[1]李素明,杭柏亚△.痹痛合剂对OA大鼠关节软骨形态学变化及软骨细胞Bcl-2基因表达的影响[J].西部中医药,2016,29(05):18-22.
 LI Suming,HANG Baiya.The Influence of BiTong Mixture on the Expression of Cartilage Cells Bcl-2 and Articular Cartilage Morphological Changes of OA Rats[J].Western Journal of Traditional Chinese Medicine,2016,29(05):18-22.
点击复制

痹痛合剂对OA大鼠关节软骨形态学变化及软骨细胞Bcl-2基因表达的影响()
分享到:

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
29
期数:
2016年05期
页码:
18-22
栏目:
方药·药理研究
出版日期:
2016-05-15

文章信息/Info

Title:
The Influence of BiTong Mixture on the Expression of Cartilage Cells Bcl-2 and Articular Cartilage Morphological Changes of OA Rats
文章编号:
1004-6852(2016)05-0018-05
作者:
李素明杭柏亚△
南京市中医院,江苏 南京 210001
Author(s):
LI Suming HANG Baiya△
Nanjing Municipality TCM Hospital, Nanjing 210001, China
关键词:
骨性关节炎软骨细胞细胞形态学B细胞淋巴瘤/白血病-2痹痛合剂
Keywords:
osteoarthritis cartilage cells cellular morphology B cell lymphoma/leukemia-2 BiTong mixture
分类号:
R285.5
文献标志码:
A
摘要:
目的:观察痹痛合剂对骨性关节炎(OA)关节软骨细胞形态学变化的影响并探讨其可能的作用机制。方法:将SD雄性大鼠64只随机分为假手术组(A组)、模型组(B组)、硫酸氨基葡萄糖组(C组)和痹痛合剂组(D组),A组行假手术,B、C、D组采用Hulth法复制OA模型,A组常规饲养,其余各组术后分别加服生理盐水,硫酸氨基葡萄糖溶液和痹痛合剂。术后第8周处死,分别大体观察、光镜400×和电镜下观察关节软骨细胞形态学变化,原位杂交法检测关节软骨细胞中Bc1-2 mRNA表达。结果:B组关节软骨呈典型OA形态学改变,关节软骨细胞中Bc1-2 mRNA表达减弱;C、D组关节软骨轻度退变,形态学方面与A组接近,关节软骨细胞中Bc1-2 mRNA表达显著增强,与B组比较差异有统计学意义(P<0.01)。结论:痹痛合剂与硫酸氨基葡萄糖能延缓OA大鼠关节软骨的退变,其可能的作用机制是通过提高关节软骨细胞中Bc1-2基因的表达,抑制关节软骨细胞的凋亡。
Abstract:
Objective: To observe the influence of BiTong mixture on articular cartilage morphology of osteoarthritis(OA) rats, and explore its possible mechanism. Methods: Sixty-four SD male rats were randomized into sham operation group(A), the model group (B), glucosamine sulfate group (C) and BiTong mixture group (D), the rats in group A were performed with sham operation, the rats in the group B, C and D were replicated by Hulth method, the rats in the group A accepted conventional feeding, the rats in other groups took physiological saline, glucosamine sulfate solution and BiTong mixture after the operation respectively. The rats were sacrificed at the eighth week after the operation, cellular morphology of articular cartilage was inspected by gross observation, under light microscope and electron microscope, and the expressions of Bcl-2 mRNA in articular cartilage cells were detected by in situ hybridization(ISH). Results: Articular cartilage showed typical OA morphological changes in the group B, the expressions of Bcl-2 mRNA in articular cartilage cells reduced obviously; articular cartilage degenerated slightly in the groups C and D, morphological changes were similar to the rats in the group A, the expressions of Bcl-2 mRNA in articular cartilage cells enhanced significantly, it had significant difference compared with the group B(P<0.01). Conclusion: BiTong mixture and glucosamine sulfate solution could delay articular cartilage degeneration of OA rats, and the mechanism might be inhibiting articular cartilage cellular apoptosis by raising the expressions of Bcl-2 mRNA in articular cartilage cells.

