[1]康开彪,颜春鲁△,姚贞宇,等.敦煌医方大补肾汤对镉染毒模型大鼠肝组织细胞因子和氧化应激的影响[J].西部中医药,2018,31(03):12-15.
　KANG Kaibiao,YAN Chunlu,YAO Zhenyu,et al.The Influence of Dunhuang Medical Prescription DaBuShen Decoction on Oxidative Stress and Cytokines in Liver Tissue of Cadmium Poisoning Rat Model[J].Western Journal of Traditional Chinese Medicine,2018,31(03):12-15.
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敦煌医方大补肾汤对镉染毒模型大鼠肝组织细胞因子和氧化应激的影响()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
31
期数:
2018年03期
页码:
12-15
栏目:
出版日期:
2018-03-15

文章信息/Info

Title:
The Influence of Dunhuang Medical Prescription DaBuShen Decoction on Oxidative Stress and Cytokines in Liver Tissue of Cadmium Poisoning Rat Model
文章编号:
1004-6852(2018)03-0012-04
作者:
康开彪1，颜春鲁2△，姚贞宇3，辛阳2
1 甘肃省中医药研究院，甘肃 兰州730050； 2 甘肃中医药大学； 3 兰州市第一人民医院
Author(s):
KANG Kaibiao1, YAN Chunlu2△, YAO Zhenyu3, XIN Yang2
1 Gansu Provincial Academy of Chinese Medicine, Lanzhou 730050, China;2 Gansu University of Chinese Medicine; 3 The First People′s Hospital of Lanzhou City
关键词:
镉染毒; 氧化损伤; 免疫功能; 大补肾汤
Keywords:
cadmium poisoning;  oxidative damage;  immunologic function;  DaBuShen decoction
分类号:
R711
摘要:
目的：探讨大补肾汤对镉染毒模型大鼠肝组织细胞因子和氧化应激的影响。方法：将SPF级Wistar大鼠60只随机分为正常对照组、模型组、乙酰半胱氨酸组及大补肾汤高、中、低剂量组。末次给药24小时后处死大鼠，计算胸、脾指数。采用比色分析法测定大鼠肝组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量，酶联免疫吸附试验(ELISA)测定大鼠肝组织白细胞介素2(IL-2)和转化生长因子β1(TGF-β1)的含量。结果：与正常对照组比较，模型组大鼠体质量明显减轻(P＜0.05)；大补肾汤中、高剂量组大鼠体质量明显高于模型组(P＜0.05)。模型组胸、脾指数低于正常对照组，差异有统计学意义(P＜0.05)；大补肾汤各剂量组大鼠脾脏指数高于模型组，且高剂量组胸腺指数高于模型组，差异有统计学意义(P＜0.05)。与正常对照组比较，模型组MDA含量升高、SOD 活性降低(P＜0.05)；大补肾汤各剂量组MDA含量低于模型组，SOD活性高于模型组(P＜0.05)。模型组IL-2含量低于正常对照组，TGF-β1含量高于正常对照组(P＜0.05)；大补肾汤各剂量组IL-2含量高于模型组，TGF-β1含量低于模型组(P＜0.05)。结论：大补肾汤对镉所致大鼠的氧化损伤和免疫损伤具有保护作用。
Abstract:
Objective: To discuss the influence of DaBuShen decoction on oxidative stress and cytokines in liver tissue of the rat model suffering cadmium poisoning. Methods: Sixty Wistar rats were randomized into normal control group, the model group, acetylcysteine group, high, moderate and low doses groups of DaBuShen decoction. The rats were sacrificed in 24 hours after the last medication, spleen and thymus indexes were calculated, the activity of SOD and the contents of MDA in liver tissueof the rats were measured by using colorimetric analysis, the contents of IL-2 and TGF-β1 in liver tissuewere detected by ELISA method. Results: Compared with normal control group, body weight of the rats in the model group reduced obviously(P＜0.05); high and moderate dose groups of DaBuShen decoction were notably higher than the model group in body weight of the rats remarkably (P＜0.05). The model group was lower than normal control group in spleen and thymus indexes obviously, and the difference showed statistical meaning(P＜0.05); different doses groups of DaBuShen decoction were higher than the model group in the spleen index of the rats, and high dose group of DaBuShen decoction was higher than the model group in thymus index obviously, and the difference showed statistical meaning (P＜0.05). Compared with normal control group, the contents of MDA lifted apparently while the activity of SOD lowered notably in the model group (P＜0.05); different doses groups of DaBuShen decoction were lower than the model group in the contents of MDA notably, higher than the model group in the activity of SOD notably(P＜0.05). The model group was lower than normal control group in the contents of IL-2 notably, higher than normal control group in the contents of TGF-β1 (P＜0.05); different doses groups of DaBuShen decoction were higher than the model group in the contents of IL-2 notably, lower than the model group in the contents of TGF-β1(P＜0.05). Conclusion: DaBuShen decoction shows protective effects to oxidative damage and immunologic injury of the rat models suffering cadmium poisoning.

相似文献/References:

[1]颜春鲁,李盛华△,刘永琦,等.敦煌医方小补肾汤对镉染毒大鼠骨和肾功能的影响[J].西部中医药,2017,30(01):11.
　YAN Chunlu,LI Shenghua,et al.The Influence of Dunhuang Prescription Xiao BuShen Tang on Bone and Renal Functions of the Rats Exposed to Cadmium[J].Western Journal of Traditional Chinese Medicine,2017,30(03):11.

备注/Memo

备注/Memo:
收稿日期：2016-12-27 *基金项目：甘肃省自然科学基金项目(编号1508RJZA046)；甘肃中医药大学教学研究与教学改革项目(编号ZH-201606)；敦煌医学与转化省部共建教育部重点实验室开放基金项目(编号DHYX1213-004)。 作者简介：康开彪(1979—)，男，硕士学位，副主任医师，第六批全国老中医药专家学术经验继承人。研究方向：中医内科及医学编辑。 △通讯作者：颜春鲁（1979—），男，在读博士研究生，副教授。研究方向：骨伤疾病的中医药防治。
更新日期/Last Update: 2018-03-15