[1]焦正花,李文萍,赵凤菊,等.清热解毒冲洗液质量标准研究[J].西部中医药,2018,31(08):16-19.
 JIAO Zhenghua,LI Wenping,ZHAO Fengju,et al.Study on Quality Standard of Heat-clearing Detoxifying Flush Fluid[J].Western Journal of Traditional Chinese Medicine,2018,31(08):16-19.
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清热解毒冲洗液质量标准研究()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
31
期数:
2018年08期
页码:
16-19
栏目:
方药·质量分析
出版日期:
2018-08-15

文章信息/Info

Title:
Study on Quality Standard of Heat-clearing Detoxifying Flush Fluid
文章编号:
1004-6852(2018)08-0016-04
作者:
焦正花1李文萍1赵凤菊2禚君1
1 甘肃省中医院,甘肃 兰州 730050; 2 甘肃省肿瘤医院
Author(s):
JIAO Zhenghua1 LI Wenping1 ZHAO Fengju2 ZHUO Jun1
1 Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China; 2 Gansu Provincial Cancer Hospital
关键词:
清热解毒冲洗液薄层色谱高效液相质量控制
Keywords:
heat-clearing detoxifying flush fluid thin-layer chromatography HPLC quality standard
分类号:
R932
文献标志码:
A
摘要:
目的:建立清热解毒冲洗液的质量控制方法。方法:采用薄层色谱法对方中当归、苦参、大黄、赤芍进行定性鉴别,采用高效液相色谱法测定清热解毒冲洗液中大黄有效成分大黄酸、大黄素、大黄酚的含量。结果:薄层色谱特征斑点清晰,专属性强,阴性对照无干扰;高效液相色谱法测定大黄酸在0.056~0.56 μg范围内线性关系良好(r=0.999 8,n=5),平均回收率为98.47%(RSD=0.77%,n=5);大黄素在0.179~1.79 μg范围内线性关系良好(r=0.999 7,n=5),平均回收率为98.58%(RSD=0.51%,n=5);大黄酚在0.035~0.35 μg范围内线性关系良好(r=0.999 0,n=5),平均回收率为98.63%(RSD=0.66%,n=5)。结论:该方法简便可靠、专属性强、重复性好,可用于该制剂的质量控制。
Abstract:
Objective: To establish the method for quality control of heat-clearing detoxifying flush fluid. Methods: DangGui (Angelica sinensis), kuh-seng, DaHuang (Rheum palmatum L.) and ChiShao (Radix Paeoniae Rubra) in the prescription were identified qualitatively by thin-layer chromatography(TLC), the contents of the active ingredients of DaHuang including chrysophanic acid, emodin and rheic acid were measured by HPLC. Results: TLC was featured by clear spots, strong specificity, no interference with negative controls; the results of HPLC showed that chrysophanic acid had good linear relationship in the range between 0.056~0.56 μg (r=0.999 8, n=5), average recovery rate was 98.47% (RSD=0.77%, n=5); emodin presented good linear relationship in the range between 0.179~1.79 μg (r=0.999 7, n=5), average recovery rate was 98.58% (RSD=0.51%, n=5); rheic acid demonstrated good linear relationship in the range between 0.035~0.35 μg(r=0.999 0, n=5), average recovery rate was 98.63% (RSD=0.66%, n=5). Conclusion: The method, simple, convenient, reliable, specific and reproducible, could be used for quality control of the preparation.

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备注/Memo

备注/Memo:
收稿日期:2018-02-27 *基金项目:甘肃省中医药管理局科研课题(编号GZK-2016-12)。 作者简介:焦正花(1969—),女,副主任药师,研究方向:中药制剂研发。
更新日期/Last Update: 2018-08-15