[1]魏玉娇,何玲,郭小荣,等.髓芯减压联合生骨再造丸对兔激素性股骨头坏死骨组织BMP-2与VEGF mRNA的影响[J].西部中医药,2018,31(09):34-37.
　WEI Yujiao,HE Ling,GUO Xiaorong,et al.Impact of Core Decompression and ShengGu ZaiZao Pills on BMP-2 and VEGF mRNA of Bone Tissue of Rabbit with Steroid-induced Femoral Head Necrosis[J].Western Journal of Traditional Chinese Medicine,2018,31(09):34-37.
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髓芯减压联合生骨再造丸对兔激素性股骨头坏死骨组织BMP-2与VEGF mRNA的影响()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
31
期数:
2018年09期
页码:
34-37
栏目:
出版日期:
2018-09-15

文章信息/Info

Title:
Impact of Core Decompression and ShengGu ZaiZao Pills on BMP-2 and VEGF mRNA of Bone Tissue of Rabbit with Steroid-induced Femoral Head Necrosis
文章编号:
1004-6852(2018)09-0034-04
作者:
魏玉娇1，何玲2，郭小荣1，曹林忠2，张虎林1，张晓刚1，郭成龙1△
1 甘肃中医药大学附属医院，甘肃 兰州 730020； 2 甘肃中医药大学
Author(s):
WEI Yujiao1, HE Ling2, GUO Xiaorong1, CAO Linzhong2, ZHANG Hulin1, ZHANG Xiaogang1, GUO Chenglong1△
1 Affiliated Hospital of Gansu University of Chinese Medicine, Lanzhou 730020, China; 2 Gansu University of Chinese Medicine
关键词:
股骨头坏死; 激素性; BMP-2; VEGF; 髓芯减压术; 生骨再造丸
Keywords:
steroid-induced femoral head necrosis;  BMP-2;  VEGF;  core decompression;  ShengGu ZaiZao pills
分类号:
R285.5
文献标志码:
A
摘要:
目的：探讨髓芯减压联合生骨再造丸对激素性家兔坏死股骨头骨形成蛋白2(BMP-2)与血管内皮生长因子mRNA(VEGF mRNA)的影响。方法：将成年雄性青紫兰兔60只随机分为5组，正常对照组（N）8只，模型对照组（Y）、生骨再造丸组（M）、手术组（S）、综合治疗组（MS），每组13只。Y、M、S、MS组采用内毒素加激素联合造模，造模4周后每组随机处死1只兔子，检验造模是否成功。造模成功后N组和Y组灌服生理盐水20 mL/d，M组灌服生骨再造丸溶液4.6 mL/(kg·d)，S组采用髓芯减压术治疗，MS组在手术治疗基础上灌服同量生骨再造丸溶液，各组分别干预8周，处死动物所获股骨头行HE染色，采用RT-PCR观察骨组织中BMP-2与VEGF mRNA表达情况。结果：与Y组比较，各治疗组空陷窝率下降，BMP-2和VEGF mRNA表达水平均有不同程度升高,差异有统计学意义（P＜0.05），其中以MS组空陷窝率降低最为显著(P＜0.01）。结论：增强股骨头骨组织BMP-2和VEGF mRNA表达是髓芯减压术联合生骨再造丸治疗激素性兔股骨头坏死的作用机制之一。
Abstract:
Objective: To discuss the impacts of core decompression and ShengGu ZaiZao pills on BMP-2 and VEGF mRNA of the rabbits suffering steroid-induced femoral head necrosis. Methods: Sixty patients were randomized into five groups, eight rabbits in normal control group as N, model control group, ShengGu ZaiZao pills group, operation group and comprehensive therapy group as Y, M, S and MS, 13 rabbits each group. The rabbits in Y, M, S and MS were prepared into the models by endotoxin and hormone, in four weeks after modeling, five rabbits in each group (N,Y, M, S and MS) were sacrificed randomly to testify whether the modeling succeeded. After successfully modelling, N and Y were drenched with physiological saline, 20 mL/d, M with the solution of ShengGu ZaiZao pills, 4.6 mL/(kg·d), S accepted core decompression, MS with the solution of ShengGu ZaiZao pills in the same volume based on the operation, different groups were intervened for eight weeks respectively, femoral head of the sacrificed rabbits were performed HE staining, the expressions of VEGF and BMP-2 mRNA in bone tissue were observed by RT-PCR.Results: Compared with Y, blank pit rate of different treatment groups decreased, the expressions of BMP-2 and VEGF mRNA raised to different degrees, and the difference had statistical meaning (P＜0.05), among them, the decrease of comprehensive therapy group was the most signficant (P＜0.01). Conclusion: Enhancing the expressions of VEGF and BMP-2 mRNA in bone tissue of femoral head is one of the mechanisms of core decompression and ShengGu ZaiZao pills in the treatment for steroid-induced femoral head necrosis.

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备注/Memo

备注/Memo:
收稿日期：2017-10-27 *基金项目：甘肃省中医药管理局项目(编号GZK-2017-18)；甘肃中医药大学中青年科研基金(编号ZQ2017-8)；甘肃中医药大学附属医院院内科研及技术创新基金(编号gzfy-2017-04)。 作者简介：魏玉娇(1985—)，女，硕士学位，医师。研究方向：骨代谢的研究。 △通讯作者：郭成龙(1979—)，男，硕士学位，副主任医师，第六批全国名老中医药专家学术经验继承人，甘肃省名中医学术经验继承人。研究方向：颈肩腰腿痛及骨与关节病的中医药防治。
更新日期/Last Update: 2018-09-15