[1]王竹,孙万森,刘俊田.积雪草苷对足细胞α-actinin-4 、synaptopodin蛋白及ERK/JNK通路的影响[J].西部中医药,2021,34(07):23-29.[doi:10.12174/j.issn.2096-9600.2021.07.06]
　WANG Zhu,SUN Wansen,LIU Juntian.Influence of Asiaticoside on Podocyte α- actinin-4, synaptopodin Protein and ERK/JNK Pathway[J].Western Journal of Traditional Chinese Medicine,2021,34(07):23-29.[doi:10.12174/j.issn.2096-9600.2021.07.06]
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积雪草苷对足细胞α-actinin-4 、synaptopodin蛋白及ERK/JNK通路的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
34
期数:
2021年07期
页码:
23-29
栏目:
出版日期:
2021-07-15

文章信息/Info

Title:
Influence of Asiaticoside on Podocyte α- actinin-4, synaptopodin Protein and ERK/JNK Pathway
作者:
王竹1, 孙万森1, 刘俊田2
1.西安交通大学医学院第二附属医院中医科，陕西 西安 710004
2.西安交通大学医学院药理系
Author(s):
WANG Zhu1, SUN Wansen1, LIU Juntian2
1.Department of Traditional Chinese Medicine, The Second Affiliated Hospital, Xi’an Jiaotong University Health Science Center, Xi’an 710004, China
2.Department of Pharmacology, Xi’an Jiaotong University Health Science Center
关键词:
足细胞-actinin-4蛋白; synaptopodin蛋白; ERK/JNK通路; 积雪草苷; 阿霉素
Keywords:
podocyte - actinin-4 protein; synaptopodin protein; ERK/JNK pathway; sasiaticoside; adriamycin
分类号:
R285.5
DOI:
10.12174/j.issn.2096-9600.2021.07.06
摘要:
目的探讨积雪草苷对阿霉素诱导的足细胞中α-actinin-4、synaptopodin蛋白及ERK/JNK通路的影响。 方法1）MTT法检测积雪草苷对足细胞及阿霉素诱导的足细胞活力的影响。2）选用1 μmol/L阿霉素制造足细胞损伤模型，蛋白印迹法（Western blotting）检测足细胞α-actinin-4、synaptopodin蛋白表达。3）免疫荧光检测足细胞骨架及Western blotting检测ERK/JNK信号通路的磷酸化水平。 结果1）低浓度积雪草苷（10～80 μg/mL）对足细胞活力无明显影响，而高浓度积雪草苷（＞80 μg/mL）可降低足细胞活力；2）阿霉素体外可损伤足细胞，降低足细胞活力；积雪草苷浓度依赖性地减轻阿霉素对足细胞的损伤，增加足细胞活力；阿霉素体外可增加足细胞骨架蛋白α-actinin-4的表达，减少足细胞骨架蛋白synaptopodin的表达，而积雪草苷可下调阿霉素诱导的足细胞α-actinin-4蛋白表达，上调阿霉素抑制的足细胞synaptopodin的表达；3）阿霉素（1 μmol/L）能升高ERK、JNK的磷酸化水平，与空白对照组比较差异有统计学意义（P＜0.05）；积雪草苷预处理细胞后，能够有效遏制阿霉素诱导的ERK活化，与模型对照组比较差异有统计学意义（P＜0.05），但却不能抑制阿霉素诱导的JNK信号通路中JNK的活化。 结论积雪草苷预处理细胞后可能通过遏制阿霉素诱导的ERK信号通路的活化，减少阿霉素对足细胞骨架的损伤作用，从而改变了α-actinin-4、synaptopodin蛋白表达。
Abstract:
ObjectiveTo explore the influence of asiaticoside on podocyte α- actinin-4, synaptopodin protein and ERK/JNK pathways induced by adriamycin. Methods1) MTT method was used to detect the effects of asiaticoside on podocyte and podocyte viability induced by adriamycin. 2)Podocyte injury model was established by choosing adriamycin at the dose of 1 μmol/L, Western blotting to detect the expressions of α- actinin-4 and synaptopodin protein in podocyte. 3) Immunofluorescence to measure podocyte skeleton, Western blotting to measure the phosphorylation level of ERK/JNK pathways. Results1)Low concentrations of asiaticoside (10 to 80 μg/mL) showed no influence on podocyte viability, while high concentrations of asiaticoside (>80 μg/mL) could lower podocyte viability; 2) Adriamycin in vitro could cause the damage to podocyte and lower podocyte viability; asiaticoside could relieve adriamycin-induced damage to podocyte and increase podocyte viability in dose-dependent manner; adriamycin in vitro could increase the expressions of podocyte cytoskeleton protein α- actinin-4 and lower the expressions of podocyte cytoskeleton protein synaptopodin, while asiaticoside could down regulate the expressions of podocyte α-actinin-4 protein induced by adriamycin, up regulate the expressions of podocyte synaptopodin inhibited by adriamycin; 3) Adriamycin (1μmol/L) could lift phosphorylation of ERK and JNK, the difference had statistical meaning when it was compared with blank control group (P<0.05); after preconditioning cells, asiaticoside could effectively inhibit ERK activation induced by adriamycin, the difference showed statistical meaning compared with model control group (P<0.05), while it can not inhibit JNK activation induced by adriamycin. ConclusionAsiaticoside pretreatment could change the expressions of α-actinin-4 and synaptopodin protein possibly through inhibiting the activation of ERK signaling pathway induced by adriamycin and reducing adriamycin-induced damage to podocyte cytoskeleton.

备注/Memo

备注/Memo:
王竹（1979—），女，博士学位，副主任医师。研究方向：肾脏疾病的中西医结合治疗。陕西省重点研发计划（2017SF-331）。
更新日期/Last Update: 2021-07-15