[1]吴红梅,苟于强,胡林海,等.纹党参多糖联合环磷酰胺对S180荷瘤小鼠抗肿瘤增效机制的研究[J].西部中医药,2016,29(04):17-21.
　WU Hongmei,GOU Yuqiang,HU Linhai,et al.Study on Synergetic Mechanism of Polysaccharide Extracted from WenXian DangShen Combined with Cyclophosphamide in Fighting against S180 Tumor of the Mice[J].Western Journal of Traditional Chinese Medicine,2016,29(04):17-21.
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纹党参多糖联合环磷酰胺对S180荷瘤小鼠抗肿瘤增效机制的研究()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
29
期数:
2016年04期
页码:
17-21
栏目:
出版日期:
2016-04-15

文章信息/Info

Title:
Study on Synergetic Mechanism of Polysaccharide Extracted from WenXian DangShen Combined with Cyclophosphamide in Fighting against S180 Tumor of the Mice
文章编号:
1004-6852(2016)04-0017-05
作者:
吴红梅1，苟于强2，胡林海3，胡芳弟4△，陈嘉屿5，杨春霞4，白瑞斌4
1 甘肃省中医学校，甘肃 兰州 730050； 2 兰州军区疾病预防控制中心； 3 嘉峪关市第一人民医院；4 兰州大学药学院； 5 兰州军区兰州总医院消化科
Author(s):
WU Hongmei1, GOU Yuqiang2, HU Linhai3, HU Fangdi4△, CHEN Jiayu5, YANG Chunxia4, BAI Ruibin4
1 Gansu Provincial TCM School, Lanzhou 730050, China;2 Lanzhou Military Region Center for Disease Control and Prevention；3 Jiayuguan Municipality the First People′s Hospital; 4 Pharmacy College of Lanzhou University; 5 Gastroenterology Department of Lanzhou General Hospital of Lanzhou Command
关键词:
纹党参多糖; 环磷酰胺; 抗肿瘤; 增效
Keywords:
the polysaccharide extracted from WenXian DangShen;  cyclophosphamide;  anti-tumor;  synergy
分类号:
R285.5
摘要:
目的:研究纹党参多糖对环磷酰胺抗肿瘤作用的增效效应及作用机制。方法:选用健康成年昆明种小鼠135只，随机分为9组：正常对照组(ZD组)，荷瘤对照组(HD组)，环磷酰胺组(CP组)，纹党参多糖高(WCPP-G组)、中(WCPP-Z组)、低(WCPP-D组)剂量治疗组及WCPP-G、WCPP-Z、WCPP-D联合CP组，每组15只。除ZD组小鼠外，将0.2 mL(1×1010个/L)肿瘤细胞接种于各组小鼠腋窝皮下。ZD组和HD组给予0.5 mL生理盐水灌胃，6小时后给予等体积蒸馏水，1次/d；CP组0.5 mL生理盐水灌胃，6小时后腹腔注射环磷酰胺20 mg/(kg·d)；WCPP-G、WCPP-Z、WCP-D组分别给予高［400 mg/(kg·d)］、中［200 mg/(kg·d)］、低［100 mg/(kg·d)］浓度0.5 mL党参多糖水溶液灌胃，6小时后腹腔注射0.5 mL生理盐水；WCPP-G、WCPP-Z、WCP-D联合CP组分别给予高、中、低浓度0.5 mL党参多糖水溶液灌胃，6小时后腹腔注射环磷酰胺20 mg/(kg·d)。20天后计算抑瘤率，测定免疫相关指标及血清细胞因子IL-2、IL-6、IL-1β、IL-4、INF-γ、TNF-α的含量。结果：与ZD组比较，HD组IL-2、IL-6、TNF-α、INF-γ水平下降，其中TNF-α变化不明显，差异无统计学意义(P＞0.05)，其余各项变化明显，差异有统计学意义(P＜0.05)。与HD组比较，CP组IL-2、IL-1β、IL-6、IL-4、TNF-α水平降低，其中INF-γ、IL-2水平变化不明显，差异无统计学意义(P＞0.05)，其余各项变化明显，差异有统计学意义(P＜0.05)；IL-2、IL-6、IL-1β、IL-4、TNF-α、INF-γ水平各剂量WCPP组及其联合CP组均显著增高，差异有统计学意义(P＜0.05)。与HD组及CP组比较，WCPP-G、Z组及各剂量WCPP联合CP组IL-2、IL-6水平变化明显差异有统计学意义(P＜0.05)，各剂量WCPP组及其联合CP组IL-1β和TNF-α水平均有明显变化，差异有统计学意义(P＜0.05)。与CP组比较，WCPP-D组IL-2水平变化明显，差异有统计学意义(P＜0.05)；WCPP-Z、WCPP-D组IL-4水平变化明显，差异有统计学意义(P＜0.05)。与HD组比较，各剂量WCPP组及其联合CP组IL-4和INF-γ水平变化明显，差异有统计学意义(P＜0.05)。WCPP各组的抑瘤率均低于CP组；WCPP-G、WCPP-Z、WCP-D+CP组的抑瘤率高于CP组。结论：纹党参多糖能增强环磷酰胺的抗小鼠S180肿瘤作用，这可能与其能提高荷瘤小鼠外周血IL-2水平， 降低IL-4水平逆转Th1/Th2漂移，促进免疫细胞的活化和增殖，激活免疫细胞对瘤细胞的特异性和非特异性杀伤有关。
Abstract:
Objective: To study the synergy between the polysaccharide extracted from WenXian DangShen [Codonopsis pilosula. Var . modesta (Nannf) L. T. shen] and cyclophosphamide fighting against tumor and its mechanism. Methods: All 135 mice were randomized into nine groups: normal control group(ZD group), cancer-bearing control group (HD group), cyclophosphamide group(CP group), the treatment groups of high(WCPP-G group), moderate(WCPP-Z group) and low(WCPP-D group) doses of the polysaccharide extracted from WenXian DangShen, and WCPP-G, WCPP-Z