[1]郭洋洋.大蒜素对慢性肾衰大鼠肾脏组织保护作用的研究[J].西部中医药,2019,32(02):9-13.
　GUO Yangyang.Study of the Protective Effects of Allimin on Renal Tissue of the Rats with Chronic Renal Failure[J].Western Journal of Traditional Chinese Medicine,2019,32(02):9-13.
点击复制

大蒜素对慢性肾衰大鼠肾脏组织保护作用的研究
分享到：

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
32
期数:
2019年02期
页码:
9-13
栏目:
出版日期:
2019-02-15

文章信息/Info

Title:
Study of the Protective Effects of Allimin on Renal Tissue of the Rats with Chronic Renal Failure
文章编号:
1004-6852(2019)02-0009-05
作者:
郭洋洋
廊坊市第四人民医院，河北 廊坊 065700
Author(s):
GUO Yangyang
Langfang Municipality the Forth People′s Hospital, Langfang 065700, China
关键词:
大蒜素; 慢性肾衰; 肾脏组织; 肾脏指数; 氧化应激; 动物实验
Keywords:
allimin;  chronic renal failure;  renal tissue;  renal index;  oxidative stress
分类号:
R699.2
摘要:
目的：研究大蒜素对慢性肾衰(CRF)大鼠肾脏组织的保护作用并探讨其机制。方法：将100只实验大鼠随机分为正常对照组、模型对照组，大蒜素低、中、高剂量组，每组20只。采用腺嘌呤溶液[(250 mg/(kg·d)]灌胃21天的方法建立CRF大鼠模型，大蒜素各组腹腔注射大蒜素[5、10、20 mg/(kg·d)]进行治疗，正常对照组及模型对照组给予正常饮食，疗程28天。观察各组大鼠一般生存状态，测定体质量、24小时尿量、24小时尿蛋白量，计算肾脏指数，测定血清尿素氮(BUN)、血肌酐(SCr)、尿酸(UA)含量；观察肾脏组织病理变化及细胞凋亡指数(AI)，检测抗氧化酶［超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)］活性和丙二醛(MDA)含量。结果：与模型对照组比较，经大蒜素治疗28天能够有效改善CRF大鼠一般生存状态；大蒜素20 mg/(kg·d)组CRF大鼠体质量显著升高(P＜0.05)，大蒜素10、20 mg/(kg·d)组24小时尿量显著减少、24小时尿蛋白量显著降低(P＜0.05)，肾脏指数显著降低(P＜0.05)，血清BUN、SCr、UA含量显著降低(P＜0.05)；经大蒜素治疗能够明显改善CRF大鼠肾脏组织病变并抑制细胞凋亡，大蒜素10、20 mg/(kg·d)组AI显著降低(P＜0.05)，肾脏组织SOD活性显著升高，MDA含量显著降低(P＜0.05)，20 mg/(kg·d)组CAT、GSH-Px活性显著升高(P＜0.05)。结论：大蒜素对CRF大鼠肾脏组织具有一定的保护作用，可能与其能够降低氧化应激损伤、抑制细胞凋亡有关。
Abstract:
Objective：To study the protective effects of allimin on renal tissue of the rats with chronic renal failure (CRF), and initially survey the mechanism. Methods: All 100 rats were randomized into normal control group,model control group,low,moderate and high dosages groups of allimin,20 rats each group,Intragastric administration of adenine solution [250 mg/(kg·d)] for 21 days was used to build CRF rat models, they were treated by peritoneal injection of allimin [5, 10 and 20 mg/(kg·d)], normal control group and model control group accepted normal diet，the course was 28 days. The contents of MDA, GSH-Px, CAT and SOD were detected, apoptosis index(AI) and pathological changes of renal tissue were observed; the contents of UA, SCr and BUN in the serum were measured, renal index was calculated, 24 hours urinary protein excretion(24 hUPE), 24 hours urinary volume and body weight were detected, general living state of the rats in dfferent groups were observed. Results: Compared with model control group, treating with allimin for 28 days could improve general living state of CRF rats effectively; body weight of CRF rats in allimin group [20 mg/(kg·d)] rose significantly (P＜0.05), 24 h urinary volume of the rats in the allimin groups of 10 and 20 mg/(kg·d) reduced notably, and 24hUPE lowered remarkably (P＜0.05), renal index reduced notably (P＜0.05), the contents of BUN, SCr and UA decreased significantly (P＜0.05); allimin could obviously improve the pathological changes of renal tissue of CRF rats and inhibit cellular apoptosis, AI lowered notably in the allimin groups of 10 and 20 mg/(kg·d) (P＜0.05); the activity of SOD in renal tissue rose notably, and the contents of MDA decreased apparently(P＜0.05), the activity of CAT, GSH-Px of the group of 20 mg/(kg·d) rose signficantly(P＜0.05). Conclusion: Allimin possess certain protective effects to renal tissue of CRF rats, which might be related to decreasing oxidative stress injury and inhibiting cellular apoptosis.

