[1]杨彦彪,魏春华,冉广宇,等.止血通明合剂指标性成分的色谱分析[J].西部中医药,2019,32(01):32-35.
 YANG Yanbiao,WEI Chunhua,RAN Guangyu,et al.Chromatographic Analysis of Indicative Components in ZhiXue TongMing Mixture[J].Western Journal of Traditional Chinese Medicine,2019,32(01):32-35.
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止血通明合剂指标性成分的色谱分析
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
32
期数:
2019年01期
页码:
32-35
栏目:
出版日期:
2019-01-15

文章信息/Info

Title:
Chromatographic Analysis of Indicative Components in ZhiXue TongMing Mixture
文章编号:
1004-6852(2019)01-0032-04
作者:
杨彦彪魏春华冉广宇张成安张志叶
兰州市第一人民医院,甘肃 兰州 730050
Author(s):
YANG Yanbiao, WEI Chunhua, RAN Guangyu, ZHANG Cheng′an, ZHANG Zhiye
The First People′s Hospital of Lanzhou City, Lanzhou 730050, China
关键词:
薄层层析高效液相色谱分析止血通明合剂
Keywords:
TLC HPLC ZhiXue TongMing mixture
分类号:
R927.2
文献标志码:
A
摘要:
目的:定性、定量分析止血通明合剂中的指标性成分,为其质量控制提供理论依据。方法:应用Diamond ODS C18(4.6 mm×250 mm,5 μm)色谱柱,乙腈-0.1%磷酸(三乙胺调pH值至5.1)为流动相梯度洗脱,流速0.8 mL/min,柱温30℃,紫外检测波长分别为240、270 nm。结果:止血通明合剂薄层层析有明显的特征斑点。龙胆苦苷、黄芩苷、盐酸小檗碱、补骨脂素、异补骨脂素的质量浓度分别为467.29、232.87、123.12、148.89、142.56 μg/mL。结论:本研究建立的薄层色谱法和高效液相色谱法可用于制定止血通明合剂质量标准的方法学依据。
Abstract:
Objective: To provide theoretical reference for quality control of ZhiXue TongMing mixture by performing qualitative and quantitative analysis of the indicative components in the mixture detected. Methods: Diamond ODS C18(4.6 mm×250 mm, 5 μm) chromatographic column was used, acetonitrile-01% phosphoric acid (Adjusting pH value of triethylamine to 5.1) was chosen as the mobile phase in gradient elution, flow rate: 0.8 mL/min, column temperature:30℃, ultraviolet detection wavelength was 240 nm and 270 nm. Results: Characteristic spots of ZhiXue TongMing mixture were identified by TCL. Mass concentrations of gentiamarin, baicalin, berberine hydrochloride, psoralen and isopsoralen were 467.29, 232.87, 123.12, 148.89 and 142.56 μg/mL. Conclusion: TLC and HPLC set up in the study could be used as the reference of the methodology of establishing the quality standard of ZhiXue TongMing mixture.

备注/Memo

备注/Memo:
收稿日期:2018-06-27 *基金项目:兰州市2011年第二批科技计划项目(编号 2011-2-5)。 作者简介:杨彦彪(1970—),男,主任药师。研究方向:医院制剂研发与管理。
更新日期/Last Update: 2019-01-15