and WCPP-D combined with CP groups, 15 mice each group. Except the mice in ZD group, 0.2mL(1×1010/L) tumor cells were transplanted under the skin of the armpit of the mice in different groups. ZD group and HD groups were given with intragastric administration of 0.5mL physiological saline, and the distilled water in the same amount in six hours, once per day; CP group accepted intragastric administration of 0.5mL physiological saline, and peritoneal injection of cyclophosphamide 20 mg/(kg·d) in six hours; WCPP-G, WCPP-Z and WWCP-D groups accepted intragastric administration of polysaccharide aqueous solution in high [400 mg/(kg·d)], moderate [200 mg/(kg·d)] and low [100 mg/(kg·d)] concentrations, and peritoneal injection of 0.5mL physiological saline in six hours; WCPP-G, WCPP-Z and WWCP-D combined with CP groups received 0.5mL polysaccharide aqueous solution in high, moderate and low concentrations by lavage respectively, and peritoneal injection of cyclophosphamide 20mg/(kg·d) in six hours; tumor inhibition rate was calculated in 20 days, the contents of immune-related indexes, serum cytokines including IL-2, IL-6, IL-1β, IL-4, INF-γ and TNF-α were detected. Results: Compared with ZD group, the levels of IL-2, IL-6, INF-γ and TNF-α decreased, while the changes of TNF-α changed indistinctly in HD group (P＞0.05), and others changed evidently (P＜0.05). Compared with HD group, the levels of IL-2, IL-1β, IL-6, IL-4 and TNF-α lowered in CP group, while the levels of INF-γ and IL-2 changed insignificantly (P＞0.05), others changes obviously (P＜0.05); the levels of IL-2, IL-6, IL-1β, IL-4, TNF-α and INF-γ rose evidently in WCPP groups in different doses and WCPP combine with CP groups (P＜0.05). Compared with HD group and CP group, the levels of IL-2 and IL-6 changed significantly in WCPP-G, Z group and WCPP combined with CP groups in different doses (P＜0.05). The levels of IL-1β and TNF-α showed obvious changes in WCPP groups in different doses and WCPP combined with CP groups (P＜0.05). Compared with CP group, the levels of IL-2 changed notably in WCPP-D group (P＜0.05); the levels of IL-4 changed notably in WCPP-Z and WCPP-D groups (P＜0.05). Compared with HD group, the levels of IL-4 and INF-γ changed apparently in WCPP groups in different doses and WCPP combined with CP groups (P＜0.05). WCPP groups were lower than CP group in tumor inhibition rate; WCPP-G, WCPP-Z and WCPP-D groups were higher than CP group in tumor inhibition rate. Conclusion: The polysaccharide extracted from WenXian DangShen could enhance anti-tumor effects of cyclophosphamide of the mice, which might be related to improving the levels of IL-2 in peripheral blood of tumor-bearing mice, decreasing the levels of IL-4 to reverse Th1/Th2 shifting, promoting the activation and proliferation of immunological cells, arousing immunological cells' specific and nonspecific killings to tumor cells.

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备注/Memo

备注/Memo:
收稿日期：2015-12-21*基金项目：甘肃省自然科学基金资助项目(编号1308RJZA101)；甘肃省中医药科学技术研究资助项目(编号GZK-2011-73)。作者简介：吴红梅(1965—)，女，硕士研究生，副主任医师，高级讲师。研究方向：中西医结合临床研究。△通讯作者：胡芳弟(1971—)，女，教授。研究方向：中药化学分析。
更新日期/Last Update: 2016-04-15