[1]徐强,宋宝丽,崔向东,等.白藜芦醇对过氧化损伤人牙周膜细胞DNA的保护作用*[J].西部中医药,2013,26(08):5-7.
 XU Qiang,SONG Baoli,CUI Xiangdong,et al.Protection of Resveratrol on DNA of Human Periodontal Ligament Cells Damaged by Peroxidation[J].Western Journal of Traditional Chinese Medicine,2013,26(08):5-7.
点击复制

白藜芦醇对过氧化损伤人牙周膜细胞DNA的保护作用*
分享到:

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
26
期数:
2013年08期
页码:
5-7
栏目:
方药·药理研究
出版日期:
2013-08-15

文章信息/Info

Title:
Protection of Resveratrol on DNA of Human Periodontal Ligament Cells Damaged by Peroxidation
文章编号:
1004-6852(2013)08-0005-03
作者:
徐强宋宝丽崔向东白玉蓉
兰州市中医医院,甘肃 兰州 730020
Author(s):
XU Qiang SONG Baoli CUI Xiangdong BAI Yurong
Lanzhou Municipal TCM Hospital, Lanzhou 730020, China
关键词:
人牙周膜细胞过氧化单细胞凝胶电泳白藜芦醇
Keywords:
human periodontal ligament cells peroxidation single cell gel electrophoresis (SCGE) resveratrol
分类号:
R780.2
文献标志码:
A
摘要:
目的:建立人牙周膜细胞过氧化损伤体外模型,通过单细胞凝胶电泳技术(SCGE)观察白藜芦醇对过氧化损伤人牙周膜细胞DNA的保护作用。方法:采用H2O2建立人牙周膜细胞过氧化损伤模型,将原代培养的人牙周膜细胞分为对照组、损伤组和保护组,采用不同浓度的H2O2及白藜芦醇进行干预,应用SCGE检测细胞DNA损伤变化。结果:随H2O2浓度的增加,人牙周膜细胞DNA的损伤程度加重,尾长、尾部DNA百分含量和尾距均增加(P<0.05);白藜芦醇使H2O2损伤的人牙周膜细胞的尾长、尾部DNA百分含量和尾距均较对照组减少(P<0.05)。结论:白藜芦醇可有效减少过氧化损伤引起的细胞DNA断裂。
Abstract:
Objective: To establish periodontal ligament cell models damaged by peroxidation in vitro and observe the protection of resveratrol on DNA of human periodontal ligament cells through the technique of single cell gel electrophoresis (SCGE). Method: Peroxidation damaged models of human periodontal ligament cells were established with hydrogen peroxide; primary cultured human periodontal ligament cells were divided into the control group, the damage group and the protection group. The cells were intervened with different concentrations of hydrogen peroxide and resveratrol, the changes of DNA damage were detected with SCGE. Result: As the concentration of hydrogen peroxide increased, the degree of DNA damage of Human periodontal ligament cells aggravated, the percentage content of DNA in the tail length, the tail and the tail distance increased (P<0.05). The group of resveratrol was less than the control group in the percentage content of DNA in the tail length, the tail and the tail distance (P<0.05). Conclusion: Resveratrol could effectively reduce DNA break induced by peroxidation.

相似文献/References:

[1]赵弼洲,田佳灵,余占海△,等.淫羊藿苷对人牙周膜细胞OCN表达水平的影响[J].西部中医药,2014,27(01):26.
 ZHAO Bizhou,TIAN Jialing,YU Zhanhai,et al.Effects of Icariin on the Expression of OCN in Human Periodontal Ligament Cells[J].Western Journal of Traditional Chinese Medicine,2014,27(08):26.

备注/Memo

备注/Memo:
收稿日期:2013-03-14 *基金项目:甘肃省自然科学基金项目(编号1208RJZA176) 作者简介:徐强(1961—),男,副主任医师。研究方向:口腔疾病的临床治疗与基础研究。
更新日期/Last Update: 2013-08-15