[1]郭敏,姜华,肖正国.高效液相色谱法同时测定三黄中空栓中黄芩苷、盐酸小檗碱的含量*[J].西部中医药,2014,27(09):19-21.
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高效液相色谱法同时测定三黄中空栓中黄芩苷、盐酸小檗碱的含量*
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《西部中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
27
期数:
2014年09期
页码:
19-21
栏目:
方药·质量分析
出版日期:
2014-09-15

文章信息/Info

作者:
郭敏1姜华2肖正国1
1 甘肃省中医院,甘肃 兰州 730050; 2 甘肃省中医药研究院
关键词:
三黄中空栓黄芩苷盐酸小檗碱高效液相色谱
分类号:
R286
摘要:
目的:建立三黄中空栓中黄芩苷和盐酸小檗碱的含量测定方法。方法:采用高效液相色谱法,色谱柱为Waters Symmetry Shield-C18柱(4.6 mm×250 mm,5 μm),以0.1%磷酸水溶液-乙腈(50:50)为流动相,流速为1.0 mL/min,检测波长265 nm,柱温为30℃进行检测。结果:黄芩苷线性范围为5.0~101.0 μg/mL,平均加样回收率为98.80%(RSD%=1.17%);盐酸小檗碱线性范围为5.7~114.0 μg/mL,平均加样回收率为99.00%(RSD%=1.15%)。结论:该法快速、简便,结果准确、可靠,可用于三黄中空栓中黄芩苷和盐酸小檗碱含量的测定

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备注/Memo

备注/Memo:
*基金项目:甘肃省自然科学基金计划项目(编号2012-258)作者简介:郭敏(1983—),女,硕士学位,主管药师。研究方向:中(藏)药有效成分筛选及质量分析。
更新日期/Last Update: 2014-09-15