波长切换技术同时测定加味小柴胡汤胶囊中党参炔苷等4种成分-《西部中医药》

文章信息/Info

Title:: Detection of Four Kinds of Ingredients in Modified Xiao ChaiHu Capsules by HPLC Wavelength Switching Method Simultaneously

Author(s):: LI Caidong, WANG Xin, PAN Xinbo, ZHANG Wei; The Second People′s Hospital of Lanzhou City, Lanzhou 730046, China

Keywords:: HPLC wavelength switching method; modified XiaoChaiHu capsules; liquiritin; lobetyolin; baicalin; baicalein

摘要:: 目的：运用安捷伦1260高效液相色谱仪，建立多波长切换技术，同时测定加味小柴胡胶囊中党参炔苷、甘草苷、黄芩苷、黄芩素含量的方法。方法：采用菲罗门ODS C18柱，以乙腈(流动相A)-0.1%磷酸水溶液为流动相体系，梯度洗脱(0～40分钟，乙腈20%～45%)，流速1.0 mL/min，一般波长254 nm，检测波长在每个成分出峰的时间段内切换为该成分最大吸收波长。结果：甘草苷、党参炔苷、黄芩苷、黄芩素分别在0.05～0.75、0.042～0.63、0.80～12.0、0.04～0.60 μg范围内线性关系良好。相关系数分别为0.9995、0.9992、0.9999和0.9997，平均加样回收率分别为100.31%、98.92%、101.06%、100.17%，RSD%分别为1.90、1.63、2.21、1.19。结论:本研究建立的方法稳定可靠，便于操作，可以解决党参炔苷因含量较低无法检测的问题。

Abstract:: Objective: To establish wavelength switching method for detecting the contents of lobetyolin, liquiritin, baicalin and baicalein contained in modified XiaoChaiHu capsules by using HPLC (Agilent 1260) simultaneously. Methods: HPLC separation was performed on Phenomenex C18(4.6 mm × 250 mm, 5 μm), acetonitrile-0.1% phosphoric acid(0~40 min, acetonitrile 20%~45% ) as mobile phase, and the flow rate was 1.0 mL/min，the wavelength were maximum absorption wavelength according to every component respectively. Results: Liquiritin, lobetyolin, baicalin and baicalein showed good linear relationship in the range between 0.05~0.75μg, 0.042~0.63 μg, 0.80~12.0 μg and 0.04～0.60 μg respectively. Correlation coefficients were 0.9995, 0.9992, 0.9999 and 0.9997, average recovery rates were 100.31%, 98.92%, 101.06% and 100.17%, RSD% were 1.90, 1.63, 2.21 and 1.19. Conclusion: The method, stable, reliable and easy to operate, could solve the problem that lobetyolin is too low to detect.