[1]蔺海生,尤从新,赵宁,等.消肿止痛合剂对大鼠骨骼肌损伤后MyoD蛋白及mRNA表达的影响[J].西部中医药,2026,39(06):5-9.[doi:10.12174/j.issn.2096-9600.2026.06.02]
　LIN Haisheng,YOU Congxin,ZHAO Ning,et al.Effects of Xiaozhong Zhitong Mixture on the Expressions of MyoD Protein and mRNA in Rats after Skeletal Muscle Injury[J].Western Journal of Traditional Chinese Medicine,2026,39(06):5-9.[doi:10.12174/j.issn.2096-9600.2026.06.02]
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消肿止痛合剂对大鼠骨骼肌损伤后MyoD蛋白及mRNA表达的影响()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
39
期数:
2026年06期
页码:
5-9
栏目:
基础研究
出版日期:
2026-06-08

文章信息/Info

Title:
Effects of Xiaozhong Zhitong Mixture on the Expressions of MyoD Protein and mRNA in Rats after Skeletal Muscle Injury
作者:
蔺海生1, 尤从新1, 赵宁1, 李兴勇2
1.甘肃省中医院/甘肃中医药大学第一附属医院，甘肃 兰州 730050
2.甘肃省人民医院，甘肃 兰州 730030
Author(s):
LIN Haisheng1, YOU Congxin1, ZHAO Ning1, LI Xingyong2
1.Gansu Provincial Hospital of Traditional Chinese Medicine/ The First Affiliated Hospital of Gansu University of Chinese Medicine, Lanzhou 730050, China
2.Gansu Provincial Hospital, Lanzhou 730030, China
关键词:
骨骼肌损伤; 消肿止痛合剂; MyoD mRNA; 修复再生
Keywords:
skeletal muscle injury; Mixture; MyoD mRNA; repair and regeneration
分类号:
R285.5
DOI:
10.12174/j.issn.2096-9600.2026.06.02
文献标志码:
A
摘要:
目的基于骨骼肌损伤后模型大鼠骨骼肌组织生肌决定因子（myogenic determination factor，MyoD）蛋白及mRNA的表达，探讨消肿止痛合剂对损伤后的骨骼肌修复再生的影响。 方法将80只实验大鼠通过随机数字表法分为空白组、对照组、模型组及治疗组，每组20只，每组大鼠再随机分为1天、7天、14天及21天组，每组5只。空白组予生理盐水灌胃；对照组予布洛芬溶液灌胃；模型组予生理盐水灌胃；治疗组按10 mL·kg-1剂量灌胃消肿止痛合剂。干预结束后，利用RT-PCR法检测MyoD mRNA表达水平，蛋白免疫印迹法检测MyoD蛋白表达水平。 结果与空白组比较，第1天模型组骨骼肌中MyoD蛋白及mRNA表达无明显差异（P＞0.05），第7、14、21天模型组骨骼肌中MyoD蛋白及mRNA表达随着时间延长，逐渐上调（P＜0.05）；与模型组比较，第7、14天治疗组骨骼肌中MyoD蛋白及mRNA表达上调（P＜0.05）。 结论消肿止痛合剂能够通过上调MyoD蛋白及mRNA表达，促进骨骼肌损伤后的修复及再生。
Abstract:
ObjectiveTo investigate the effect of Xiaozhong Zhitong Mixture on the repair and regeneration of injured skeletal muscle based on the expression of myogenic determination factor (MyoD) protein and mRNA in the skeletal muscle tissue of rat models after skeletal muscle injury. MethodsA total of 80 experimental rats were randomly divided into a blank group, a control group, a model group, and a treatment group using a random number table method, with 20 rats in each group. Rats in each group were further randomly subdivided into four subgroups according to the time points of day 1, day 7, day 14, and day 21, with 5 rats in each subgroup. The blank group received normal saline by gavage; the control group received ibuprofen solution by gavage; the model group received normal saline by gavage; and the treatment group received Xiaozhong Zhitong Mixture at a dose of 10 mL/kg by gavage. After the intervention, the expression level of MyoD mRNA was detected by RT-PCR, and the expression level of MyoD protein was detected by Western blotting. ResultsCompared with the blank group, there was no significant difference in the expression levels of MyoD protein and mRNA in the skeletal muscle of the model group on day 1 (P > 0.05). However, on days 7, 14, and 21, the expression levels of MyoD protein and mRNA in the skeletal muscle of the model group were gradually up-regulated over time (P < 0.05). Compared with the model group, the expression levels of MyoD protein and mRNA in the skeletal muscle of the treatment group were significantly up-regulated on days 7 and 14 (P < 0.05). Conclusion Xiaozhong Zhitong Mixture can promote the repair and regeneration of injured skeletal muscle by up-regulating the expression of MyoD protein and mRNA.

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备注/Memo

备注/Memo:
甘肃省自然科学基金（22JR5RA641）。蔺海生（1989—），男，硕士学位，主治医师。研究方向：中医药防治骨伤科疾病。
更新日期/Last Update: 2026-06-15