[1]徐世红,柳博,谢兴文,等.蒲公英水提液不同萃取部位对人骨肉瘤MG-63细胞的影响[J].西部中医药,2022,35(05):21-24.[doi:10.12174/j.issn.2096-9600.2022.05.05]
　XU Shihong,LIU Bo,XIE Xingwen,et al.Effects of Different Parts of Dandelion Water Extract on Human Osteosarcoma MG-63 Cells[J].Western Journal of Traditional Chinese Medicine,2022,35(05):21-24.[doi:10.12174/j.issn.2096-9600.2022.05.05]
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蒲公英水提液不同萃取部位对人骨肉瘤MG-63细胞的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
35
期数:
2022年05期
页码:
21-24
栏目:
出版日期:
2022-05-15

文章信息/Info

Title:
Effects of Different Parts of Dandelion Water Extract on Human Osteosarcoma MG-63 Cells
作者:
徐世红1,2, 柳博3, 谢兴文4, 李鼎鹏1,2, 许伟2, 丁聚贤3, 黄睿3, 姜朝阳3
1.甘肃省中医药研究院，甘肃 兰州 730050
2.甘肃省中医院
3.甘肃中医药大学
4.甘肃省第二人民医院
Author(s):
XU Shihong1,2, LIU Bo3, XIE Xingwen4, LI Dingpeng1,2, XU Wei2, DING Juxian3, HUANG Rui3, JIANG Chaoyang3
1.Gansu Provincial Academy of Chinese Medicine, Lanzhou 730050, China
2.Gansu Provincial Hospital of Traditional Chinese Medicine
3.Gansu University of Chinese Medicine
4.Second Provincial People's Hospital of Gansu
关键词:
骨肉瘤; MG-63传代细胞; 萃取物; 蒲公英
Keywords:
osteosarcoma; MG-63 passage cells; extract; dandelion
分类号:
R965.1
DOI:
10.12174/j.issn.2096-9600.2022.05.05
文献标志码:
A
摘要:
目的探讨蒲公英水提液不同萃取部位对人骨肉瘤MG-63细胞的细胞形态及增殖的影响。 方法将蒲公英加水煎煮得蒲公英水提液，然后用石油醚、乙酸乙酯和正丁醇依次以1∶1比例萃取蒲公英水提物，得到不同溶剂萃取部位；人骨肉瘤MG-63细胞传代贴壁培养后应用蒲公英水提液不同萃取部位培养；采用四甲基偶氮唑盐(methyl thiazolyl tetrazdium，MTT)法检测蒲公英水提液各萃取部位在不同时间内对人骨肉瘤细胞MG-63的增殖抑制作用。 结果蒲公英水提液各萃取部位在体外对MG-63细胞增殖具有抑制作用，呈现出剂量与时间的效应关系。 结论蒲公英水提液各萃取部位能抑制人骨肉瘤MG-63细胞，在体外有一定抗肿瘤活性，其中以乙酸乙酯萃取部位最有效。
Abstract:
ObjectiveTo explore the effects of different parts of dandelion water extract on the cellular morphology and proliferation of human osteosarcoma(OS) MG-63 cells. MethodsWater extract of dandelion was obtained by decocting the whole dandelion with water; different solvent extracts were got by extracting dandelion water extract with petroleum ether, ethyl acetate and n-butanol in the ratio of 1:1, different extracts of dandelion were applied to culture human osteosarcoma MG-63 cells after passage and adherent culture; MTT method was adopted to detect the inhibitory effects of dandelion water extract on the proliferation of human osteosarcoma MG-63 cells at different times. ResultsDandelion water extract could inhibit the proliferation of MG-63 cells in vitro, presenting in the dose-time concentration-effect relationship. ConclusionDandelion water extract could inhibit human osteosarcoma MG-63 cells, it has certain anti-tumor activity in vitro, and dandelion ethyl acetate extract is particularly the most effective.

相似文献/References:

[1]谢兴文,徐世红,李晶,等.骨肉瘤相关细胞因子水平的诊断与测定[J].西部中医药,2015,28(10):147.
[2]乔莉,谢兴文,徐世红,等.骨肉瘤中Runx3基因的表达及其对肿瘤细胞生物学行为的影响[J].西部中医药,2019,32(08):158.
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[3]潘海达,秦刚.基于异病同治及数据挖掘探讨八珍汤治疗股骨头坏死及骨肉瘤的机制[J].西部中医药,2025,38(01):88.[doi:10.12174/j.issn.2096-9600.2025.01.17]
　PAN Haida,QIN Gang.Potential Mechanism of Treating Osteonecrosis of the Femoral Head and Osteosarcoma with Bazhen Tang Based on Data Mining and Same Treatment for Different Diseases[J].Western Journal of Traditional Chinese Medicine,2025,38(05):88.[doi:10.12174/j.issn.2096-9600.2025.01.17]

备注/Memo

备注/Memo:
徐世红（1983—），男，硕士学位，主治医师。研究方向：骨与软组织肿瘤的中医药防治。甘肃省青年科技基金（18JR3RA068）。
更新日期/Last Update: 2022-06-24