[1]李彩东,王信,潘新波,等.波长切换技术同时测定加味小柴胡汤胶囊中党参炔苷等4种成分[J].西部中医药,2017,30(01):26-28.
 LI Caidong,WANG Xin,PAN Xinbo,et al.Detection of Four Kinds of Ingredients in Modified Xiao ChaiHu Capsules by HPLC Wavelength Switching Method Simultaneously[J].Western Journal of Traditional Chinese Medicine,2017,30(01):26-28.
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波长切换技术同时测定加味小柴胡汤胶囊中党参炔苷等4种成分()
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《西部中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
30
期数:
2017年01期
页码:
26-28
栏目:
方药·质量分析
出版日期:
2017-01-15

文章信息/Info

Title:
Detection of Four Kinds of Ingredients in Modified Xiao ChaiHu Capsules by HPLC Wavelength Switching Method Simultaneously
文章编号:
1004-6852(2017)01-0026-03
作者:
李彩东王信潘新波张伟
兰州市第二人民医院,甘肃 兰州 730046
Author(s):
LI Caidong WANG Xin PAN Xinbo ZHANG Wei
The Second People′s Hospital of Lanzhou City, Lanzhou 730046, China
关键词:
HPLC波长切换法加味小柴胡胶囊甘草苷党参炔苷黄芩苷黄芩素
Keywords:
HPLC wavelength switching method modified XiaoChaiHu capsules liquiritin lobetyolin baicalin baicalein
分类号:
R285.1
文献标志码:
A
摘要:
目的:运用安捷伦1260高效液相色谱仪,建立多波长切换技术,同时测定加味小柴胡胶囊中党参炔苷、甘草苷、黄芩苷、黄芩素含量的方法。方法:采用菲罗门ODS C18柱,以乙腈(流动相A)-0.1%磷酸水溶液为流动相体系,梯度洗脱(0~40分钟,乙腈20%~45%),流速1.0 mL/min,一般波长254 nm,检测波长在每个成分出峰的时间段内切换为该成分最大吸收波长。结果:甘草苷、党参炔苷、黄芩苷、黄芩素分别在0.05~0.75、0.042~0.63、0.80~12.0、0.04~0.60 μg范围内线性关系良好。相关系数分别为0.9995、0.9992、0.9999和0.9997,平均加样回收率分别为100.31%、98.92%、101.06%、100.17%,RSD%分别为1.90、1.63、2.21、1.19。结论:本研究建立的方法稳定可靠,便于操作,可以解决党参炔苷因含量较低无法检测的问题。
Abstract:
Objective: To establish wavelength switching method for detecting the contents of lobetyolin, liquiritin, baicalin and baicalein contained in modified XiaoChaiHu capsules by using HPLC (Agilent 1260) simultaneously. Methods: HPLC separation was performed on Phenomenex C18(4.6 mm × 250 mm, 5 μm), acetonitrile-0.1% phosphoric acid(0~40 min, acetonitrile 20%~45% ) as mobile phase, and the flow rate was 1.0 mL/min,the wavelength were maximum absorption wavelength according to every component respectively. Results: Liquiritin, lobetyolin, baicalin and baicalein showed good linear relationship in the range between 0.05~0.75μg, 0.042~0.63 μg, 0.80~12.0 μg and 0.04~0.60 μg respectively. Correlation coefficients were 0.9995, 0.9992, 0.9999 and 0.9997, average recovery rates were 100.31%, 98.92%, 101.06% and 100.17%, RSD% were 1.90, 1.63, 2.21 and 1.19. Conclusion: The method, stable, reliable and easy to operate, could solve the problem that lobetyolin is too low to detect.

备注/Memo

备注/Memo:
收稿日期:2016-02-23 *基金项目:2013年兰州市第三批科技计划项目(编号2013-3-54)。作者简介:李彩东(1969—),女,博士学位,主任药师。研究方向:药物制剂和药物分析。
更新日期/Last Update: 2017-01-15