[1]程丽飞,王华林,李敏△.四逆汤及其拆方水煎液对缺血心肌细胞的保护作用[J].西部中医药,2019,32(05):9-12.
　CHENG Lifei,WANG Hualin,LI Min.Protective Effects of SiNi Decoction and the Decoction of the Constituent Drugs on Ischemic Myocardial Cells[J].Western Journal of Traditional Chinese Medicine,2019,32(05):9-12.
点击复制

四逆汤及其拆方水煎液对缺血心肌细胞的保护作用
分享到：

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
32
期数:
2019年05期
页码:
9-12
栏目:
出版日期:
2019-05-15

文章信息/Info

Title:
Protective Effects of SiNi Decoction and the Decoction of the Constituent Drugs on Ischemic Myocardial Cells
文章编号:
1004-6852(2019)05-0009-04
作者:
程丽飞1，王华林2，李敏2△
1 陕西省交通医院，陕西 西安 710068； 2 陕西中医药大学
Author(s):
CHENG Lifei1, WANG Hualin2, LI Min2△
1 Shaanxi Traffic Hospital, Xi′an 710068, China; 2 Shaanxi University of Chinese Medicine
关键词:
MTT缺血模型; 心肌细胞; H9C2; 四逆汤及其拆方
Keywords:
MTT ischemic model;  myocardial cells;  H9C2;  SiNi decoction and the decoction of the constituent drugs
分类号:
R285.5
摘要:
目的：探讨四逆汤(Sini decoction，SND)及其拆方水煎液对缺血大鼠离体心肌细胞H9C2的保护作用。方法：采用不同浓度的含药培养液培养细胞，显微镜下观察心肌细胞建模前后的生长状态；利用MTT比色法比较各加药浓度下单味药、药对、全方对缺血前后心肌细胞H9C2存活率的影响，并分析各试药之间作用的差异。结果：1)400倍镜下可见正常培养细胞生长状态良好，细胞形态为梭形，聚集式生长；缺血模型细胞形态改变，间隙变大，结构发生萎缩，死亡细胞增多。2)与缺血后SND组比较，10、20、5 mg/mL浓度干姜水煎液(Ginger water decoction,GWD)对缺血后H9C2细胞无保护作用，存活率分别为（44.89±2.59）%、（49.43±1.87）%、（73.67±1.30）%，两两之间差异显著（P＜0.01）。在浓度为1.0 mg/mL时，附子水煎液(aconite water decoction,AWD)对缺血前后各组细胞均产生细胞毒性。甘草水煎液(Licorice water decoction,LWD)同附子-甘草水煎液(licorice and aconite decoction,LAWD)比较，对H9C2保护作用均较弱。附子-干姜水煎液(Ginger and aconite water decoction,GAWD)各浓度缺血后加药组同SND组比较对H9C2保护作用无差异。结论：SND及其拆方GAWD、LAWD、AWD、LWD均对缺血H9C2有明显促增殖作用；各浓度GWD对缺血H9C2无保护作用，1.0 mg/mL AWD有细胞毒性。
Abstract:
Objective: To study the protective effects of SiNi decoction(SND) and the decoction of the constituent drugs on isolated ischemic myocardial cells H9C2 of the rats. Methods: Different concentrations of medicated nutrient fluids were used to culture the cells, the growth conditions of myocardial cells were observed before and after establishing the models under the microscope; MTT chromatometry was used to compare the effects of single herb, herbal couplets and the whole prescriptions on survival rate of H9C2 before and after the ischemia, and analyze the difference among the test herbs. Results: 1) Under 400 times microscope, it could be seen that the growth of normal cells was good, cellular morphology was fusiformis, growing in clusters; cellular morphology of the ischemic cells present changes, the gap became large, the structure atrophied, and the dead cells increased. 2) Compared with SND group after ischemia, 10，20 and 5 mg/mL ginger water decoction (GWD) showed no protective effects on H9C2 cells after the ischemia, the survival rates were (44.89±2.59)%, (49.43±1.87)% and (73.67±1.30)% respectively, the difference was significant between both (P＜0.01). FuZi decoction (aconite water decoction, AWG) could produce cytotoxicity on the cells in different groups before and after ischemia when the decoction was in the concentrations of 1.0 mg/mL. When licorice water decoction (LWD) was compared with licorice and aconite decoction(LAWD), it showed weaker protective effects on H9C2. There was no difference when the medication groups of ginger and aconite water decoction (GAWD) in different concentrations after the ischemia was compared with SND group.Conclusion: SND and the decoction of the constituent drug pairs GAWD, LAWD, AWD and LWD could promote the proliferation of ischemic myocardial cells H9C2, different concentrations of GWD could not protect ischemic H9C2, while 1.0mg/mL AWD shows cytotoxicity.

