[1]隋晓丽,张红艳,田晓倩,等.肺抑瘤合剂质量标准研究[J].西部中医药,2018,31(09):46-50.
 SUI Xiaoli,ZHANG Hongyan,TIAN Xiaoqian,et al.Study on Quality Standard of Pulmonary Tumor-inhibiting Mixture[J].Western Journal of Traditional Chinese Medicine,2018,31(09):46-50.
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肺抑瘤合剂质量标准研究()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
31
期数:
2018年09期
页码:
46-50
栏目:
出版日期:
2018-09-15

文章信息/Info

Title:
Study on Quality Standard of Pulmonary Tumor-inhibiting Mixture
文章编号:
1004-6852(2018)09-0046-05
作者:
隋晓丽1张红艳1田晓倩1彭召云2李芳1刘健1郑心1△
1 山东中医药大学第二附属医院,山东 济南 250001; 2 山东中医药大学
Author(s):
SUI Xiaoli1, ZHANG Hongyan1, TIAN Xiaoqian1, PENG Zhaoyun2, LI Fang1, LIU Jian1, ZHENG Xin1△
1 The Second Affiliated Hospital to Shandong University of Traditional Chinese Medicine, Jinan 250001, China; 2 Shandong University of Traditional Chinese Medicine
关键词:
肺抑瘤合剂质量标准薄层色谱HPLC-ELSD黄芪甲苷
Keywords:
pulmonary tumor-inhibiting mixture quality standard TLC HPLC-ELSD Astragaloside A
分类号:
R282.5
文献标志码:
A
摘要:
目的:对肺抑瘤合剂进行薄层鉴别及定量测定,建立合理可行的质量控制方法与标准。方法:采用薄层色谱法(TLC)对制剂中黄芪、薏苡仁、浙贝母、甘草进行定性鉴别;建立测定肺抑瘤合剂中指标性成分黄芪甲苷的高效液相色谱-蒸发光散射检测(HPLC-ELSD)方法;采用AgilentZORBAX SB-C18色谱柱(4.6 mm×250 mm,5 μm),流动相为乙腈-水(32∶68),流速为1.0 mL/min,柱温为25℃。ELSD参数:漂移管温度为90℃,雾化温度为40℃,氮气流速为1.20 L/min。结果:TLC图斑点清晰,专属性强,能有效鉴别4味药材;黄芪甲苷进样量在0.9160~9.1600 μg范围内与峰面积线性关系良好,回归方程为lgY=1.418lgX+2.480,r=0.999 5,平均加样回收率为96.99%,RSD为0.96%(n=6)。结论:建立的鉴别和含量测定方法专属性强、重复性好、简便准确,可作为控制肺抑瘤合剂质量的方法。
Abstract:
Objective: To establish the rational and feasible method and standard of quality control by performing thin-layer chromatography (TLC) identification and quantitative determination of pulmonary tumor-inhibiting mixture. Methods: HuangQi[Astragalus membranaceus (Fisch.) Bunge.], Yiyiren (semen coicis), ZheBeiMu (Fritillaria thunbergii Miq.) and GanCao (licorice) in the preparations were identified qualitatively by TLC, and HPLC-ELSD method of indicative components Astragaloside A contained in pulmonary tumor-inhibiting mixture was established, AgilentZORBAX SB-C18 chromatographic column (4.6 mm×250 mm,5 μm) was used, acetonitrile-water (32:68) as mobile phase, flow velocity was 1.0 mL/min, column temperature was 25℃. ELSD parameters: the temperature of drift tube was 90℃, atomization temperature 40℃, nitrogen velocity was 1.20 L/min. Results: TLC spots were clear with strong specialization, and four kinds of herbs could be effectively identified; astragaloside A was in good linear relationship in the peak area and the range from 0.916 0 to 9.160 0 μg, regression equation was lgY=1.418lgX+2.480, r=0.999 5, average sample recovery was 96.99%, RSD was 0.96%(n=6). Conclusion: The established identification and content determination methods, which are specific, reproducible, simple and accurate, could be taken as the method of controlling the quality of pulmonary tumor-inhibiting mixture.

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备注/Memo

备注/Memo:
收稿日期:2017-11-10 *基金项目:山东省科技计划项目(编号2014GSF119015);济南市科技计划项目(编号201602185)。 作者简介:隋晓丽(1972—),女,硕士学位,主管药师。研究方向:中药质量标准及新药研发。 △通讯作者:郑心(1964—),女,博士研究生导师,博士学位,主任医师。研究方向:中西医结合治疗肺癌及肺间质病。
更新日期/Last Update: 2018-09-15