相似文献/References:

[1]贾鸿扬,徐英△.张大宁教授应用茵陈失笑散治疗慢性肾功能衰竭验案[J].西部中医药,2016,29(03):68.
　JIA Hongyang,XU Ying.Professor Zhang Daning′s Effective Cases of Applying YinChen ShiXiao Powder to Chronic Renal Failure[J].Western Journal of Traditional Chinese Medicine,2016,29(02):68.
[2]赵肖帆,先小乐,牛家瑜,等.基于“整体功能代偿疗法”探讨增强肝的代偿功能对慢性肾衰模型大鼠肾功能的影响[J].西部中医药,2017,30(10):16.
　ZHAO Xiaofan,XIAN Xiaole,NIU Jiayu,et al.Discussion on the Effects of Enhancing Liver Compensatory Function on Renal Function of the Rats with Chronic Renal Failure based on Wholistic Functional Compensatory Therapy[J].Western Journal of Traditional Chinese Medicine,2017,30(02):16.
[3]朱敏杰.大蒜素对肾缺血再灌注损伤大鼠细胞凋亡的影响[J].西部中医药,2018,31(09):38.
　ZHU Minjie.Effects of Allimin on Cellular Apoptosis of the Rats with Renal Ischemia/Reperfusion Injury[J].Western Journal of Traditional Chinese Medicine,2018,31(02):38.
[4]张雪丹,陈振宙.大蒜素对链脲佐菌素所致糖尿病大鼠胰岛素抵抗及脂肪因子的影响[J].西部中医药,2019,32(01):23.
　ZHANG Xuedan,CHEN Zhenzhou.Effects of Allitridi on Insulin Resistance and Adipokines of the Rats with Streptozotocin-induced Diabetes Mellitus[J].Western Journal of Traditional Chinese Medicine,2019,32(02):23.
[5]郭燕军.大蒜素对人舌鳞状细胞癌Tca8113细胞生长的影响[J].西部中医药,2019,32(02):25.
　GUO Yanjun.Influence of Allimin on Tca8113 Cellular Growth of Human Tongue Squamous Cell Carcinoma[J].Western Journal of Traditional Chinese Medicine,2019,32(02):25.
[6]黄彩兰,指导：刘新.刘新教授治疗慢性肾衰经验总结[J].西部中医药,2019,32(07):83.
　HUANG Cailan,DirectorLIU Xin.Professor Liu Xin′s Experience of Treating Chronic Renal Failure[J].Western Journal of Traditional Chinese Medicine,2019,32(02):83.
[7]夏嘉,王颖,刘铭洁,等.抗纤灵对慢性肾功能衰竭模型Smad2-KO小鼠心脏CoI、CoIII、AngII、TNF-α、IL-6的影响[J].西部中医药,2021,34(02):1.[doi:10.12174/j.issn.2096-9600.2021.02.04]
　XIA Jia,WANG Ying,LIU Mingjie,et al.Influence of Kangxianling on the Expressions of CoI, CoIII, AngII, TNF-α and IL-6 in the Heart of Smad2-KO Mice of CRF Model[J].Western Journal of Traditional Chinese Medicine,2021,34(02):1.[doi:10.12174/j.issn.2096-9600.2021.02.04]
[8]曾露,张蕾,侯海晶,等.杨霓芝教授治疗不同原发病所致慢性肾功能衰竭用药规律[J].西部中医药,2024,37(01):59.[doi:10.12174/j.issn.2096-9600.2024.01.14]
　ZENG Lu,ZHANG Lei,HOU Haijing,et al.Professor Yang Nizhi 's Medication Rules of Treatment of Chronic Renal Failure Induced by Different Primary Diseases[J].Western Journal of Traditional Chinese Medicine,2024,37(02):59.[doi:10.12174/j.issn.2096-9600.2024.01.14]
[9]白敏,李晓翠,靳世英,等.基于TGF-β1/Smads信号通路探讨大蒜素对2型糖尿病大鼠肾纤维化的影响[J].西部中医药,2024,37(03):5.[doi:10.12174/j.issn.2096-9600.2024.03.02]
　BAI Min,LI Xiaocui,JIN Shiying,et al.Effect of Allicin on Renal Fibrosis in Type 2 Diabetic Rats Based on TGF-β1/Smads Signaling Pathway[J].Western Journal of Traditional Chinese Medicine,2024,37(02):5.[doi:10.12174/j.issn.2096-9600.2024.03.02]

备注/Memo

备注/Memo:
收稿日期：2018-07-08 *基金项目：廊坊市科技支撑计划项目(编号2016013136)。 作者简介：郭洋洋(1986—)，女，硕士学位，主治医师。研究方向：肾脏病。
更新日期/Last Update: 2019-02-15