甘肃乌拉尔甘草有效菌株内生细菌活性物质初步研究*-《西部中医药》

文章信息/Info

Title:: Preliminary Study on Active Substances of Endophytic Fungus in Effective Strain of Gansu Ural Licorice

Author(s):: DENG Yi1, 2, DING Renwei1, REN Pengfei1; 1 Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China; 2 Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines of the College of Gansu Province

Keywords:: Gansu Ural Licorice; endophytic fungus; glycyrrhizic acid; bacteriostasis; active substance

摘要:: 目的：考察甘肃乌拉尔甘草具有抑菌作用的内生细菌的活性物质。方法：利用HPLC检测，以甘草苷、甘草酸单铵盐标准品为参照，对甘草有效菌株内生细菌JTYB-001、JTYB-053、JTZB-001发酵液抑菌活性成分进行分析。结果：三菌株代谢产物出峰时间分别为(RT=21.071分钟)、(RT=21.041)、(RT=21.067)，与甘草酸单铵盐标准品的出峰时间(RT=21.031分钟)相近，采用面积法对其定量，产量分别约为0.033、0.005，0.013 mg/mL。结论：甘肃乌拉尔甘草野生与栽培品有效菌株内生细菌JTYB-001、JTYB-053、JTZB-001三菌株分别含有与宿主相同药理活性的甘草酸类物质。

Abstract:: Objective: To investigate active substance of endophytic fungus in Gansu ural licorice. Method: Bacteriostatic active substance of endophytic fungus JTYB-001, JTYB-053 and JTZB-001 fermentation broth in effective strains of licorice were detected by HPLC and analyzed by taking glycyrrhizin and glycyrrhizic acid as the indexes. Result: The peak times of three strains metabolite were respectively (RT=21.071min), (RT=21.041) and (RT=21.067), they were closed to the peak time of mono-ammonium glycyrrhizinate standard substance (RT=21.031 minutes), their quantification and yields were respectively 0.033, 0.005 and 0.013mg/mL by area method. Conclusion: Endophytic fungus JTYB-001, JTYB-053 and JTZB-001 of effective strain in wild and cultivated Gansu ural licorice are found to contain glycyrrhizic acid identical with the host which owns pharmacological activity.