[1]彭琼琳,黄敏,孙敏.当归芍药散预处理对缺血/再灌注损伤小鼠离体子宫生理特性的影响[J].西部中医药,2021,34(10):34-38.[doi:10.12174/j.issn.2096-9600.2021.10.08]
　PENG Qionglin,HUANG Min,SUN Min.Influence of Danggui Shaoyao Powder Preconditioning on Physiological Characteristics of Isolated Uterus in Mice with Ischemia/Reperfusion Injury[J].Western Journal of Traditional Chinese Medicine,2021,34(10):34-38.[doi:10.12174/j.issn.2096-9600.2021.10.08]
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当归芍药散预处理对缺血/再灌注损伤小鼠离体子宫生理特性的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
34
期数:
2021年10期
页码:
34-38
栏目:
出版日期:
2021-10-15

文章信息/Info

Title:
Influence of Danggui Shaoyao Powder Preconditioning on Physiological Characteristics of Isolated Uterus in Mice with Ischemia/Reperfusion Injury
作者:
彭琼琳1, 黄敏1, 孙敏2
1.鄂钢医院，湖北 鄂州 436000
2.十堰市太和医院/湖北医药学院附属医院
Author(s):
PENG Qionglin1, HUANG Min1, SUN Min2
1.Egang Hospital, Ezhou 436000, China
2.Affiliated Taihe Hospital of Hubei University of Medicine
关键词:
缺血/再灌注损伤; 当归芍药散; 离体子宫平滑肌条; 线粒体呼吸链酶; Ca; 生理特性; 动物实验
Keywords:
ischemia/reperfusion injury; powder; isolated uterine smooth muscle strip; mitochondrial respiratory chain enzyme; Ca; physiological function; zoopery
分类号:
R711
DOI:
10.12174/j.issn.2096-9600.2021.10.08
摘要:
目的观察当归芍药散预处理对缺血/再灌注损伤模型小鼠离体子宫生理特性的影响并分析其可能机制。 方法将60只雌性小鼠随机分为空白对照组，模型组，中药高、中、低剂量组，每组12只。各组给予对应药物处理，预处理后建立子宫缺血/再灌注损伤动物模型。成模后2 h取小鼠右侧离体子宫平滑肌条检测子宫肌条钙离子（Ca2+）浓度、超氧化物歧化酶（superoxide dismutase，SOD）、乳酸脱氢酶（lactate dehydrogenase，LDH）、丙二醛（malondialdehyde，MDA）及线粒体呼吸链酶复合体Ⅰ、Ⅱ、Ⅲ、Ⅳ水平，记录5组小鼠离体子宫平滑肌条生理特性的改变情况。 结果与模型组比较，中药中、高剂量组收缩力、频率、子宫活动力、LDH、MDA、线粒体呼吸链酶复合体Ⅰ、Ⅱ、Ⅲ、Ⅳ及Ca2+浓度均明显降低，并恢复到空白对照组水平，SOD则明显高于模型组和中药低剂量组（P＜0.05），且这种效应存在剂量依赖性，中药高、中剂量组组间比较，差异有统计学意义（P＜0.05）。 结论当归芍药散可能通过抑制线粒体呼吸链酶这一细胞能量代谢途径，阻碍Ca2+内流而降低离体子宫平滑肌对缩宫素的敏感性，这会使子宫活动力降低，在一定程度上促进了缺血/再灌注子宫生理功能的恢复。
Abstract:
ObjectiveTo observe the impacts of Danggui Shaoyao powder preconditioning on physiological characteristics of isolated uterus in mice with ischemia/reperfusion injury, and analyze its possible mechanism. MethodsSixty female mice were randomized into blank control group, the model group, high, moderate and low dosages groups of herbs, 12 mice in each group. Different groups were given the corresponding medicine, after preconditioning to establish the models of uterine ischemia/reperfusion injury. Smooth muscle strips of isolated right mice uterus were taken out two hours after modeling to measure the concentrations of Ca2+ in myometrium, the levels of SOD, LDH, MDA, mitochondrial respiratory chain enzyme complex Ⅰ,Ⅱ, Ⅲ and Ⅳ, to record the changes of physiological characteristics of isolated uterine smooth muscle strip of the mice in five groups. ResultsCompared with the model group, contractility, frequency, uterine activity, LDH, MDA, the concentrations of mitochondrial respiratory chain enzyme complex Ⅰ,Ⅱ, Ⅲ and Ⅳ, and Ca2+ of the moderate and high doses groups of herbs lowered notably, and these restored to the levels of blank control group, the levels of SOD were higher than these of the model group and low dose group of herbs (P<0.05), and the effect was dose-dependent, the difference was statistically significant between high and moderate doses groups of herbs (P<0.05). ConclusionDanggui Shaoyao powder could reduce the sensitivity of isolated uterine smooth muscle to oxytocin which might be through inhibiting mitochondrial respiratory chain enzyme, which is the energy metabolism pathway of cells, and blocking Ca2+ influx, it would reduce the activity of uterus and promote the recovery of physiological function of ischemia/reperfusion uterus to a certain extent.

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备注/Memo

备注/Memo:
彭琼琳（1982—），女，主治医师。研究方向：妇产科相关疾病的诊治。国家自然科学基金青年科学基金（81902498）。
更新日期/Last Update: 2021-10-15