相似文献/References:

[1]田雪梅,张丽君,柳树英.中医外治法辨证治疗膝骨性关节炎120例[J].西部中医药,2012,25(10):95.
 TIAN Xue-mei,ZHANG Li-jun,LIU Shu-ying.Syndrome Differentiation of TCM External Treatment for 120 Cases of Knee Osteoarthritis[J].Western Journal of Traditional Chinese Medicine,2012,25(05):95.
[2]赵道洲,郑恒恒.手法配合陇中Ⅰ号汽雾透皮疗法治疗膝骨性关节炎的临床研究*[J].西部中医药,2013,26(04):36.
 ZHAO Daozhou,ZHENG Hengheng.Clinical Study on Manipulation and Gansu Mist Transdermal Therapy No.1 in Treating Knee Osteoarthritis[J].Western Journal of Traditional Chinese Medicine,2013,26(05):36.
[3]李兴勇,徐霞,麻虎,等.中医药治疗膝关节骨性关节炎的现状[J].西部中医药,2013,26(12):144.
 LI Xingyong,XU Xia,MA Hu,et al.Progress of Treatment for Knee Osteoarthritis by TCM[J].Western Journal of Traditional Chinese Medicine,2013,26(05):144.
[4]陆金金,欧阳八四.电针与温针灸治疗瘀血阻滞型膝骨关节炎的疗效比较[J].西部中医药,2014,27(04):119.
 LU Jinjin,OUYANG Basi.Comparison of Clinical Effects between Acusector and Warm Needling Moxibustion in Treating Knee Osteoarthritis of Blood Stasis Obstruction Pattern[J].Western Journal of Traditional Chinese Medicine,2014,27(05):119.
[5]李春娥,董建文,王文波.膝关节骨性关节炎熏洗方用药规律文献研究[J].西部中医药,2015,28(03):97.
[6]王想福,石瑞芳,武纪玲,等.玻璃酸钠单独使用与联合解毒消瘀膏治疗膝骨性关节炎疗效观察[J].西部中医药,2015,28(03):118.
[7]周天恒.针灸治疗膝骨性关节炎临床观察[J].西部中医药,2015,28(04):123.
[8]李宁,李应福,谢兴文,等.兰州地区膝关节骨性关节炎的中医证候分布特点[J].西部中医药,2016,29(02):95.
 LI Ning,LI Yingfu,XIE Xingwen,et al.Distribution Features of TCM Syndromes of Knee Osteoarthritis in Lanzhou City[J].Western Journal of Traditional Chinese Medicine,2016,29(05):95.
[9]姚兴璋,李兴勇,李喜香,等.骨痹止痛液对兔膝骨性关节炎软骨中相关因子表达影响的研究[J].西部中医药,2016,29(04):29.
 YAO Xingzhang,LI Xingyong,LI Xixiang,et al.Study on the Influence of GuBi ZhiTong Liquid on Cartilage-related Factors Expressions of the Rabbits with Knee Osteoarthritis[J].Western Journal of Traditional Chinese Medicine,2016,29(05):29.
[10]李莉红,孙士杰,张选平,等.耳针与灸法并用对膝骨性关节炎患者Lysholm评分及VAS积分的影响[J].西部中医药,2016,29(04):109.
 LI Lihong,SUN Shijie,ZHANG Xuanping,et al.Influence of Ear Acupuncture and Moxibustion on Lysholm Scales and VAS of the Patients with Knee Osteoarthritis[J].Western Journal of Traditional Chinese Medicine,2016,29(05):109.

备注/Memo

备注/Memo:
收稿日期:2015-05-10*基金项目:南京市医学科技发展项目(编号YKK12151)。作者简介:李素明(1975—),男,硕士学位,副主任医师,副教授。研究方向:骨与关节疾病的中西医结合治疗。△通讯作者:杭柏亚(1965—),男,硕士研究生导师,主任医师。研究方向:骨关节疾病的中西医结合治疗。
更新日期/Last Update: 2016-05-15