[1]李钦,范强,胡继宏,等.富硒黄芪提取物对免疫抑制大鼠细胞因子IL-2、IL-4、IFN-γ及TNF-α的影响[J].西部中医药,2018,31(03):22-25.
　LI Qin,FAN Qiang,HU Jihong,et al.The Effects of Extract of Selenium-enriched Astragalus Membranaceus on Cytokines IL-2, IL-4, IFN-γ and TNF-α of Immunosuppressive Rats[J].Western Journal of Traditional Chinese Medicine,2018,31(03):22-25.
点击复制

富硒黄芪提取物对免疫抑制大鼠细胞因子IL-2、IL-4、IFN-γ及TNF-α的影响()
分享到：

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
31
期数:
2018年03期
页码:
22-25
栏目:
出版日期:
2018-03-15

文章信息/Info

Title:
The Effects of Extract of Selenium-enriched Astragalus Membranaceus on Cytokines IL-2, IL-4, IFN-γ and TNF-α of Immunosuppressive Rats
文章编号:
1004-6852(2018)03-0022-04
作者:
李钦1，范强2，胡继宏2，高博1，宋爽1，李海东3，杨丽霞4△
1 甘肃卫生职业学院，甘肃 兰州 730000； 2 甘肃中医药大学； 3 甘肃省渭源县康荣中药材科技有限公司； 4 甘肃省中医药研究院
Author(s):
LI Qin1, FAN Qiang2, HU Jihong2, GAO Bo1, SONG Shuang1, LI Haidong3, YANG Lixia4△
1 Gansu Health Vocational College, Lanzhou 730000, China; 2 Gansu University of Chinese Medicine; 3 Kangrong Chinese Medicine Technology Limited Company; 4 Gansu Provincial Academy of Chinese Medicine
关键词:
免疫抑制; 细胞因子; 环磷酰胺; 富硒黄芪提取物; 大鼠
Keywords:
immunosuppression;  cytokines;  cyclophosphamide;  extract of selenium-enriched Astragalus membranaceus;  rats
分类号:
R285
摘要:
目的：观察富硒黄芪提取物对免疫抑制大鼠细胞因子白细胞介素2(IL-2)、白细胞介素(IL-4)、干扰素γ(IFN-γ)及肿瘤坏死因子α(TNF-α)的影响。方法：将SD大鼠随机分为6组，空白组、模型组及富硒黄芪提取物高、中、低剂量组和普通黄芪提取物组。除空白组外，其余5组均采用腹腔注射环磷酰胺构建大鼠免疫抑制模型。造模成功后分组干预治疗。给药第7天后，各组大鼠禁食8小时，股动脉取血，采用酶联免疫吸附法(ELISA)检测血清中IL-2、IL-4、IFN-γ及TNF-α的含量。取出胸腺、脾脏记录其重量变化。结果：与空白组比较，模型组大鼠胸腺、脾脏指数显著降低，差异有统计学意义(P＜0.05)；与模型组比较，各干预组大鼠胸腺、脾脏指数有所升高，差异有统计学意义(P＜0.05)。与空白组比较，模型组大鼠细胞因子(IL-2、IL-4、IFN-γ)含量显著降低，TNF-α含量显著升高，差异有统计学意义(P＜0.05)；与模型组比较，各干预组大鼠IL-2、IL-4、IFN-γ含量有所升高，TNF-α含量有所降低，差异有统计学意义(P＜0.05)。结论：富硒黄芪提取物能显著提高环磷酰胺所致免疫抑制模型大鼠的免疫功能，且这种作用较普通黄芪更为显著。
Abstract:
Objective: To observe the effects of extract of selenium-enriched Astragalus membranaceus on cytokines IL-2, IL-4, IFN-γ and TNF-α in immunosuppressive rats. Methods: SD rats were randomly divided into six groups: the blank group, the model group, the high, middle and low dose groups of extract of selenium-enriched Astragalus membranaceus as well as the common astragalus extract group. Other five groups were injected intraperitoneally with cyclophosphamide to construct the immunosuppressive models except the blank group. After the modeling, the groups were intervened respectively. After seven days of treatment, rats, fasted for eight hours, were blooded from the femoral artery. The contents of cytokines IL-2, IL-4, IFN-γ and TNF-α in the serum of immunosuppressive rats were detected by enzyme-linked immunosorbent assay ELISA, thymus gland and the spleen were taken out and the changes were recorded. Results: Compared with the blank group, thymus and spleen indexes in the model group decreased significantly, and the difference was statistically significant(P＜0.05); Compared with the model group, thymus and spleen indexes of the intervention groups increased, and the difference was statistically significant(P＜0.05). And compared with the blank group, the contents of cytokines IL-2, IL-4 and IFN-γ of the rats in the model group decreased significantly, the contents of TNF-α raised notably, and the differences were statistically significant (P＜0.05); Furthermore, compared with the model group, the contents of cytokines IL-2, IL-4 and IFN-γ of the rats in the intervention groups raised significantly, the contents of TNF-α decreased, the difference was statistically significant (P＜0.05). Conclusion: Extract of selenium-enriched Astragalus membranaceus can significantly improve the immune function of immunosuppressive rat model induced by cyclophosphamide and the effects are more significant than common astraguls membranaceus.

