[1]何敏,岳鹏莹,白宝平.苦参碱通过Pink1/Parkin途径抑制线粒体途径促进肝癌细胞凋亡的机制?[J].西部中医药,2021,34(11):50-55.[doi:10.12174/j.issn.2096-9600.2021.11.11]
　HE Min,YUE Pengying,BAI Baoping.Mechanism of Matrine Inhibiting Mitochondrial Pathway and Promoting Apoptosis of Hepatoma Cells through Pink1/Parkin Pathway[J].Western Journal of Traditional Chinese Medicine,2021,34(11):50-55.[doi:10.12174/j.issn.2096-9600.2021.11.11]
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苦参碱通过Pink1/Parkin途径抑制线粒体途径促进肝癌细胞凋亡的机制?

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
34
期数:
2021年11期
页码:
50-55
栏目:
出版日期:
2021-11-15

文章信息/Info

Title:
Mechanism of Matrine Inhibiting Mitochondrial Pathway and Promoting Apoptosis of Hepatoma Cells through Pink1/Parkin Pathway
作者:
何敏, 岳鹏莹, 白宝平
延安大学西安创新学院，陕西 西安 710100
Author(s):
HE Min, YUE Pengying, BAI Baoping
Xi'an Innovation College of Yan'an University, Xi'an 710100, China
关键词:
肝癌细胞; Pink1/Parkin信号通路; 增殖; 凋亡; 侵袭
Keywords:
hepatoma cells; Pink1/Parkin pathway; proliferation; apoptosis; invasion
分类号:
R735.7
DOI:
10.12174/j.issn.2096-9600.2021.11.11
文献标志码:
A
摘要:
目的探讨苦参碱通过Pink1/Parkin途径抑制线粒体途径促进肝癌细胞凋亡的作用机制。 方法培养人源性肝癌细胞系HepG2，按照干预条件分成对照组、Pink1/Parkin信号通路抑制剂3-MA抑制剂组及0.5、1.0 g/L苦参碱处理组，采用反转录-聚合酶链反应（reverse transcription-polymerase chain reaction，RT-PCR）、免疫蛋白印迹（Western Blot）和免疫荧光检测Pink1和Parkin以及凋亡信号蛋白Bcl-2、Bad、Bax、Caspase-3、Caspase-6和Caspase-9等表达，应用CCK-8试剂盒检测细胞增殖能力、Transwell试剂盒检测细胞侵袭能力、AV-PI试剂盒检测细胞凋亡率。 结果RT-PCR、Western Blot结果显示：0.5和1.0 g/L苦参碱处理组Pink1 含量低于对照组，差异具有统计学意义（P＜0.05）；1.0 g/L处理组Pink1 mRNA含量低于0.5 g/L处理组，差异具有统计学意义（P＜0.05）；0.5和1.0 g/L苦参碱处理组Pink1 mRNA含量低于抑制剂组，差异具有统计学意义（P＜0.05）；对照组Pink1 mRNA含量与抑制剂组比较差异无统计学意义（P＞0.05）。Parkin和Bcl-2的表达趋势与Pink1相同，而凋亡相关因子Bad、Bax、Caspase-3、Caspase-6和Caspase-9等的表达与Pink1相反。CCK-8、Transwell检测结果显示：0.5和1.0 g/L苦参碱处理组细胞增殖能力与细胞侵袭能力均低于对照组，差异具有统计学意义（P＜0.05）；对照组细胞增殖能力、侵袭能力与抑制剂组比较差异无统计学意义（P＞0.05）。AV-PI结果显示：0.5和1.0 g/L苦参碱处理组细胞侵袭能力低于对照组，差异具有统计学意义（P＜0.05）；对照组细胞侵袭能力与抑制剂组比较差异无统计学意义（P＞0.05）。 结论苦参碱可以抑制肝癌细胞的增殖和侵袭能力，促进肝癌细胞凋亡，并且具有剂量依赖性。
Abstract:
ObjectiveTo explore the mechanism of matrine inhibiting mitochondrial pathway and promoting apoptosis of hepatoma cells through Pink1/Parkin pathway. MethodsAfter culturing human hepatoma cell line HepG2, the cells were divided into the control group, Pink1/Parkin signaling pathway inhibitor 3-MA inhibitor group and matrine processing groups of 0.5 g/L and 1.0 g/L according to the intervention conditions, RT-PCR, Western Blot and immunofluorescence were adopted to detect the contents of Pink1 and Parkin, the expressions of apoptosis signal proteins Bcl-2, Bad, Bax, Caspase-3, Caspase-6, Caspase-9 and others, CCK-8 test kit was applied to measure cell proliferation ability, Transwell test kit to determine cell invasion ability,AV-PI test kit to measure cell apoptosis rate. ResultsThe results of RT-PCR, Western Blot displayed that the contents of Pink1 in the matrine processing groups of 0.5 and 1.0 g/L were lower than these of the control group, and the difference had statistical meaning (P<0.05); the contents of Pink1 mRNA in 1.0 g/L matrine processing group were lower than these of 0.5 g/L matrine processing group, and the difference had statistical meaning (P<0.05); the contents of Pink1 mRNA in matrine processing groups of 0.5 and 1.0 g/L were lower than these in inhibitor group, and the difference was statistically significant (P<0.05); the difference had no statistical meaning when the contents of Pink1 mRNA in the control group were compared with these in inhibitor group (P>0.05), expression trend of Parkin and Bcl-2 was the same as that of Pink1, while the apoptosis related factors Bad, Bax, Caspase-3, Caspase-6 and Caspase-9 were opposite to Pink1. The results of CCK-8 and Transwell results showed that the cell proliferation ability and cell apoptosis rate of matrineprocessing groups of 0.5 g/L and 1.0 g/L were lower than these of the control group, and the difference had statistical meaning (P<0.05). The difference had no statistical meaning when cell proliferation ability and invasion ability of the control group were compared with these of the inhibitor group (P>0.05). AV-PI results showed that the invasion ability of matrine processing groups of 0.5 g/L and 1.0 g/L was lower than that of the control group, and the difference showed statistical meaning (P<0.05); there was no statistical difference in the comparison of invasion ability between the control group and the inhibitor group (P>0.05). ConclusionMatrine could inhibit the proliferation and invasion ability of hepatoma cells, promote the apoptosis of hepatoma cells in dose-dependent manner.

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备注/Memo

备注/Memo:
何敏（1984—），女，硕士学位，讲师。研究方向：公共卫生。陕西省教育厅2021年度专项科学研究计划（21JK0998）。
更新日期/Last Update: 2022-08-09