[1]周昱,帕丽达·阿不力孜△,依力奴尔·艾尼瓦尔,等.阿里红多糖体外免疫活性的研究[J].西部中医药,2017,30(08):5-7.
　ZHOU Yu,Palida·ABULIZI,Yilinuer·AINIWAER,et al.Study on Immune Activity of Fomes Oficinalis Sporophore Polysaccharide in Vitro[J].Western Journal of Traditional Chinese Medicine,2017,30(08):5-7.
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阿里红多糖体外免疫活性的研究
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
30
期数:
2017年08期
页码:
5-7
栏目:
出版日期:
2017-08-15

文章信息/Info

Title:
Study on Immune Activity of Fomes Oficinalis Sporophore Polysaccharide in Vitro
文章编号:
1004-6852(2017)08-0005-03
作者:
周昱，帕丽达·阿不力孜△，依力奴尔·艾尼瓦尔，丛媛媛，米仁沙·牙库甫
新疆医科大学药学院，新疆 乌鲁木齐 830011
Author(s):
ZHOU Yu, Palida·ABULIZI△, Yilinuer·AINIWAER, CONG Yuanyuan, Mirensha·YAKUFU
Pharmacy College of Xinjiang Medical University, Urumqi 830011, China
关键词:
阿里红; 多糖; 淋巴细胞; 免疫调节
Keywords:
fomes oficinalis sporophore;  polysaccharide;  lymphocyte;  immunoregulation
分类号:
R96
文献标志码:
A
摘要:
目的：探讨阿里红多糖（FoP）对小鼠体外免疫的调节作用。方法：采用体外淋巴细胞增殖实验考察FoP对小鼠脾淋巴细胞增殖的影响；采用磺酰罗丹明B(SRB)法检测不同浓度FoP单独作用及其与有丝分裂原ConA、LPS共同作用对小鼠脾淋巴细胞增殖的影响；采用酶联免疫吸附实验(ELSA)法检测不同浓度FoP体外刺激对小鼠脾淋巴细胞诱生白介素2(IL-2)、白介素13(IL-13)、肿瘤坏死因子α(TNF-α)等细胞因子的影响。结果：Fop在25～100μg/mL浓度范围内能显著促进小鼠脾淋巴细胞增殖，并与ConA、LPS协同促进T、B淋巴细胞增殖，还能诱导脾淋巴细胞分泌IL-2、TNF-α。结论：FoP具有免疫促进作用。
Abstract:
Objective: To explore the immunoregulation of fomes oficinalis sporophore polysaccharide (FoP) on the mice in vitro. Methods: Lymphocyte proliferation test was adopted to investigate the effects of FoP on the proliferation of splenic lymphocyte of the mice; and SRB method was used to detect the act of FoP independently in different concentrations and its combined action with the mitogen ConA, LPS on splenic lymphocyte of the mice; ELISA method was adopted to survey the effects of FoP with different concentrations in vitro stimulating splenic lymphocyte inducing the cell factors such as IL-2, IL-13, TNF-α and others. Results: FoP could promote the proliferation of splenic lymphocyte of the mice in the concentrations ranged between 25 and 100 μg/mL, and promote T and B lymphocyte proliferation by working with ConA, LPS, and induce splenic lymphocyte secreting IL-2 and TNF-α. Conclusion: FoP plays a role in immunological enhancement.

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备注/Memo

备注/Memo:
收稿日期：2017-02-25 *基金项目：国家科学自然基金(编号81460635)。 作者简介：周昱(1990—)，女，硕士学位。研究方向：维吾尔药药效物质基础研究。 △通讯作者：帕丽达·阿不力孜(1962—)，女，博士学位，教授。研究方向：维吾尔药药效物质基础研究。
更新日期/Last Update: 2017-08-15