[1]景姗,王新月,杨雪,等.溃疡性结肠炎大鼠肺、肠组织中TGF-β1、SP-A表达的改变及中药的干预作用*[J].西部中医药,2014,27(03):10-15.
 JING Shan,WANG Xinyue,YANG Xue,et al.The Expressions of TGF-β1 and SP-A in the Lung and the Intestines of Rats with Ulcerative Colitis and TCM Intervention on the Changes[J].Western Journal of Traditional Chinese Medicine,2014,27(03):10-15.
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溃疡性结肠炎大鼠肺、肠组织中TGF-β1、SP-A表达的改变及中药的干预作用*
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
27
期数:
2014年03期
页码:
10-15
栏目:
方药·药理研究
出版日期:
2014-03-15

文章信息/Info

Title:
The Expressions of TGF-β1 and SP-A in the Lung and the Intestines of Rats with Ulcerative Colitis and TCM Intervention on the Changes
文章编号:
1004-6852(2014)03-0010-06
作者:
景姗1王新月2杨雪3杨舒2盛益华2闫昕2
1 南通市中医院,江苏 南通 226001; 2 北京中医药大学东直门医院; 3 河南中医学院第三附属医院
Author(s):
JING Shan1 WANG Xinyue2 YANG Xue3 YANG Shu2 SHENG Yihua2 YAN Xin2
1 Nantong Municipal TCM Hospital, Nantong 226001, China; 2 Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine; 3 The Third Affiliated Hospital to Henan University of TCM
关键词:
结肠炎溃疡性转化生长因子-β1(TGF-β1)肺表面活化蛋白-A(SP-A)肺与大肠相表里大鼠中药干预
Keywords:
ulcerative colitis TGF-β1 SP-A the lung and large intestine being interiorly-exteriorly related rat TCM intervention
分类号:
R285.5
文献标志码:
A
摘要:
目的:通过观察溃疡性结肠炎(UC)大鼠模型肺、肠组织中转化生长因子-β1(TGF-β1)、肺表面活化蛋白-A(SP-A)表达的改变及中药的干预作用,探讨UC发生肺损伤的机制,并为临床治疗提供实验依据。方法:用结肠黏膜致敏加三硝基苯磺酸-50%乙醇灌肠的方法建立UC大鼠模型,并进行中药复方(从肺论治组和从肠论治组)、中药单体(苦参碱及鱼腥草素钠)及西药对照(柳氮磺胺吡啶)灌胃干预,分别于给药0、4周取材,采用双抗体夹心ELISA法检测肺、肠组织及血清中TGF-β1的含量,采用Western blot法检测肺、肠组织中SP-A蛋白表达水平。结果:0、4周时模型组血清中TGF-β1含量明显升高;0周时,模型组肺、肠组织中抗炎性因子TGF-β1表达增多,4周时表达下降,治疗后各组TGF-β1均有上升趋势,从肺论治组肺组织中TGF-β1表达升高最为明显,而从肠论治组、苦参碱组和鱼腥草素钠组能明显升高肠组织中TGF-β1表达水平;0、4周时,肺组织中SP-A表达明显降低,治疗并不能促进其表达;0、4周时肠组织SP-A mRNA、SP-A表达明显升高,各治疗组均能明显降低其表达。结论:4周时,TGF-β1含量在血清中升高而在肺、肠组织中下降,说明UC的肺部炎症反应不是缘于局部对整体的影响,而是具有组织特异性;归经于肺的鱼腥草素钠能明显升高肠道TGF-β1表达水平,说明肺肠的特殊联系,即肺、肠组织在结构上存在共同的物质基础SP-A,且与UC发生肺损伤的机制相关。
Abstract:
Objective: To explore the mechanism of lung injury in the rats suffering from ulcerative colitis (UC) and provide experimental evidence for clinical treatment by observing the changes of TGF-β1 and SP-A expressions in the lung and the intestines of the rats with UC. Methods: Rat models with UC were established by colon mucous membrane sensitization and enema with trinitrobenzene sulfonic acid-50% ethanol, all intervened with herbal compound prescriptions (the groups of treatment based on the lung and the intestine), herbal monomers (matrine and sodium houttuyfonate) and Western medicine (salazosulfapyridine) by intragastric administration, the contents of TGF-β1 and SP-A in the lung, intestines and the serum were detected with double-antibody sandwich ELISA method in the beginning and in four weeks after medicated, the expressions of SP-A in the lung and intestines were detected with Western blot method. Results: The contents of TGF-β1 in the serum were improved significantly in the beginning and four weeks after medicated; the expressions of TGF-β1 in the lung and intestines of model group were increased in the beginning while lowered in the four weeks, and the contents of TGF-β1 were increased after treating in different groups, the rise of TGF-β1 expressions of the lung in the group of treatment based on the lung was the most obvious, the levels of TGF-β1 expressions of the intestines in the group of treatment based on the intestines, the groups of matrine and sodium houttuyfonate were improved significantly; the expressions of SP-A in lung were lowered significantly, the treatment couldn't promote its expressions; the expressions of SP-A mRNA and SP-A of the intestines were improved evidently in the beginning and in four weeks after medication, the indexes in the others were decreased obviously. Conclusion: After medicated for four weeks, the contents of TGF-β1 in the serum of model group are raised obviously but decreased in the lung and the intestines, which demonstrates that it owns tissue specificity but not caused by the influence of the local to the whole; sodium houttuyfonate of the lung meridian could notably raise the expressions of TGF-β1 in the intestines, it suggests that special relationship between the lung and the intestines, SP-A is existed in the lung and the intestines, related to the mechanism of lung injury of UC rats.

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备注/Memo

备注/Memo:
收稿日期:2013-07-29*基金项目:国家重点基础研究发展计划(973计划)项目(编号2009CB522705)作者简介:景姗(1984—),女,博士学位,主治医师。研究方向:脾胃病学。
更新日期/Last Update: 2014-03-15