[1]程涛,杨丽霞,李钦,等.镰形棘豆总黄酮含药血清对TGF-β1诱导的人肾小管上皮细胞VEGF mRNA表达的影响[J].西部中医药,2017,30(01):4-6.
　CHENG Tao,YANG Lixia,LI Qin,et al.Effects of Drug Serum of Total Flavonoids of Oxytropis Falcate on VEGF mRNA Expression in Human Renal Tubular Epithelial Cell Induced by TGF-β1[J].Western Journal of Traditional Chinese Medicine,2017,30(01):4-6.
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镰形棘豆总黄酮含药血清对TGF-β1诱导的人肾小管上皮细胞VEGF mRNA表达的影响()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
30
期数:
2017年01期
页码:
4-6
栏目:
出版日期:
2017-01-15

文章信息/Info

Title:
Effects of Drug Serum of Total Flavonoids of Oxytropis Falcate on VEGF mRNA Expression in Human Renal Tubular Epithelial Cell Induced by TGF-β1
文章编号:
1004-6852(2017)01-0004-03
作者:
程涛1，杨丽霞1，李钦2，李晓东1，姜华1，刘铜华3
1 甘肃省中医药研究院，甘肃 兰州730050； 2 甘肃省中医学校； 3 北京中医药大学
Author(s):
CHENG Tao1, YANG Lixia1, LI Qin2, LI Xiaodong1, JIANG Hua1, LIU Tonghua3
1 Gansu Provincial Academy of Chinese Medicine, Lanzhou 730050, China; 2 Gansu Provincial School of Traditional Chinese Medicine; 3 Beijing University of Chinese Medicine
关键词:
镰形棘豆总黄酮; 转化生长因子β1; 人肾小管上皮细胞; 血管内皮生长因子
Keywords:
total flavonoids of oxytropis falcata;  TGF-β1;  human renal tubular epithelial cell;  VEGF
分类号:
R285
文献标志码:
A
摘要:
目的：通过观察镰形棘豆总黄酮含药血清干预转化生长因子β1（TGF-β1）诱导的人肾小管上皮细胞（HK-2）纤维化细胞因子——血管内皮生长因子（VEGF）mRNA的表达，进一步探讨其在防治肾间质纤维化方面的作用。方法：将HK-2 细胞用含10%胎牛血清的DMEM/F12（1∶1）培养基培养；实验分为4 组：空白对照组、TGF-β1诱导组（TGF-β110 ng/mL）、空白血清对照组（TGF-β110 ng/mL +10%空白血清）、镰形棘豆总黄酮组（TGF-β110 ng/mL+10%镰形棘豆总黄酮含药血清）。药物干预24小时后，荧光定量PCR检测VEGF mRNA的表达。结果：HK-2细胞经TGF-β1诱导后，VEGF mRNA的表达显著上升，与空白对照组比较差异有统计学意义（P＜0.05），经镰形棘豆总黄酮药物血清干预后，VEGF mRNA的表达逐步下降，与单纯TGF-β1诱导组比较差异有统计学意义（P＜0.05）。结论：镰形棘豆总黄酮在一定程度上能够抑制TGF-β1诱导的人肾小管上皮细胞纤维化，其机制可能与调节纤维化细胞因子VEGF的mRNA表达有关。
Abstract:
Objective: To explore the effects of preventing and treating renal interstitial fibrosis of total flavonoids of oxytropis falcata further by observing the intervention effects on HK-2 and (VEGF) mRNA expressions induced by TGF-β1. Methods: HK-2 cell was cultivated by using DMEM/F12(1∶1) medium containing 10% fetal bovine serum; the experiments were divided into four groups: blank control group, TGF-β1 induced group (TGF-β1 10 ng/mL), blank serum control group (TGF-β110 ng/mL +10% blank serum), total flavonoids of oxytropis falcate(TGF-β110 ng/mL+10%drug serum of total flavonoids of oxytropis falcata ). After 24 hours of drug intervention, VEGF mRNA expression was detected by fluorescent quantitative PCR. Results: After HK-2 cell was induced by TGF-β1, VEGF mRNA expression increased obviously, there was statistical difference compared with blank control group(P＜0.05), after intervention of drug serum of total flavonoids of oxytropis falcata, VEGF mRNA expression decreased gradually, there was statistical differences compared with simple TGF-β1 induced group(P＜0.05). Conclusion: Total flavonoids of oxytropis falcate can inhibit human renal tubular epithelial cell fibrosis induced by TGF-β1 to a certain degree, and its mechanism might be related to fibrotic VEGF mRNA expression regulation.

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备注/Memo

备注/Memo:
收稿日期：2016-06-20 *基金项目：甘肃省中医药管理局科研课题(编号GZK-2015-32)。作者简介：程涛(1971—)，男，副主任医师。研究方向：中医内科学及中医药信息学。
更新日期/Last Update: 2017-01-15