呼吸道黏膜及细胞因子的影响[J].西部中医药,2017,30(02):16-19.
　LIANG Xi,XUE Jianjun.Effects of Licorice Spray on Respiratory Tract Mucosa and Cytokines of Rats after General Anesthesia Tracheal Intubation[J].Western Journal of Traditional Chinese Medicine,2017,30(02):16-19.
点击复制

甘草喷雾剂对大鼠全麻气管插管后呼吸道黏膜及细胞因子的影响()
分享到：

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
30
期数:
2017年02期
页码:
16-19
栏目:
出版日期:
2017-02-15

文章信息/Info

Title:
Effects of Licorice Spray on Respiratory Tract Mucosa and Cytokines of Rats after General Anesthesia Tracheal Intubation
文章编号:
1004-6852(2017)02-0016-04
作者:
梁曦，薛建军△
甘肃省中医院麻醉科，甘肃 兰州 730050
Author(s):
LIANG Xi, XUE Jianjun△
LIANG Xi, XUE Jianjun△
关键词:
甘草喷雾剂; 气管插管; 呼吸道黏膜; 大鼠; 动物实验
Keywords:
licorice spray;  tracheal intubation;  mucous membrane of respiratory tract;  rats;  animal experiment
分类号:
R285.5
文献标志码:
A
摘要:
目的：探讨甘草喷雾剂对实验大鼠全麻气管插管后呼吸道黏膜组织病理学变化及血清肿瘤坏死因子α(TNF-α)、白介素1(IL-1)及白介素10(IL-10)含量的影响。方法：选择50只SPF级Wistar大鼠，雌雄各半，按体质量随机分为生理盐水组，利多卡因组，甘草喷雾高、中、低剂量组，每组10只。采用气管插管法制作动物模型。持续插管60分钟后，拔出气管导管，分别摘取各组动物咽喉部黏膜及其下组织用10%福尔马林溶液固定，切片，进行病理组织学检查。各组动物股动脉取血，离心抽取血清，检测TNF-α、IL-1及IL-10的含量。结果：大鼠血清TNF-α、IL-1、IL-10含量及炎细胞浸润程度评分甘草喷雾高、中剂量组与生理盐水组、甘草喷雾低剂量组比较，差异均有统计学意义(P＜0.05)；甘草喷雾高、中剂量组与利多卡因组比较，差异均无统计学意义(P＞0.05)；甘草喷雾高剂量组与甘草喷雾中剂量组比较，差异均无统计学意义(P＞0.05)。甘草各剂量组黏膜上皮表面炎性渗出均较生理盐水组减轻，固有膜内炎细胞浸润均较生理盐水组减轻。结论：甘草喷雾剂对气管插管后呼吸道黏膜具有保护作用。
Abstract:
Objective: To study the effects of licorice spray on pathological changes of respiratory mucosa and content of TNF-α, IL-1 and IL-10 in rats which were given general anesthesia tracheal intubation. Methods: Fifty SPF Wistar rats in half genders were selected and randomly divided into normal saline group, lidocaine group and low, middle and high dose groups of licorice spray according to the weight, each group 10 rats. Tracheal intubation was adopted to establish animal models. Tracheal tube was pulled out after 60 minutes of continuous intubation, and then the mucous membranes of the throat and tissues from each animal were obtained, fixed in 10% formalin solution, sectioned and histopathology examinations were performed. The blood was drawn from the femoral artery, centrifugal extraction of serum was made, the contents of TNF-α, IL-1 and IL-10 were tested. Results: The score of content of TNF-α, IL-1 and IL-10 and the degree of infiltration of inflammatory cells were compared between high and middle dose groups of licorice spray with normal saline group and low dose group of licorice spray, the differences were statistically significant (P＜ 0.05). The differences between high and middle dose groups licorice spray and lidocaine group were not significant (P＞0.05). There was no significant differences between high and middle dose groups of licorice spray(P＞0.05). Inflammatory exudate in the surface of epithelium mucosa and intrinsic membrane inflammatory cells infiltration in all dosage licorice spray groups were less than that in saline group. Conclusion: Licorice spray has protective effects on mucous membrane of respiratory tract after tracheal intubation.

相似文献/References:

[1]杨鹏,杨世祥,董斌.中西医结合治疗重症有机磷中毒的临床观察[J].西部中医药,2015,28(09):99.
[2]张雪霞,李岩,李文娟.40例全麻气管插管咽喉部不适患者辨证护理效果分析[J].西部中医药,2015,28(12):134.
[3]白芮,李文娟.双黄连超声雾化吸入对全麻插管患者术后呼吸道症状的干预与护理[J].西部中医药,2013,26(11):120.
　BAI Rui,LI Wenjuan.Treatment and Care for Postoperative Respiratory Symptoms of the Patients with Anesthesia and Incubation by Ultrasonic Atomizing Inhalation of ShuangHuangLian[J].Western Journal of Traditional Chinese Medicine,2013,26(02):120.
[4]杨开银,薛建军,梁曦,等.甘草喷雾剂预防全麻气管插管应激反应及术后并发症临床观察[J].西部中医药,2021,34(09):103.[doi:10.12174/j.issn.2096-9600.2021.09.28]
　YANG Kaiyin,XUE Jianjun,LIANG Xi,et al.Clinical Observation on Licorice Spray in the Prevention of Stress Response of Endotracheal Intubation Under General Anesthesia and Postoperative Complications[J].Western Journal of Traditional Chinese Medicine,2021,34(02):103.[doi:10.12174/j.issn.2096-9600.2021.09.28]

备注/Memo

备注/Memo:
收稿日期：2016-08-18*基金项目：甘肃省中医药管理局项目(编号GZK-2012-67)。作者简介：梁曦(1982—)，女，硕士学位，主治医师。研究方向：内科疾病的中西医结合诊治及临床麻醉。△通讯作者：薛建军(1971—)，男，硕士学位，主任医师。研究方向：临床麻醉。
更新日期/Last Update: 2017-02-15