药膳“益脾饮”对脾气虚证大鼠血液流变学及血浆TXB2、6—keto

文章信息/Info

Title:: Influence of YiPiYin on Hemorheology and TXB2, 6-Keto-PGF1α of Plasma in Rats with Syndrome of Spleen-Qi Deficiency

Author(s):: DING Lian-cui, LU Xin-guo; School of Tourism and Culinary Science of Yangzhou University, Yangzhou 225127, China

Keywords:: YiPiYin; syndrome of spleen-Qi deficiency; hemorheology; TXB2; 6-keto-PGF1α; animal experiment

摘要:: 目的：探讨药膳益脾饮对脾气虚证大鼠血液流变学及血浆TXB2、6-keto-PGF1α水平的影响。方法：将48只SD大鼠随机分为6组，除空白对照组外，其余建立脾气虚模型。脾气虚模型设益脾饮高、中、低剂量干预组，四君子汤阳性对照组，模型组(以生理盐水干预)，持续给药4周。第4周末分别取血测定大鼠血液流变学及血浆TXB2、6-keto-PGF1α水平。结果：与空白对照组比较，脾气虚模型组大鼠全血高、中、低切黏度和血浆黏度、TXB2、6-keto-PGF1α均显著升高，差异有统计学意义(P＜0.01)；益脾饮高、中、低剂量组及四君子汤阳性对照组的全血黏度、血浆黏度及TXB2、6-keto-PGF1α分别与模型组比较均有所降低，差异有统计学意义(P＜0.05)，与空白对照组相比差异无统计学意义(P＜0.05)。结论：脾气虚证大鼠存在血液高黏及易形成血栓状态，药膳益脾饮干预4周对上述指标有显著改善作用。

Abstract:: Objective: To investigate the effects of YiPiYin on hemorheology, TXB2 and 6-keto-PGF1α of plasma in rats with syndrome of spleen-Qi deficiency. Method: Forty-eight SD rats were randomly divided into six groups. Except these in blank control group, the others were set to be the models with spleen-qi deficiency. The models with intervention were separated into high, moderate, low dose groups of YiPiYin, control group of SiJunZiTang and model group(intervened with physiological saline) for four weeks. Hemorheology, TXB2 and 6-keto-PGF1α of plasma in rats were detected by the end of forth week. Result: Compared with blank control group, high, moderate, low shear viscosity in blood, plasma viscosity, TXB2 and 6-keto-PGF1α in model group of spleen-Qideficiency raised obviously with remarkable difference(P＜0.01); whole blood viscosity, plasma viscosity, TXB2 and 6-keto-PGF1α in high, moderate, low dose group of YiPiYin and positive control group of SiJunZiTang were lower than in model group with statistical meaning (P＜0.05), but close to the results of blank control group without remarkable difference. Conclusion: Intervention with YiPiYin for four weeks can significantly improve blood hyperviscosity and easy formation of thrombus in rats with syndrome of spleen-Qi deficiency.