北败酱提取物的体外抗氧化抗炎活性研究-《西部中医药》

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Title:: A Study on Antioxidant and Anti-inflammatory Activities of Extracts from Beibaijiang in Vitro

文章编号:: 1004-6852(2020)08-0021-03

作者:: 孙晓春1,2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2，雷莉妍1,2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2，黄文静1,2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2，宋忠兴1,2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2，唐志书1,2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2△; 1 陕西中医药大学陕西省中药资源产业化协同创新中心，陕西咸阳 712083;2 陕西省中药基础与新药研究重点实验室

Author(s):: SUN Xiaochun1，2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2, LEI Liyan1，2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2, HUANG Wenjing1，2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2, SONG Zhongxing1，2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2, TANG Zhishu1，2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2△; 1 Shaanxi Collaborative Innovation Center of Chinese Medicinal Resource Industrialization of Shaanxi University of Chinese Medicine, Xianyang 712083, China; 2 Shaanxi Provincial Key Lab of the Study for Traditional Chinese Medicine and New Drugs

关键词:: 北败酱; 水提物; 醇提物; 抗氧化; 抗炎

Keywords:: Beibaijiang; water extracts; alcohol extracts; antioxidant; anti-inflammatory

分类号:: R285

DOI:: 10.12174/j.issn.1004-6852.2020.08.06

文献标志码:: A

摘要:: 目的：评价北败酱水提物和醇提物的体外抗氧化活性和抗炎活性。方法：采用3种抗氧化体系检测提取物清除羟基、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基和超氧阴离子的能力，通过检测脂多糖(lipopolysaccharide，LPS)诱导的一氧化氮(nitric oxide，NO)生成量评价其抗炎活性。结果：浓度为10 mg/mL时，北败酱水提物和醇提物对羟基自由基（hydroxyl radical,·OH）的清除率分别为84.18%和32.93%，对DPPH·的清除率分别为45.33%和38.18%，浓度为1 mg/mL时，对O2-·的清除率分别为55.09%和44.09%。对LPS诱导的NO生成均具有抑制作用，并且呈量效关系。北败酱水提物和醇提物浓度为5 mg/mL时，NO的生成量由48.47 μmol/L分别降低到20.99 μmol/L和12.35 μmol/L。结论：北败酱水提物和醇提物均具有一定的抗氧化能力和抗炎活性，并且水提物的活性高于醇提物的活性。

Abstract:: Objective: To assess antioxidant and anti-inflammatory activities of water extracts and alcohol extracts from Beibaijiang (herba patrinine) in vitro. Methods: Three kinds of antioxidant systems were used to detect the ability of the extracts removing hydroxyl, DPPH free radical and superoxide anion, its anti-inflammatory activity was evaluated through detecting LPS-induced NO yield. Results: When the concentrations were 10 mg/mL, ·OH clearance rates of water extracts and alcohol extracts from Beibaijiang were 84.18% and 32.93% respectively, DPPH clearance rate were 45.33% and 38.18% respectively, when the concentrations were 1 mg/mL, O2-· clearance rates were 55.09% and 44.09% respectively. It owned the inhibitory effects on NO generation induced by LPS, in the dose-effect relationship. When the concentrations of water extracts and alcohol extracts from Beibaijiang were 5 mg/mL, NO yield lowered from 48.47 μmol/L to 20.99 μmol/L and 12.35 μmol/L respectively. Conclusion: Water extracts and alcohol extracts from Beibaijiang possess certain antioxidant capacity and anti-inflammatory activities, the activities of water extracts are higher than these of alcohol extracts.

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

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