相似文献/References:

[1]李雪嫣,张李峰,桂曼曼,等.复方玉屏风散对小鼠细胞免疫力的增强作用研究[J].西部中医药,2013,26(08):11.
　LI Xueyan,ZHANG Lifeng,GUI Manman,et al.Study on Compound YuPingFengSan Oral Liquid Improving Cellular Immunity of Mice[J].Western Journal of Traditional Chinese Medicine,2013,26(03):11.
[2]任莉莉,问明,季静,等.参附注射液联合细胞因子对人慢性髓性白血病细胞来源树突状细胞诱导生成的影响[J].西部中医药,2014,27(07):4.
　REN Lili,WEN Ming,JI Jing,et al.Influence of ShenFu Injection Combined with Cytokines on the Induction and Generation of Dendritic Cells in the Patients with Chronic Myelogenous Leukemia[J].Western Journal of Traditional Chinese Medicine,2014,27(03):4.
[3]马琼瑛.甲硝唑联合桂枝茯苓丸治疗慢性盆腔炎 60 例[J].西部中医药,2014,27(08):101.
[4]谢兴文,徐世红,李晶,等.骨肉瘤相关细胞因子水平的诊断与测定[J].西部中医药,2015,28(10):147.
[5]王永宏.风湿汤联合风湿足浴粉浴足对膝关节骨性关节炎相关细胞因子表达的影响[J].西部中医药,2017,30(03):96.
　WANG Yonghong.The Influence of FengShi Tang Combined with Foot Bath with Rheumatoid Foot Powder on the Expressions of Cytokines Related to Knee Osteoarthritis[J].Western Journal of Traditional Chinese Medicine,2017,30(03):96.
[6]张文进,孙志华,李永吉,等.丹参注射液对膝骨性关节炎关节液及炎症血清细胞因子水平的影响[J].西部中医药,2019,32(02):21.
　ZHANG Wenjin,SUN Zhihua,LI Yongji,et al.Influence of DanShen Injection on the Level of Serum Cytokines and Joint Fluid of Knee Osteoarthritis[J].Western Journal of Traditional Chinese Medicine,2019,32(03):21.
[7]滕雅娟,张燕,谭志敏,等.丹参酮联合优甲乐对桥本甲状腺炎伴甲状腺功能减退患者免疫功能的影响[J].西部中医药,2019,32(03):102.
　TENG Yajuan,ZHANG Yan,TAN Zhimin,et al.Effects of Tanshinone and Euthyrox on Immunologic Function of Hashimoto's Thyroiditis Accompanying Hypothyroidism[J].Western Journal of Traditional Chinese Medicine,2019,32(03):102.
[8]庄靖卿,赵海玲△.经络氧疗法联合穴位按摩对颈源性眩晕抑郁患者的影响[J].西部中医药,2019,32(05):97.
　ZHUANG Jingqing,ZHAO Hailing.Influence of Meridian Oxygen Therapy Combined with Acupoint Massage on the Patients with Cervical Vertigo and Depression[J].Western Journal of Traditional Chinese Medicine,2019,32(03):97.
[9]胡刚明,万彬彬,张成国,等.穴位自血疗法联合盐酸左西替利嗪分散片治疗慢性荨麻疹临床观察[J].西部中医药,2022,35(07):95.[doi:10.12174/j.issn.2096-9600.2022.07.25]
　HU Gangming,WAN Binbin,ZHANG Chenguo,et al.Clinical Observation on Acupoint Self-blood Therapy and Levocetirizine Hydrochloride Tablets in the Treatment of Chronic Urticaria[J].Western Journal of Traditional Chinese Medicine,2022,35(03):95.[doi:10.12174/j.issn.2096-9600.2022.07.25]
[10]胡金霞,杨帆,卢军,等.景衣安神散穴位贴敷对失眠大鼠行为学及相关细胞因子的影响?[J].西部中医药,2021,34(11):41.[doi:10.12174/j.issn.2096-9600.2021.11.09]
　HU Jinxia,YANG Fan,LU Jun,et al.Effects of Jingyi Anshen Powder Acupoint Application on Behavior and the Related Cytokines in Rats with Insomnia[J].Western Journal of Traditional Chinese Medicine,2021,34(03):41.[doi:10.12174/j.issn.2096-9600.2021.11.09]
[11]胡君茹,姜华△.镰形棘豆黄酮苷元对免疫抑制小鼠细胞因子的影响[J].西部中医药,2017,30(11):12.
　HU Junru,JIANG Hua.The Effects of Flavonoid Glycosides Extracted from LianXing JiDou on the Cytokines of the Immunosuppressive Mice[J].Western Journal of Traditional Chinese Medicine,2017,30(03):12.

备注/Memo

备注/Memo:
收稿日期：2016-11-29 作者简介：李钦(1979—)，男，硕士学位，高级讲师。研究方向：糖尿病及其并发症的中医药防治。 △通讯作者：杨丽霞(1979—)，女，博士学位，副主任医师。研究方向：糖尿病及并发症的中医药防治。
更新日期/Last Update: 2018-03-15