相似文献/References:

[1]马妍,郭利平△.芪参益气滴丸预适应内皮细胞对心肌细胞缺氧损伤的延迟保护作用[J].西部中医药,2015,28(05):11.
[2]黄宜生,贾钰华△,糜小英.酸枣仁皂苷A预处理对过氧化氢诱导大鼠心肌细胞凋亡的作用[J].西部中医药,2013,26(07):8.
　HUANG Yisheng,JIA Yuhua,MI Xiaoying.Effects of Jujuboside A Preconditioning on Myocardial Cell Apoptosis Induced by Hydrogen Peroxide in Rat[J].Western Journal of Traditional Chinese Medicine,2013,26(05):8.
[3]陈灵,周汉明△.五味子乙素介导Traf6/NF-κB信号通路抑制心肌细胞肥大实验研究[J].西部中医药,2020,33(01):33.[doi:10.12174/j.issn.1004-6852.2020.01.09]
　CHEN Ling,ZHOU Hanming.Experimental Research on Inhibitory Effects of Schisandrin B on Cardiomyocyte Hypertrophy through Mediating Traf6/NF-κB Signal Channel[J].Western Journal of Traditional Chinese Medicine,2020,33(05):33.[doi:10.12174/j.issn.1004-6852.2020.01.09]
[4]王玲,黄佳.五味子乙素调控Jak2/Stat3信号通路抑制心肌细胞氧化应激损伤[J].西部中医药,2020,33(02):11.[doi:10.12174/j.issn.1004-6852.2020.02.03]
　WANG Ling,HUANG Jia.Schisandrin B Regulating Jak2/Stat3 Signaling Pathway in the Inhibitionof Oxidative Stress Damage of Myocardial Cells[J].Western Journal of Traditional Chinese Medicine,2020,33(05):11.[doi:10.12174/j.issn.1004-6852.2020.02.03]
[5]邢俊娥,吴鹏,李海沅.益气活血方对心衰大鼠心肌细胞线粒体功能的影响及其机制探讨[J].西部中医药,2022,35(07):23.[doi:10.12174/j.issn.2096-9600.2022.07.06]
　XING Jun'e,WU Peng,LI Haiyuan.Effects of Qi-invigorating Blood-activating Prescription on Mitochondrial Function of Myocardial Cells in Rats with Heart Failure and Exploration into the Mechanism[J].Western Journal of Traditional Chinese Medicine,2022,35(05):23.[doi:10.12174/j.issn.2096-9600.2022.07.06]
[6]李兵.丹参多酚酸盐对H2O2诱导心肌细胞凋亡与自噬的影响及机制研究[J].西部中医药,2022,35(10):29.[doi:10.12174/j.issn.2096-9600.2022.10.07]
　LI Bing.Influence of SAL on H2O2-Induced Apoptosis and Autophagy of H9c2 Myocardial Cells and the Mechanism Study[J].Western Journal of Traditional Chinese Medicine,2022,35(05):29.[doi:10.12174/j.issn.2096-9600.2022.10.07]

备注/Memo

备注/Memo:
收稿日期：2018-12-09 *基金项目：国家自然科学基金项目(编号81303243)；陕西省自然科学基础研究计划项目(编号2017JM8080)；陕西省中医药管理局项目(编号JCPT007)。 作者简介：程丽飞(1987—)，女，硕士学位，主管药师。研究方向：临床中药学。 △通讯作者：李敏(1978—)，女，博士学位，教授。研究方向：临床中药学。
更新日期/Last Update: 2019-05-15